The effect of locally administered phenytoin on wound healing in an experimental nasal septal perforation animal model

Çakan, Doğan; Uşaklıoğlu, Semih

doi:10.1007/s00405-022-07276-z

Cited by 6 publications

(10 citation statements)

References 38 publications

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“…7,24 Similar to previous studies, we completed the present experiment at the end of the 14th day. [25][26][27] MLT has positive effects on all stages of wound healing as demonstrated by previous studies in the literature. The inflammatory phase is the part of the wound healing process that begins shortly after injury, and its main function is to prevent infection.…”

Section: Discussionsupporting

confidence: 71%

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The Effect of Melatonin on Nasal Septal Wound Healing in an Experimental Animal Model

Çakan

Uşaklıoğlu

2022

Facial Plast Surg

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Objective: The purpose of our study was to examine the effect of melatonin (MLT) on wound healing in the nasal septum. Methods: Twenty-two Sprague–Dawley rats of the male sex were included in this experimental study. Nasal septal perforation (NSP), about two millimeters in diameter, was formed in each rat. Melatonin was applied to the subjects in the study group and saline was applied to the subjects in the control group once a day for 14 days. On day 14, the rats were sacrificed and the nasal septums of the subjects were resected for pathological evaluation. In the NSP area, degeneration and regeneration of nasal septal epithelium, degeneration and regeneration of the septal cartilage, number of cells involved in wound healing such as acute inflammatory cells (leukocytes), fibroblast, eosinophil, and giant cell, capillary vessel intensity, granulation tissue formation, collagen intensity parameters were evaluated histopathologically. The macroscopic size and histopathologic examination results of NSPs were statistically analyzed. Results: In the MLT group, the epithelium regeneration, the cartilage regeneration, fibroblast number, collagen density, vascularity, and granulation formation were significantly higher, and the epithelial degeneration and acute inflammatory cells density were significantly lower, in the NSP area (p<0.05). In addition, the macroscopic healing was significantly higher in the MLT group (p= 0.044, p<0.05) Conclusion: The local application of melatonin accelerates nasal septal wound healing. With this feature, melatonin may use to prevent the formation of NSP.

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Section: Discussionsupporting

confidence: 71%

“…Due to its low water solubility, MLT was mixed with vaseline which was used in previous studies on nasal wound healing (7,24). Similar to previous studies, we completed the present experiment at the end of the 14 th day (25,26,27).…”

Section: Accepted Manuscriptmentioning

confidence: 94%

The Effect of Melatonin on Nasal Septal Wound Healing in an Experimental Animal Model

Çakan

Uşaklıoğlu

2022

Facial Plast Surg

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“…Previous studies have shown that WH improver medicinal agents are effective in the healing of NSP. 7,19 The present study showed that LF affects WH positively by decreasing acute inflammation and increasing epithelium regeneration, fibroblast number, collagen density, and granulation formation. In addition to microscopic changes, it increased the healing of perforations macroscopically.…”

Section: Discussionmentioning

confidence: 50%

“…Subjects were placed with the left nasal cavity down to prevent aspiration and protect the right side for breathing. 19…”

Section: Methodsmentioning

confidence: 99%

The Efficacy of Topical Lactoferrin on Wound Healing in an Experimental Animal Model of Nasal Septum Perforation

Uşaklıoğlu

Çakan

2023

Facial Plast Surg

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The aim of the present experimental animal study was to investigate the efficacy of bovine lactoferrin (LF) on wound healing in an animal model of nasal septum perforation (NSP).Twenty-two, 8 to 10 weeks of age, male Sprague-Dawley rats were separated into two groups. Nasal septum perforation was created in each rat. The saline (control) and 0.05 mg LF (study) groups were delivered locally for 14 days. On the 14th day of the study, after the sacrifice, the cartilage nasal septa of the animals were excised. The degeneration and regeneration observed in the nasal septum epithelium and cartilage, the number of acute inflammatory cells, the number of eosinophils, the amount of new vessel formation, the amount of granulation, and the collagen density were examined microscopically. The microscopic parameters and macroscopic healing of NSPs were analyzed. The epithelium regeneration, the fibroblast number, the granulation tissue formation, the collagen density, and the macroscopic healing were significantly higher in the LF group (p < 0.05). Besides, the acute inflammatory cell count was lower in the LF group (p = 0.034). In conclusion, the topically delivered LF can improve wound healing in an experimental rat model of NSP.

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“…Однако в связи с интенсивным развитием малоинвазивной хирургии актуальным остается вопрос изучения основных звеньев патогенеза нарушений регенерации МЦС носа при воспалительной реакции СО после оперативных вмешательств. На сегодня недостаточно изучен характер патоморфологических изменений СО в процессе заживления, а также основные причины нарушения репарации, способствующие стойкой морфологической трансформации СО [18][19][20][21][22][23][24][25]. На наш взгляд, оптимальным решением данной проблемы может стать разработка и использование методик экспериментального ринита у лабораторных животных.…”

Section: Introductionunclassified

Evaluating the Developed Model of Experimental Rhinitis in Laboratory Rats: Pre-Clinical Experimental Randomized Study

Berest

Tananakina

Teleshova

et al. 2023

Kuban. nauсh. med. vestnik

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Background. Among the damaging factors affecting the mucociliary system of the nasal cavity, surgical wound is of particular relevance in the practice of an otorhinolaryngologist. The clinical assessment of regeneration of the mucociliary system is associated with certain difﬁ culties, since the intravital morphological examination of the nasal mucosa in patients is traumatic. Therefore, the development of animal models of experimental rhinitis is considered to be highly relevant in order to study the dynamics of mucociliary pathomorphological changes and assess the epithelium regeneration.Objectives. To evaluate the developed model of experimental rhinitis in laboratory rats by studying clinical, morphological and biochemical changes in the inﬂ ammatory process.Methods. The experimental rhinitis model was developed and tested on 60 mature male Wistar rats. All animals were randomized into two groups: experimental group #1 (n = 30) — rats in which experimental rhinitis modeling was performed and group #2 (n = 30) — control, intact animals. In the course of the experiment, the authors examined the content of CRP in blood, evaluated the differential blood cell count, and studied a morphology of the nasal septum mucosa in 2, 5, 10 days after the injury to assess the dynamics of the inﬂ ammatory process in rats of both groups. Statistical analysis of the study results was carried out by means of Statistica 8.0 (StatSoft Inc., USA).Results. After injury, the rats from group #1 developed acute rhinitis, which was clinically manifested by the release of mucous or mucopurulent secretion from the nostrils, sneezing and scratching the nose. An increase in CRP, band and segmented neutrophils, and a decrease in lymphocytes were observed in blood of the rats from group #1 in comparison with the control group. Microscopic analysis of changes in the nasal septum mucosa showed that the acute phase of exudative inﬂ ammation developed on the second day: vascular congestion, edema, neutrophilic inﬂ ammatory inﬁ ltration of the submucosal membrane were observed against the background of foci of epithelial necrosis. The proportion of lymphocytes and macrophages in the inﬂ ammatory inﬁ ltrate increased by the ﬁ fth day, initial signs of restoration of epithelial tissue — the formation of an undifferentiated regenerating epithelium — appeared by the tenth day.Conclusion. The results of the study show that an adequate experimental model of acute rhinitis in laboratory animals have been obtained. An acute inﬂ ammatory process is characterized by clinical manifestations and changes in blood parameters. Particular destructive and reparative-proliferative changes develop in the mucous membrane of the nasal septum of experimental animals as a result of a surgical wound.

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The effect of locally administered phenytoin on wound healing in an experimental nasal septal perforation animal model

Cited by 6 publications

References 38 publications

The Effect of Melatonin on Nasal Septal Wound Healing in an Experimental Animal Model

The Effect of Melatonin on Nasal Septal Wound Healing in an Experimental Animal Model

The Efficacy of Topical Lactoferrin on Wound Healing in an Experimental Animal Model of Nasal Septum Perforation

Evaluating the Developed Model of Experimental Rhinitis in Laboratory Rats: Pre-Clinical Experimental Randomized Study

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