The Effect of Gynostemma pentaphyllum Extract on Mouse Dermal Fibroblasts

Abstract

Background. The objective of this paper is to demonstrate the effect of Gynostemma pentaphyllum extract on mouse dermal fibroblasts. Recent studies have shown that this plant may possess great antioxidant properties, which can be very beneficial in combating oxidative stress. Methods. Gynostemma pentaphyllum extract was prepared and mouse dermal fibroblasts were obtained and cultured as per our laboratory protocols. Twelve samples of cells were cultured under the same conditions and both negative and positive controls were established. Induction of oxidative stress was carried out using ultraviolet C (UVC) light. Viable cell count was carried out, using microscopy. The analysis of the overall results was processed using SPSS version 16.0. Results. Statistical analysis showed strong positive correlation between the concentration of Gynostemma pentaphyllum and the mean duration of cell viability (rs = 1), with a high level of statistical significance (P < 0.01). Likewise, strong positive correlation existed between trials of cell viability (rs = 0.988–1), with statistical significance (P < 0.01). Conclusion. Gynostemma pentaphyllum extract prolongs viability of mouse dermal fibroblasts damaged by UVC light-induced oxidative stress. The results show the potential benefits of this extract on dermal cell aging.

Isolation and purification of high polar glycosides from aerial parts of Gynostemma pentaphyllum (Thunb.) Makino by linear gradient counter‐current chromatography coupled with inner‐recycling mode

“…According to Figure 2A, we first applied gradient condition I below 0-20 min, 10% A; 50 min, 20% A; 70 min, 22% A; 110 min, 24% A; 140 min, 30%A; 180 min, 36%A; 190-240 min, 60%A, with the 5.0 mL/min flow rate and 70 mg sample loading. The results showed that it was first separated into two fractions and a pure compound (5), indicating a bit strong elution capacity. Then, the gradient condition II was used to increase the peak separation efficiency, as shown below, 0-20 min, 10% A; 50-100 min, 20% A; 110-140 min, 30% A; 160-240 min, 60%A.…”

“…Makino, which belongs to the Cucurbitaceae family, has long been used in supplemental products and foods in Asian countries [1]. Gynostemma pentaphyllum is recently reported to possess various biological functions such as anticancer [2], neuroprotection [3], antidiabetes [4], antioxidation [5], immune stimulation [6], and anti‐inflammation [7]. Phytochemical investigations indicate that highly polar glycosides, including flavonoids and saponins, are the major effective ingredients related to the diversified pharmacological activities of G. pentaphyllum [8].…”

New Insights for Cellular and Molecular Mechanisms of Aging and Aging-Related Diseases: Herbal Medicine as Potential Therapeutic Approach

“…After 8 weeks of intraperitoneal injection of 100 mg/kg/d d-galactose to establish a rat model of aging with different doses of Ganoderma lucidum extract, it was found that Ganoderma lucidum could delay the progression of AD by regulating DNA methylation [207]. Lobo et al [208] gave different concentrations (0.5-5.0 mg/mL) of the Gynostemma pentaphyllum extract to mouse dermal fibroblasts, which were placed under 8-watt ultraviolet C (UVC) light at a distance of 50 cm to induce oxidative stress. The results showed that Gynostemma pentaphyllum extract prolongs viability of mouse dermal fibroblasts damaged by UVC light-induced oxidative stress, especially at 4.5 mg/mL, and it suggested that Gynostemma pentaphyllum extract had potential therapeutic effect on dermal cell aging.…”

Gypenosides Production and Spermatogenesis Recovery Potentials of Extracts from Cell Suspension Cultures of Gynostemma pentaphyllum

“…GP extract has been reported to affect cell and animal models of several diseases such as chronic liver disease 8 , dermal cell aging 9 , obesity 10 , and cancer 11 . GP has been proposed to correlate with multiple signaling pathways, including apoptosis, cell cycle arrest, inhibition of glycolysis, immunomodulatory activity, and antioxidant activity by upregulating SIRT1 and regulating the EGFR/PI3K/Akt signaling pathway 10 , 12 .…”