The effect of lightly crosslinked poly(carboxybetaine) hydrogel coating on the performance of sensors in whole blood

Yang, Wei; Bai, Tao; Carr, Louisa R.; Keefe, Andrew J.; Xu, Jiu Jun; Xue, Hong; Irvin, Colleen A.; Chen, Shengfu; Wang, Joseph; Jiang, Shaoyi

doi:10.1016/j.biomaterials.2012.07.035

Cited by 71 publications

(55 citation statements)

References 27 publications

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“…For the lightly cross-linked pCB hydrogel, proteins can freely diffuse in and out of the matrix with no conformation changes. [72] pCB-based hydrogel coating on the glucose sensor was demonstrated to achieve the long-term Figure 4. Two approaches to prepare pCB hydrogels.…”

Section: Pcb Materials On Soft Surfacesmentioning

confidence: 99%

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Recent advances of zwitterionic carboxybetaine materials and their derivatives

Cao

Tang

Cheng

2014

Journal of Biomaterials Science, Polymer Edition

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Zwitterionic polycarboxybetaine (pCB)-based materials have drawn special attention due to their outstanding properties of resisting proteins adsorption, biofilm formation and cell attachment on a variety of substrates and surfaces, reducing the uptake of pCB-coated nanosized materials, as well as providing the capability of further functionalization. Zwitterionic pCBs have been proven to be excellent choices for many applications, not only limited to the field of antifouling. This review will summarize the recent progress on the development of pCB materials and the understanding of the structure-function-property relationships of carboxybetaine materials.

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Section: Pcb Materials On Soft Surfacesmentioning

confidence: 99%

“…They have been used to protect implantable electrochemical glucose biosensors from biofouling in complex media, [71,72] and enhance linearity and sensitivity of the sensing profile. For the lightly cross-linked pCB hydrogel, proteins can freely diffuse in and out of the matrix with no conformation changes.…”

Section: Pcb Materials On Soft Surfacesmentioning

confidence: 99%

Recent advances of zwitterionic carboxybetaine materials and their derivatives

Cao

Tang

Cheng

2014

Journal of Biomaterials Science, Polymer Edition

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“…3,10 For example, Yang et al demonstrated a glucose sensor coated with crosslinked CB hydrogel having extraordinary performance in whole blood and long-term stability for up to 42 days. 13 The effectiveness of zwitterionic materials comes mainly from their strong hydration and charge balancing ability, leading to a reduction in the entropy gain and a) Electronic mail: cjhuang@ncu.edu.tw b)…”

Section: Introductionmentioning

confidence: 99%

Natural zwitterionic organosulfurs as surface ligands for antifouling and responsive properties

et al. 2014

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Natural sulfur-containing zwitterionic compounds, l-cysteine (Cys), l-methionine, and glutathionine (GSH), have been employed as surface ligands to prevent protein nonspecific adsorption on planar substrates. These organosulfur compounds form self-assembled monolayers (SAMs) on gold substrates by gold–sulfur interaction. The chemical elements of SAMs were confirmed using x-ray photoelectron spectroscopy. The surface wetting tests for SAMs show that films prepared from Cys and GSH exhibited super-hydrophilicity (contact angles of θ = ∼5°) due to their high coverage and strong hydration via ionic solvation and formation of hydrogen bonding. Quartz crystal microbalance with dissipation sensor was used to quantitatively and qualitatively monitor the adsorption of bovine serum albumin (BSA) from buffer onto these SAMs. It was found that the GSH film enables the resistance of BSA adsorption to the best extent at a physiological pH. Moreover, the surface charges of modified substrates were modulated by varying the pH value to control BSA adsorption. The effect of electrostatic repulsion on the antifouling behavior becomes prominent at a pH where the protein and the surface carry same charges. Consolidating the BSA adsorption measurements at different pH values, the antifouling properties of GSH-modified Au should be attributed to prevention of entropy gain and enthalpy loss, making BSA adsorption energetically unfavorable. It is believed that the surface modification with natural organosulfur ligands holds great potential in improving the biocompatibility of medical devices and in offering intelligent biointerfaces in response to environmental stimuli.

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“…Zwitterionic materials bind water via electrostatic interactions as opposed to PEG, which exploits H-bonding forces. Given the strength of electrostatic interactions, zwitterionic-induced hydration is much stronger [54], leading to minimal (if any) protein adsorption on surfaces coated with these materials [55]. Poly(sulfobetaine) (pSB) and poly(carboxybetaine) (pCB) are two common types of zwitterionic materials, but pCB is more attractive due to its potential for functionalization with aminated biomolecules via simple carbodiimide chemistry given its free carboxylate groups.…”

Section: Discussionmentioning

confidence: 99%

Effect of anticoagulants on the protein corona-induced reduced drug carrier adhesion efficiency in human blood flow

Sobczynski

Eniola‐Adefeso

2017

Acta Biomaterialia

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Plasma proteins rapidly coat the surfaces of particulate drug carriers to form a protein corona upon their injection into the bloodstream. The high presence of immunoglobulins in the corona formed on poly(lactic-co-glycolic acid) (PLGA) vascular-targeted carrier (VTC) surfaces was recently shown to negatively impact their adhesion to activated endothelial cells (aECs) in vitro. Here, we characterized the influence of anticoagulants, or their absence, on the binding efficiency of VTCs of various materials via modulation of their protein corona. Specifically, we evaluated the adhesion of PLGA, poly(lactic acid) (PLA), polycaprolactone (PCL), silica, and polystyrene VTCs to aECs in heparinized, citrated, and non-anticoagulated (serum and whole) blood flows relative to buffer control. Particle adhesion is substantially reduced in non-anticoagulated blood flows regardless of the material type while only moderate to minimal reduction is observed for VTCs in anticoagulant-containing blood flow depending on the anticoagulant and material type. The substantial reduction in VTC adhesion in blood flows was linked to a high presence of immunoglobulin-sized proteins in the VTC corona via SDS-PAGE analysis. Of all the materials evaluated, PLGA was the most sensitive to plasma protein effects while PCL was the most resistant, suggesting particle hydrophobicity is a critical component of the observed negative plasma protein effects. Overall, this work demonstrates that anticoagulant positively alters the effect of plasma proteins in prescribing VTC adhesion to aECs in human blood flow, which has implication in the use of in vitro blood flow assays for functional evaluation of VTCs for in vivo use.

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The effect of lightly crosslinked poly(carboxybetaine) hydrogel coating on the performance of sensors in whole blood

Cited by 71 publications

References 27 publications

Recent advances of zwitterionic carboxybetaine materials and their derivatives

Recent advances of zwitterionic carboxybetaine materials and their derivatives

Natural zwitterionic organosulfurs as surface ligands for antifouling and responsive properties

Effect of anticoagulants on the protein corona-induced reduced drug carrier adhesion efficiency in human blood flow

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