The effect of IL-1β on the expression of inflammatory cytokines and their receptors in human chondrocytes

Aida, Yasuhiro; Maeno, Masao; Suzuki, Naoto; Namba, Aki; Motohashi, Masafumi; Matsumoto, Mitsuhiko; Makimura, Mizue; Matsumura, Hideo

doi:10.1016/j.lfs.2006.02.038

Cited by 53 publications

(46 citation statements)

References 33 publications

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“…The magnitudes of the compressive force used in the present study were 0.3, 0.4, 0.8, 1.6, 3.2, and 4.8 kPa, based on previous experiments 15) . The concentration of IL-1β(100 U/mL) and the experimental period of 24 h were chosen based on previous reports 12,19,20) .…”

Section: Discussionmentioning

confidence: 99%

Influence of Compressive Force and IL-1^|^beta; on Metabolism of the Extracellular Matrix in Human Chondrocytes

Nakayama

Aida

Watanabe

et al. 2012

J. Hard Tissue Biology.

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Section: Discussionmentioning

confidence: 99%

Influence of Compressive Force and IL-1^|^beta; on Metabolism of the Extracellular Matrix in Human Chondrocytes

Nakayama

Aida

Watanabe

et al. 2012

J. Hard Tissue Biology.

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“…The inflammatory cytokine interleukin-1b (IL-1b) is a common proinflammatory factor believed to be involved in osteoarthritis and intervertebral disc degeneration [2,3,[22][23][24]. IL-1b is first synthesized as an inactive precursor to pro-IL-1b, which requires cleavage of its aminoterminal region by caspase-1 to change into the secreted active form.…”

Section: Introductionmentioning

confidence: 99%

“…(2) How does the NLRP3/caspase-1/IL-1b axis in the cartilaginous endplates of patients with Modic changes compare with control (trauma) specimens? Methods Surgical specimens of cartilaginous endplates were divided into Modic changes (n = 56) and the trauma control (n = 16) groups.…”

mentioning

confidence: 99%

The NLRP3/Caspase-1/Interleukin-1β Axis Is Active in Human Lumbar Cartilaginous Endplate Degeneration

Tang

Zhu

et al. 2016

Clinical Orthopaedics &Amp; Related Research

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Background Modic changes are the MRI signal changes of degenerative lumbar vertebral endplate and which lead to or accelerate intervertebral disc degeneration. NLRP3, caspase-1, and interleukin-1b (IL-1b) play a pivotal role in the pathogenesis of many inflammatory diseases, such as osteoarthritis. However, the roles of IL-1b and its activators caspase-1 and NLRP3 are unclear in the degenerative endplate.Questions/purposes We asked: (1) What are the degenerative changes of the histologic features and chondrogenic markers' gene expressions between the cartilaginous endplates of patients with Modic changes and trauma (control)? (2) How does the NLRP3/caspase-1/IL-1b axis in the cartilaginous endplates of patients with Modic changes compare with control (trauma) specimens? Methods Surgical specimens of cartilaginous endplates were divided into Modic changes (n = 56) and the trauma control (n = 16) groups. Hematoxylin and eosin and safranin O staining of cartilaginous endplate tissues were done to evaluate the extracellular matrix. Reverse transcription-polymerase chain reaction was performed on these tissues to investigate mRNA expression of type II collagen (Col II), SOX-9, matrix metalloproteinase-3, and a disintegrin like and metalloproteinase thrombospondin type I motifs-5. NLRP3, caspase-1, and IL-1b were evaluated by reverse transcription-polymerase chain reaction and immunohistochemistry. Results Hematoxylin and eosin and safranin O staining showed the extracellular matrix degraded in the cartilaginous endplates of patients with Modic changes but not in the control cartilaginous endplates. Chondrogenic Col II (p = 0.024) and SOX9 (p = 0.053) were downregulated in the Modic changes group compared with the control group. In contrast to the control group, the transcriptional levels of

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“…The productio n o f e n d o g e n o u s I L -1 R a a p p e a r s t o b e insufficient to counteract IL-1 activity (Pritchard et al, 2008). It is also appeared that IL-1 has a significant impact on the process of damage and degradation of the cartilage in the pathogenesis of arthritis (Aida et al, 2006). Interleukin -6 (IL-6) is secreted by many cells types.…”

Section: The Short Overview Of the Role Of Cytokines In Rheumatoid Armentioning

confidence: 99%

The Stress Response and Its Functional Implications in the Immune Response After Surgery in Patients with Chronic Inflammation Undergoing Arthroplasty

Lisowska¹

2012

Recent Advances in Arthroplasty

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The effect of IL-1β on the expression of inflammatory cytokines and their receptors in human chondrocytes

Cited by 53 publications

References 33 publications

Influence of Compressive Force and IL-1^|^beta; on Metabolism of the Extracellular Matrix in Human Chondrocytes

Influence of Compressive Force and IL-1^|^beta; on Metabolism of the Extracellular Matrix in Human Chondrocytes

The NLRP3/Caspase-1/Interleukin-1β Axis Is Active in Human Lumbar Cartilaginous Endplate Degeneration

The Stress Response and Its Functional Implications in the Immune Response After Surgery in Patients with Chronic Inflammation Undergoing Arthroplasty

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