The effect of hypothermia on the state of the prooxidant-antioxidant system of plants

Abstract

Aim of the research: to identify changes in the value of indicators of the state of the prooxidant-antioxidant system (PAS) in the tissues of edible parts of agricultural plants under the influence of temperature changes. Methodology: Quantitative determination of indicators of the state of PAS was performed on tissue samples of edible parts of the following plants: Solánum lycopérsicum L., Сucumis sativus L., Capsicum annuum L., Solanum melongena L., Solanum tuberosum L., Allium sativum L., Allium cepa L., Daucus carota L., Beta vulgaris L., Cucurbita pepo var. Giraumontia L. The concentration of superoxide anion radical (•O2-), TBA-active products, superoxide dismutase (SOD) activity, catalase, the concentration of ascorbic acid (AA), glutathione (GSH) were determined. The results of the research show that hypothermia activates both parts of the PAS, however, cooling is accompanied by more powerful both low molecular weight and enzymatic antioxidant (AO) protection. The research of AO can be divided according to the degree of reduction of the protective value in hypothermia in the following series: SOD, catalase, GSH, AA. The most resistant in terms of changes in PAS to hypothermia is Solanum tuberosum L., Allium sativum L., Beta vulgaris L.; the least resistant is Capsicum annuum L., and Solánum lycopérsicum L. The generative organs of plants are less resistant to hypothermia than the vegetative ones. Practical consequences. As a result of the conducted biochemical analysis, it is established which method of storage of plant products is more effective in terms of preservation of AO activity: cooling or freezing.

Influence of Components of the Pro-Oxidant-Antioxidant System on the Immune Response Levels of Cereal Crop Varieties

“…The detailed step-by-step method of determining the value of all the above-described PAS indicators is given in the work "The effect of hypothermia on the prooxidant-antioxidant system's state of plants" (Bobrova et al, 2021).…”

Features of production, metabolism and significance of reactive oxygen species and products of their transformation

“…Detected in blood serum lipoprotein factor (suprol) with NADPH, which, together with iron ion and oxygen, produces superoxide anion radical [490].It is possible to add a proton to superoxide (•O 2 : -+ H + = •O-O:H). Superoxidanion radical induces and continues the FRPO chain, modifies membranes, carries out DNA single-strand breaks, protein fragmentation, is a source of other ROS, has a vasoconstrictor effect, activates ATP synthesis in mitochondria, is apparently necessary at the stage of translation of protein synthesis 3 . It is eliminated by superoxide dismutase and, to a lesser extent, ceruloplasmin and tocopherol.…”