The Effect of Low Calcium Diet on Bone in Ovariectomized Mice.

Minematsu, Akira; Yoshimura, Osamu; Yotsuji, Hirofumi; Ichigo, Hirozo; Takayanagi, Kiyomi; Kobayashi, Ryuji; Hosoda, Masataka; Sasaki, Hisato; Maejima, Hiroshi; Matsuda, Yuichi; Tanaka, Sachiko; Kanemura, Naohiko; Matsuo, Akihisa

doi:10.1298/jjpta.3.13

Cited by 3 publications

(3 citation statements)

References 16 publications

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“…In our work, such a low-calcium diet was provided to speed up the osteopenic process, resulting in an approximately 6-week reduction to achieve an osteoporotic condition compared to the model with normal diet. 61,62 Bone volume, trabecular thickness and number were also in accordance with previous studies, 49,63 highlighting the successful establishment of an osteoporotic bone condition.…”

Section: Discussionsupporting

confidence: 89%

Influence of bisphosphonate treatment on bone substitute performance in osteoporotic conditions

Camargo

Hoekstra

Jansen

et al. 2023

Clin Implant Dent Rel Res

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Objective: Considering the elevated number of osteoporotic patients in need of bone graft procedures, we here evaluated the effect of alendronate (ALN) treatment on the regeneration of bone defects in osteoporotic rats. Bone formation was histologically and histomorphometrically assessed in rat femoral condyle bone defects filled with bone graft (Bio-Oss ® ) or left empty.Methods: Male Wistar rats were induced osteoporotic through orchidectomy (ORX) and SHAM-operated. The animals were divided into three groups: osteoporotic (ORX), osteoporotic treated with ALN (ORX + ALN) and healthy (SHAM). Six weeks after ORX or SHAM surgeries, bone defects were created bilaterally in femoral condyles; one defect was filled with Bio-Oss ® and the other one left empty. Bone regeneration within the defects was analyzed by histology and histomorphometry after 4 and 12 weeks.Results: Histological samples showed new bone surrounding Bio-Oss ® particles from week 4 onward in all three groups. At week 12, the data further showed that ALN treatment of osteoporotic animals enhanced bone formation to a 10-fold increase compared to non-treated osteoporotic control. Bio-Oss ® filling of the defects promoted bone formation at both implantation periods compared to empty controls. Conclusion:Our histological and histomorphometric results demonstrate that the enteral administration of alendronate under osteoporotic bone conditions leverages bone defect regeneration to a level comparable to that in healthy bone. Additionally, Bio-Oss ® is an effective bone substitute, increasing bone formation, and acting as an osteoconductive scaffold guiding bone growth in both healthy and osteoporotic bone conditions.Significance: Based on the results of this study, enteral use of ALN mitigates adverse effects of an osteoporotic condition on bone defect regeneration.

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Section: Discussionsupporting

confidence: 89%

Influence of bisphosphonate treatment on bone substitute performance in osteoporotic conditions

Camargo

Hoekstra

Jansen

et al. 2023

Clin Implant Dent Rel Res

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“…In animals, low Ca intake induces bone loss and the reduction of bone formation rate [5][6][7] . The ovariectomized (OVX) animal is well known for osteoporotic model, and widely used 8)9) .…”

mentioning

confidence: 99%

Time Course of Influence by Ovariectomy and Calcium Diet on Bone Properties in Mice.

Minematsu

Yoshimura

Yotsuji

et al. 2001

J Jpn Phys Ther Assoc

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This study was investigated the influence by ovariectomy (OVX) and calcium diet on bone properties in eighty-one female ICR strain mice with age of 5 weeks. The animals were randomly assigned to sham operation (SHAM), OVX, SHAM+low Ca intake (L.Ca) and OVX+L.Ca group. They were euthanized with lethal dose of pentobarbital sodium at day 50, 100 and 140 post-operatively. For determining the bone properties, both femur and tibial bones were excised from the hind limb, and removed off surrounding tissues. Thereafter, bone length, bone dry weight, and also mechanical strength and ash content of the bones were determined. The bone length on both femur and tibia was significantly longer in OVX group than in the other groups after 50 day of experiment, this situation was continued to the end of the experiment. Bone dry weight, mechanical strength, and ash content were significantly decreased by OVX and L.Ca over the time of the experiment, and those of OVX+L.Ca group were the lowest in all groups. OVX and L.Ca have a great potential for weakening the mechanical strength and have an additive effect when combined. OVX and L.Ca block the gain of bone mass.

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“…Finally, a comment has to be made regarding the experimental conditions of the current study. Some authors noticed that a high calcium diet may reverse the condition of osteopenia in ovariectomized animals [ 49 ]. In our model, OVX rats were fed a standard diet with a considerable amount of calcium.…”

Section: Discussionmentioning

confidence: 99%

Histomorphometric Evaluation of Peri-Implant Bone Response to Intravenous Administration of Zoledronate (Zometa®) in an Osteoporotic Rat Model

et al. 2020

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We evaluated the response to peri-implant bone placed in the femoral condyle of osteoporotic rats, following intravenous zoledronate (ZOL) treatment in three settings: pre-implantation (ZOL-Pre), post-implantation (ZOL-Post), and pre- + post-implantation (ZOL-Pre+Post). Twenty-four female Wistar rats were ovariectomized (OVX). After 12 weeks, the rats received titanium implants in the right femoral condyle. ZOL (0.04 mg/kg, weekly) was administered to six rats 4 weeks pre-implantation and was stopped at implant placement. To another six rats, ZOL was given post-implantation and continued for 6 weeks. Additional six rats received ZOL treatment pre- and post-implantation. Control animals received weekly saline intravenous injections. At 6 weeks post-implantation, samples were retrieved for histological evaluation of the percentage of bone area (%BA) and of the percentage of bone-to-implant contact (%BIC). BA% for ZOL-Pre (29.6% ± 9.0%) and ZOL-Post (27.9% ± 5.6%) rats were significantly increased compared to that of the controls (17.3% ± 3.9%, p < 0.05). In contrast, ZOL-Pre+Post rats (20.4% ± 5.0%) showed similar BA% compared to Saline controls (p = 0.731). BIC% revealed a significant increase for ZOL-Post (65.8% ± 16.9%) and ZOL-Pre+Post (68.3% ± 10.0%) rats compared with that of Saline controls (43.3% ± 9.6%, p < 0.05), while ZOL-Pre rats (55.6% ± 19%) showed a BIC% comparable to that of Saline controls (p = 0.408). Our results suggest that receiving intravenous ZOL treatment before or after implant placement enhances peri-implant bone responses in terms of bone area. However, the effect of different ZOL treatment regimens on BIC% was found to be inconclusive.

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The Effect of Low Calcium Diet on Bone in Ovariectomized Mice.

Cited by 3 publications

References 16 publications

Influence of bisphosphonate treatment on bone substitute performance in osteoporotic conditions

Influence of bisphosphonate treatment on bone substitute performance in osteoporotic conditions

Time Course of Influence by Ovariectomy and Calcium Diet on Bone Properties in Mice.

Histomorphometric Evaluation of Peri-Implant Bone Response to Intravenous Administration of Zoledronate (Zometa®) in an Osteoporotic Rat Model

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