The effect of ligand density on in vivo tumor targeting of nanographene oxide

Lee, Jong Hyun; Sahu, Abhishek; Jang, Cheol Seong; Tae, Giyoong

doi:10.1016/j.jconrel.2015.04.035

Cited by 46 publications

(35 citation statements)

References 38 publications

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“…Nanomaterials' surface can be further tailored with targeting moieties in order to raise their specificity towards cancer cells. As previously substantiated for CNT, nGO, gold, Cu, W, Fe and PANI‐based nanomaterials, the outcome of the PTT mediated by these nanostructures can be improved by targeting receptors overexpressed on the surface of cancer cells . Liang et al verified that by grafting anti‐CD44 antibodies on PEGylated gold nanostars, their accumulation in tumor is enhanced by more than 2‐fold and, simultaneously, their uptake by RES organs is reduced .…”

Section: Approaches Used To Improve Nanomaterials Accumulation In Tumorsmentioning

confidence: 85%

“…Yet, the functionalization of nanomaterials is still regarded as a complex and a delicate process. In some cases, the complete functionalization of nanomaterials' surface does not guarantees an improvement of nanomaterials accumulation in tumors . In fact, targeted nanomaterials demand an appropriate ligand density for accomplishing such purpose.…”

Section: Approaches Used To Improve Nanomaterials Accumulation In Tumorsmentioning

confidence: 99%

“…In fact, targeted nanomaterials demand an appropriate ligand density for accomplishing such purpose. Lee et al verified that nGO coated with Pluronic F‐127‐folate conjugates with folate densities of 50 and 100% display a similar tumor‐homing capacity . In contrast, nanomaterials functionalized with lower folate densities (10 and 25%) exhibited a tumor accumulation comparable to that of non‐folate functionalized nGO, and, therefore, mediated a weaker photothermal effect.…”

Section: Approaches Used To Improve Nanomaterials Accumulation In Tumorsmentioning

confidence: 99%

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Strategies to Improve Cancer Photothermal Therapy Mediated by Nanomaterials

Melo‐Diogo

Pais-Silva

Dias

et al. 2017

Adv Healthcare Materials

220

164

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The deployment of hyperthermia-based treatments for cancer therapy has captured the attention of different researchers worldwide. In particular, the application of light-responsive nanomaterials to mediate hyperthermia has revealed promising results in several pre-clinical assays. Unlike conventional therapies, these nanostructures can display a preferential tumor accumulation and thus mediate, upon irradiation with near-infrared light, a selective hyperthermic effect with temporal resolution. Different types of nanomaterials such as those based on gold, carbon, copper, molybdenum, tungsten, iron, palladium and conjugated polymers have been used for this photothermal modality. This progress report summarizes the different strategies that have been applied so far for increasing the efficacy of the photothermal therapeutic effect mediated by nanomaterials, namely those that improve the accumulation of nanomaterials in tumors (e.g. by changing the corona composition or through the functionalization with targeting ligands), increase nanomaterials' intrinsic capacity to generate photoinduced heat (e.g. by synthesizing new nanomaterials or assembling nanostructures) or by optimizing the parameters related to the laser light used in the irradiation process (e.g. by modulating the radiation wavelength). Overall, the development of new strategies or the optimization and combination of the existing ones will surely give a major contribution for the application of nanomaterials in cancer PTT.

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Section: Approaches Used To Improve Nanomaterials Accumulation In Tumorsmentioning

confidence: 85%

Section: Approaches Used To Improve Nanomaterials Accumulation In Tumorsmentioning

confidence: 99%

Section: Approaches Used To Improve Nanomaterials Accumulation In Tumorsmentioning

confidence: 99%

See 1 more Smart Citation

Strategies to Improve Cancer Photothermal Therapy Mediated by Nanomaterials

Melo‐Diogo

Pais-Silva

Dias

et al. 2017

Adv Healthcare Materials

220

164

View full text Add to dashboard Cite

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“…Coating the nanoparticle surface with both a subcellular-targeting ligand and a tumor cell-targeting ligand might reduce the targeting efficiency of the latter, thereby even decreasing the accumulation of the drug in tumors 13 , which explains why few studies have reported the in vivo application of the dual-ligand modification strategy 9 . Moreover, the modification process is complicated by the potential effects of ligand density on targeting efficiency, and so careful screening is needed to determine the density of each ligand that will maximize targeting efficiency 14,15 .…”

Section: Introductionmentioning

confidence: 99%

Two birds, one stone: dual targeting of the cancer cell surface and subcellular mitochondria by the galectin-3-binding peptide G3-C12

Sun

et al. 2017

Acta Pharmacol Sin

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Active tumor-targeting approaches using specific ligands have drawn considerable attention over the years. However, a single ligand often fails to simultaneously target the cancer cell surface and subcellular organelles, which limits the maximum therapeutic efficacy of delivered drugs. We describe a polymeric delivery system modified with the G3-C12 peptide for sequential dual targeting. In this study, galectin-3-targeted G3-C12 peptide was conjugated onto the N-(2-hydroxypropyl) methacrylamide (HPMA) copolymer for the delivery of D(KLAKLAK)2 (KLA) peptide. G3-C12-HPMA-KLA exhibited increased receptor-mediated internalization into galectin-3-overexpressing PC-3 cells. Furthermore, G3-C12 peptide also directed HPMA-KLA conjugates to mitochondria. This occurred because the apoptosis signal triggered the accumulation of galectin-3 in mitochondria, and the G3-C12 peptide that specifically bound to galectin-3 was trafficked along with its receptor intracellularly. As a result, G3-C12-HPMA-KLA disrupted the mitochondrial membrane, increased the generation of reactive oxygen species (ROS) and induced cytochrome c release, which ultimately resulted in enhanced cytotoxicity. An in vivo study revealed that the G3-C12 peptide significantly enhanced the tumor accumulation of the KLA conjugate. In addition, G3-C12-HPMA-KLA exhibited the best therapeutic efficacy and greatly improved the animal survival rate. Our work demonstrates that G3-C12 is a promising ligand with dual-targeting functionality.

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“…These were also observed in vivo study, i.e., GO NP greatly inhibited the growth of tumor as shown in Figure . It seems to be that GO accelerates tumor accumulation of Ce6 and has potential in tumor targeting in vivo with low dependency of ligand density . We concluded that tumor targeting of GO NP can be achieved by enhanced permeation and retention (EPR) effect as shown in Figure because GO NP has no targeting ligand on the surface in our system.…”

Section: Discussionmentioning

confidence: 99%

Redox‐Responsive Nanocomposites Composed of Graphene Oxide and Chlorin e6 for Photodynamic Treatment of Cholangiocarcinoma

Kim

Lee

et al. 2018

Bulletin Korean Chem Soc

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The aim of this study is to synthesize graphene oxide (GO) nanocomposites conjugated with chlorin e6 (Ce6) and methoxy poly(ethylene glycol) (MePEG) using disulfide linkage for photodynamic treatment of cholangiocarcinoma (CCA) cells. Graphene oxide was conjugated with MePEG and cystamine (GO-(NSSNH 2 )-PEG). After that, amine end group was further conjugated with Ce6. Ce6 and MePEG conjugated GO nanocomposites (GO NP) were characterized and its photodynamic effect was studied with various CCA cells. In morphological observation, GO NP formed nano-aggregates with small sizes less than 100 nm while GO itself showed typical sheet shape. Ce6 release from GO NP was faster in the presence of glutathione, indicating that GO NP has redox-responsiveness. GO NP showed significantly higher intracellular Ce6 uptake ratio and produced higher amount of ROS than Ce6 itself. Then, GO NP resulted in increased anticancer activity against CCA cells compared to Ce6 itself. In animal study with SNU478 cell-bearing mice, GO NP was properly concentrated in tumor tissue while Ce6 treatment showed relatively higher fluorescence intensity in liver than that of tumor tissue. Tumor growth was significantly inhibited by treatment of GO NP with irradiation compared to Ce6 treatment. GO NP showed redoxresponsiveness and increased photodynamic effect against CCA cells. We suggest GO NP as a promising candidate for photodynamic therapy (PDT) of CCA cells.

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The effect of ligand density on in vivo tumor targeting of nanographene oxide

Cited by 46 publications

References 38 publications

Strategies to Improve Cancer Photothermal Therapy Mediated by Nanomaterials

Strategies to Improve Cancer Photothermal Therapy Mediated by Nanomaterials

Two birds, one stone: dual targeting of the cancer cell surface and subcellular mitochondria by the galectin-3-binding peptide G3-C12

Redox‐Responsive Nanocomposites Composed of Graphene Oxide and Chlorin e6 for Photodynamic Treatment of Cholangiocarcinoma

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