The Effect of Intrarenal Nickel Subsulfide Injections upon the Activity of Selected Erythrocyte and Bone Marrow Enzymes in Rats

Miszta, H; Dabrowski, Z; Szyguła, Zbigniew; Simon, Krzysztof

doi:10.1111/j.1600-0773.1986.tb00194.x

Cited by 3 publications

(2 citation statements)

References 5 publications

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“…The background of findings concerning the role of Ni in AChE activity is contradictory. Rat erythrocyte and bone marrow AChE activities were both found increased after a single intrarenal injection of Ni subsulfide (Ni 3 S 2 ; by approximately +47% and +67% towards their respective controls) [42]. In a more recent study, long-term (10 weeks) sludgesupplemented diet-fed rats (considered to be exposed to Ni, Cd, and other metals) were found to have activated brain and muscle AChE [43]; however, this study did not discriminate the effects of Ni from those deriving from the exposure to all sludgecontaining contaminants.…”

Section: Discussionmentioning

confidence: 93%

Short-Term Exposure to Nickel Alters the Adult Rat Brain Antioxidant Status and the Activities of Crucial Membrane-Bound Enzymes: Neuroprotection by L-Cysteine

Liapi

Ζάρρος

Theocharis

et al. 2011

Biol Trace Elem Res

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Nickel (Ni) is an environmental pollutant towards which human exposure can be both occupational (mainly through inhalation) and dietary (through water and food chain-induced bioaccumulation). The aim of this study was to investigate the effects of short-term Ni-administration (as NiCl(2), 13 mg/kg) on the adult rat whole brain total antioxidant status (TAS) and the activities of acetylcholinesterase (AChE), Na(+),K(+)-ATPase, and Mg(2+)-ATPase; in addition, the potential effect of the co-administration of the antioxidant L-cysteine (Cys, 7 mg/kg) on the above parameters was studied. Twenty-eight male Wistar rats were divided into four groups: A (saline-treated control), B (Ni), C (Cys), and D (Ni and Cys). All rats were treated once daily with intraperitoneal injections of the tested compounds, for 1-week. Rats were sacrificed by decapitation and the above-mentioned parameters were measured spectrophotometrically. Rats treated with Ni exhibited a significant reduction in brain TAS (-47%, p < 0.001, BvsA) that was efficiently limited by the co-administration of Cys (-4%, p > 0.05, DvsA; +83%, p < 0.001, DvsB), while Cys (group C) had no effect on TAS. The rat brain AChE activity was found significantly increased by both Ni (+30%, p < 0.001, BvsA) and Cys (+62%, p < 0.001, CvsA), while it tended to adjust to control levels by the co-administration of Ni and Cys (+13%, p < 0.001, DvsA; -13%, p < 0.001, DvsB). The activity of rat brain Na(+),K(+)-ATPase was significantly decreased by Ni-administration (-49%, p < 0.001, BvsA), while Cys supplementation could not reverse this decrease (-44%, p < 0.001, DvsA). The activity of Mg(2+)-ATPase was not affected by Ni-administration (-3%, p > 0.05, BvsA), but was significantly reduced when combined with Cys administration (-17%, p < 0.001, DvsA). The above findings suggest that Ni short-term in vivo administration causes a statistically significant decrease in the rat brain TAS and an increase in AChE activity. Both effects can be, partially or totally, reversed to control levels by Cys co-administration; Cys could thus be considered (for future applications) as a potential neuroprotective agent against chronic exposure to Ni. The activity of Na(+),K(+)-ATPase that was inhibited by Ni, could not be reversed by Cys co-administration. The matter requires further investigation in order to fully elucidate the spectrum of the neurotoxic effects of Ni.

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Section: Discussionmentioning

confidence: 93%

Short-Term Exposure to Nickel Alters the Adult Rat Brain Antioxidant Status and the Activities of Crucial Membrane-Bound Enzymes: Neuroprotection by L-Cysteine

Liapi

Ζάρρος

Theocharis

et al. 2011

Biol Trace Elem Res

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“…The results showed significant increase in the enzymatic action of Alkaline phosphatase (ALP), Alanine transaminase (ALT), Aspartate aminotransferase (AST) (Table 1, Fig. 1) in treated liver under fluoride when compare to control fish (p < 0.001) due to utilization of glucose and amino acid in carbohydrate-protein metabolism (Table 2) 10,[17][18][19][20][21] . High levels of AST, ALT, ALP upon long-term exposure (4 th week) to fluoride might be due to altered liver function or liver dysfunction under stress 22,23 .…”

Section: R E S U L T S a N D Discussionmentioning

confidence: 99%

Alteration in Hepatic Enzyme Activity of Tilapia mossambica upon Exposure to Fluoride

Aziz

Wasti

Jabeen

2018

RADS J. Biol. Res. Appl. Sci

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Fluoride, as the super reactive element fluorine, is found naturally throughout earth's crust. It has been identified as a strong, persistent powerful cumulative toxic agent, commonly distributed in the rivers, lakes, seas of earth. It is highly mobile and biologically active element in aquatic systems. Fish are considered as the excellent and valuable bioindicator of ecosystem pollution. The present study was designed to estimate acute fluoride toxicity on enzyme activity of liver of freshwater fish Tilapia mossambica. The major enzymes of Carbohydrate - Protein Metabolic pathways are Alkaline phosphatase (ALP), Alanine transaminase (ALT), Aspartate transaminase (AST) in association with carbohydrate, protein, lipid in the liver of Tilapia mossambica, from Kalri Lake (Keenjhar Lake), Sindh, Pakistan at low amount of fluoride (sub-lethal) was estimated by using UV- Visible Spectrophotometer. Results showed ALP, AST and ALT enzymes present in the liver tissue were significantly changed (p < 0.001). Finally, it is concluded that fluoride produces the adverse poisonous effect on liver functioning which may be associated with altered or elevated enzyme activity of protein-carbohydrate metabolism.

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Evaluation of toxicity of river sediments byIn Vitro enzyme inhibition

Tabata

Ozawa

Ohtakara

et al. 1990

Bull. Environ. Contam. Toxicol.

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The Effect of Intrarenal Nickel Subsulfide Injections upon the Activity of Selected Erythrocyte and Bone Marrow Enzymes in Rats

Cited by 3 publications

References 5 publications

Short-Term Exposure to Nickel Alters the Adult Rat Brain Antioxidant Status and the Activities of Crucial Membrane-Bound Enzymes: Neuroprotection by L-Cysteine

Short-Term Exposure to Nickel Alters the Adult Rat Brain Antioxidant Status and the Activities of Crucial Membrane-Bound Enzymes: Neuroprotection by L-Cysteine

Alteration in Hepatic Enzyme Activity of Tilapia mossambica upon Exposure to Fluoride

Evaluation of toxicity of river sediments byIn Vitro enzyme inhibition

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