Influence of Compressive Force and IL-1^|^beta; on Metabolism of the Extracellular Matrix in Human Chondrocytes

Nakayama, Go; Aida, Yasuhiro; Watanabe, Yusuke; Honda, Kazuo; Tanigawa, Shihoko; Maeno, Masao; Matsumura, Hideo; Suzuki, Naoto

doi:10.2485/jhtb.21.217

Cited by 2 publications

(1 citation statement)

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“…Similarly, excessive and continuous cyclic mechanical stress induced load-dependent production of MMP-9 in cultured chondrocytes 11 . Similar observations have been later reported by Nakyama et al 12 . In vivo, increases in IL-6, tumor necrosis factor (TNF)-α, and MMP-9 were greater after a marathon than after a half-marathon 13 .…”

Section: Introductionsupporting

confidence: 92%

Experimental-analytical approach to assessing mechanosensitive cartilage blood marker kinetics in healthy adults: dose-response relationship and interrelationship of nine candidate markers

et al. 2021

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Purpose: To determine the suitability of selected blood biomarkers of articular cartilage as mechanosensitive markers and to investigate the dose-response relationship between ambulatory load magnitude and marker kinetics in response to load. Methods: Serum samples were collected from 24 healthy volunteers before and at three time points after a 30-minute walking stress test performed on three test days. In each experimental session, one of three ambulatory loads was applied: 100% body weight (BW); 80%BW; 120%BW. Serum concentrations of COMP, MMP-3, MMP-9, ADAMTS-4, PRG-4, CPII, C2C and IL-6 were assessed using commercial enzyme-linked immunosorbent assays. A two-stage analytical approach was used to determine the suitability of a biomarker by testing the response to the stress test (criterion I) and the dose-response relationship between ambulatory load magnitude and biomarker kinetics (criterion II). Results. COMP, MMP-3 and IL-6 at all three time points after, MMP-9 at 30 and 60 minutes after, and ADAMTS-4 and CPII at immediately after the stress test showed an average response to load or an inter-individual variation in response to load of up to 25% of pre-test levels. The relation to load magnitude on average or an inter-individual variation in this relationship was up to 8% from load level to load level. There was a positive correlation for the slopes of the change-load relationship between COMP and MMP-3, and a negative correlation for the slopes between COMP, MMP-3 and IL-6 with MMP-9, and COMP with IL6. Conclusions: COMP, MMP-3, IL-6, MMP-9, and ADAMTS-4 warrant further investigation in the context of articular cartilage mechanosensitivity and its role in joint degeneration and OA. While COMP seems to be able to reflect a rapid response, MMP-3 seems to reflect a slightly longer lasting, but probably also more distinct response. MMP-3 showed also the strongest association with the magnitude of load.

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Section: Introductionsupporting

confidence: 92%