THE EFFECT OF INBREEDING ON PHENOTYPIC VARIANCE IN THE RED FLOUR BEETLE<i>TRIBOLIUM CASTANEUM</i>

Tribolium castaneum is a small and low maintenance beetle that has emerged as a most suitable insect model for studying developmental biology and functional genetic analysis. Diverse population genetic studies have been conducted using Tribolium as the principal model to establish basic facts and principles of inbreeding experiments and response to the selection and other quantitative genetics fundamentals. The advanced molecular genetic studies presently focused on the use of Tribolium as a typical invertebrate model for higher diploid eukaryotes. After a whole genome sequencing of Tribolium, many areas of functional genomics were unraveled, which enabled the use of it in many technical approaches of genomics. The present text reviews the use of Tribolium in techniques such as RNAi, transgenic studies, immune priming, immunohistochemistry, in situ hybridization, gene sequencing for characterization of microRNAs, and gene editing using engineered endonuclease. In contrast to Drosophila, the T. castaneum holds a robust systemic RNAi response, which makes it an excellent model for comparative functional genetic studies.

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“…The negative effect of inbreeding on phenotypic variance in the T . castaneum was reported by Pray and Goodnight [ 9 ].…”

Section: Tribolium and Population Geneticsmentioning

confidence: 97%

Red flour beetle (Tribolium castaneum): From population genetics to functional genomics

et al. 2018

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“…In addition to reducing mean trait values, inbreeding might also be hypothesized to increase within-ejaculate phenotypic variance in sperm traits. Inbreeding can increase among-individual and amongpopulation variances in diverse phenotypic traits (Pray & Goodnight, 1997;Whitlock & Fowler, 1996), but might also be expected to increase within-individual variance, for example, due to reduced developmental stability. In the context of sperm performance, inbred males might produce more variable sperm, for example, due to reduced control of the spermatogenesis process (reviewed in Losdat, Chang, & Reid, 2014).…”

mentioning

confidence: 99%

No evidence of inbreeding depression in sperm performance traits in wild song sparrows

Losdat

Germain

Nietlisbach

et al. 2018

Ecology and Evolution

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Inbreeding is widely hypothesized to shape mating systems and population persistence, but such effects will depend on which traits show inbreeding depression. Population and evolutionary consequences could be substantial if inbreeding decreases sperm performance and hence decreases male fertilization success and female fertility. However, the magnitude of inbreeding depression in sperm performance traits has rarely been estimated in wild populations experiencing natural variation in inbreeding. Further, the hypothesis that inbreeding could increase within‐ejaculate variation in sperm traits and thereby further affect male fertilization success has not been explicitly tested. We used a wild pedigreed song sparrow (Melospiza melodia) population, where frequent extrapair copulations likely create strong postcopulatory competition for fertilization success, to quantify effects of male coefficient of inbreeding (f) on key sperm performance traits. We found no evidence of inbreeding depression in sperm motility, longevity, or velocity, and the within‐ejaculate variance in sperm velocity did not increase with male f. Contrary to inferences from highly inbred captive and experimental populations, our results imply that moderate inbreeding will not necessarily constrain sperm performance in wild populations. Consequently, the widely observed individual‐level and population‐level inbreeding depression in male and female fitness may not stem from reduced sperm performance in inbred males.

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Inbreeding and the genetic variance in floral traits of Mimulus guttatus

Kelly

Arathi

2003

Heredity

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The additive genetic variance, V A , is frequently used as a measure of evolutionary potential in natural plant populations. Many plants inbreed to some extent; a notable observation given that random mating is essential to the model that predicts evolutionary change from V A . With inbreeding, V A is not the only relevant component of genetic variation. Several nonadditive components emerge from the combined effects of inbreeding and genetic dominance. An important empirical question is whether these components are quantitatively significant. We use maximum likelihood estimation to extract estimates for V A and the nonadditive 'inbreeding components' from an experimental study of the wildflower Mimulus guttatus. The inbreeding components contribute significantly to four of five floral traits, including several measures of flower size and stigma-anther separation. These results indicate that inbreeding will substantially alter the evolutionary response to natural selection on floral characters.

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THE EFFECT OF INBREEDING ON PHENOTYPIC VARIANCE IN THE RED FLOUR BEETLETRIBOLIUM CASTANEUM

Cited by 11 publications

References 23 publications

Red flour beetle (Tribolium castaneum): From population genetics to functional genomics

Red flour beetle (Tribolium castaneum): From population genetics to functional genomics

No evidence of inbreeding depression in sperm performance traits in wild song sparrows

Inbreeding and the genetic variance in floral traits of Mimulus guttatus

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