The Effect of Hyperbaric Oxygen Therapy to the Amount of Lymphocytes in Oral Candidiasis Immunosuppressed Model

Abstract

<p><strong><em> </em></strong></p><p><strong><em>Background: </em></strong><em>Oral candidiasis generally caused by Candida albicans with prevalence of 30-50%. C. Albicans proliferation in the oral cavity was canducted by decreasing immune system of the host. Lymphocytes activation in immune system happened when there is a contact between host cell with cell wall of C. albicans as a result of C. albicans antigen. One of therapy to suppress the Candida infection sistemically is by giving hyperbaric oxygen therapy. <strong>Purpose: </strong>To determine the effects of giving hyperbaric oxygen therapy to the amount of lymphocytes in oral candidiasis immunosuppressed models. <strong>Methods:</strong> This research was post test only control group design. We used 12 wistar rats which were divided into 3 groups(n=4/3): K-(normal/ healthy wistar rats), K+(oral candidiasis immunosuppressed wistar rats), P1(oral candidiasis immunosuppressed wistar rats which were given hyperbaric oxygen therapy 5 days). K+ and P1 groups were immunosuppressed by giving dexamethasone 0,5mg/day/rat orally for 14 days, added with tetrasiklin 1 mg/day/rat. On the 3rd day immunosuppression, the rats were inducted with C.albicans smeared in the dorsum linguae once every two days for 12 days. <strong>Results:</strong> The data were analyzed with one way Anova test and showed significant difference among groups (p<0,05). To show the difference between each groups we used LSD test and showed significant difference (p<0,05) between K+ compared with P1, and K+ compared with K-. <strong>Conclusion:</strong> Hyperbaric oxygen therapy gives effect to the amount of lymphocytes in oral candidiasis immunosuppressed models.</em></p><p><em> </em><strong><em>Keywords:</em></strong><em> Oral candidiasis, immunosuppressed, hyperbaric oxygen therapy, lymphocytes</em></p><p> <strong><em>Correspondence</em></strong><strong><em>: </em></strong><em>Agni Febrina Pargaputri</em><em>, Departmen</em><em>t of</em><em> Oral </em><em>Biology </em><em>F</em><em>aculty of</em><em> </em><em>Dentistry</em><em>, Jl. Arif Rahman Hakim 150, Surabaya, Indonesia. </em><em>Phone:</em><em> 031-5945864, fax: 031-5912191, Email: </em><em><span style="text-decoration: underline;">agni.febrina</span></em><em><span style="text-decoration: underline;">@hangtuah.ac.id</span></em></p><p> </p>

Effect of hyperbaric oxygen treatment on liver hepatocyte damage in oral candidiasis immunosuppressed rats

“…It is also known as adjuvant therapy in eliminating oral candidiasis infection by C. albicans. 17 This therapy also showed has the effect of repairing liver injury. 15 In the…”

“…albicans (ATCC-10231) 6x10 8 0.1 cc was conducted on the dorsum tongue of rats, given once every two days for 12 days. 17,18 In the G3 group, the rats were given hyperbaric oxygen treatment 2.4 ATA for 30 minutes at 3 intervals of 5-minute breathing normal air. This treatment was carried out for five days continuously.…”

“…This treatment was carried out for five days continuously. 17 All rats were sacrificed at the end of the study, then the liver of all rats was taken for examining the histopathological change of hepatocytes. The number of hepatocyte damage was examined by counting the pycnotic, karyolysis, and karyorrhexis features using a light microscope with 400x magnification.…”