The effect of hormones of DNA.

Goldberg, Melvin L.; Atchley, William A.

doi:10.1073/pnas.55.4.989

Cited by 30 publications

(3 citation statements)

References 12 publications

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“…Evidence cited earlier strongly suggested that some of the plant hormones may act at the level of repression and derepression of DNA. The work of Goldberg and Atchley (1966) on the effect of mammalian hormones on the hyperchromicity of DNA correlates with the subsequent work of Valadares et al (1968) indicating that at least one of the mammalian hormones studied by is a derepressor of the DNA that codes for uterine hexokinase.…”

Section: Physiological Correlationssupporting

confidence: 64%

“…The morphological effects of these hormones have been investigated extensively, however, in spite of much work attempting to elucidate the primary site of action of these compounds, very little is yet known. Goldberg and Atchley (1966) have reported that the degree of hyperchromicity of isolated human placental deoxyribonueleic acid (DNA) can be specifically modified by the addition of appropriate mammalian hormones. Steroid, protein, and amino acid-derivative hormones at physiological concentrations were added to the placental DNA in solution.…”

Section: Introductionmentioning

confidence: 99%

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The Effect of Plant Growth Substances on the Hyperchromicity of DNA

Spang

Platt

1972

Physiologia Plantarum

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Deoxyribonucleic acid (DNA) was isolated from four day old, dark grown corn seedlings according to the procedure of Bonner for the isolation of chromatin followed by separation and purification according to Marmur. The purified DNA was dissolved in dilute saline‐citrate and the absorbance at 260 nm of the solution measured as the solution was slowly heated in a quartz cuvette. The degree of increase in absorbance of the DNA in solution as it is thermally denatured was used to assess the interaction of the DNA with plant hormones. Concentrations of 4 x 10‐5M NAA, IAA, 2,4‐D, and GA3 increased the hyperchromicity of the DNA when added to the DNA. Conversely, the same concentrations of CCC, AMO 1618, TIBA, and ABA decreased the hyperchromicity of the DNA. Kinetin, IAN, and tryptophan at 4 x 10‐5M had no measurable effect on the hyperchromicity of the DNA. Deoxyribonucleic acid from Escherichia coli and salmon sperm showed no change in hyperchromicity with added NAA at 4 x 10‐5M. The effect of these plant growth substances is most likely either on the thermally disrupted single strands or on the process in which the double strand opens up to single strands since only the high temperature portion was affected. It is postulated that if the plant growth substances act to alter the binding of the double strands of DNA in an isolated system and if this effect has a relationship to the DNA in an intact cell then this effect may be important in the control of plant growth and development.

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Section: Physiological Correlationssupporting

confidence: 64%

Section: Introductionmentioning

confidence: 99%

The Effect of Plant Growth Substances on the Hyperchromicity of DNA

Spang

Platt

1972

Physiologia Plantarum

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“…Les donnees experimentales les plus recentes tendent a prouver que les hormones vegctales et animales agissent au niveau de I'ADN et du controle de la synthese des enzymes, que les phenomenes observes ne sont done que des phenomenes secondaires resultant de cette action primaire (BONNFR 1965, CHAMPAGNAT 1969, CLEVER et KARLSON, cites par KARLSON 1962, GAI.STON et DAVIES 1969, GOLDBERG et ATCHLEY 1966. II parait ainsi difficile de mieux exprimer revolution des relations hote-parasite que par ces lignes de LEWIS (1953): «The possible combinations of Foods present plus the capacity of the parasite to respond differently to these combinations account for the varying degrees of resistance and susceptibility not explicable in other terms.…”

Section: Traitements Hormonaux Et Plantes Maladesunclassified

Quelques Aspects des Relations Hôte‐Parasite en Fonction de Traitements à l'Acide Indol‐acétique et à TAcide Gibbérellique

Volken¹

1972

Journal of Phytopathology

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Actinomycin D: Effect on induced metamorphosis in the Mexican axolotl

Prahlad

DeLanney

1967

J. Exp. Zool.

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Actinomycin D was used to inhibit the action of TS in the induction of metamorphic changes in the Mexican axolotl. AmD was administered before and after (in hours) T3 injection as follows: (1) -48; ( 2 ) -24; ( 3 ) -8; (4) 0; ( 5 ) +24; ( 6 ) +48; ( 7 ) $72; ( 8 ) +120; ( 9 ) +168. Five pg AmD administered alone was not lethal. However the majority of the animals injected with T3 and Am D died within 30 to 33 days. All experiments were at 20°C. Suppression of metamorphic change was greater in group 2 than 1 and greater in groups 4 and 5 than 6. Group 3 showed maximum inhibitory effects of AmD. Very little inhibition was seen in groups 7 through 9. Gill filament resorption and sloughing of skin could not be inhibited completely in any of the groups. Exophthalmia was seen in all except group 3. Least changes occurred in the main gill rachis and the fins. When Am D was administered to hypophysectomized axolotls eight hours prior to T3 there was complete gill filament resorption before any change was observed in controls. Also skin sloughing and exophthalmia were precocious in Am D and T3 injected hypophysectomized axol o t l~. The observations above indicate variable responses in the metamorphosing tissues of the axolotl to AmD. Hence utmost caution i s necessary in the interpretation of any of the inhibitory effects of Am D at the cellular level.This report presents evidence that SUSceptible tissues of the Mexican axolotl differ in their responses to 3,5,3'-triiodo-L-thyronine ( T3) induced metamorphosis under the inhibitory action of actinomycin D (Am D) and that in hypophysectomized animals at least one of the changes is accelerated.Recently (Prahlad and DeLanney, '65) questions were raised concerning the mode of action of T3 in the induction of metamorphic changes. Tata ('66b) has shown that an essential step in the biological action of thyroid hormone is regulation of synthesis of nuclear and cytoplasmic RNA and (Tata, '66a) that RNA and protein synthesis is required for induced regression of the tadpole tail in organ culture. He has also shown ('66b) that T3 stimulates, by 30-40% at 45 hours, the Mnei/ammonium sulphate-activated RNA polymerase whose product is DNA-like.In order to elucidate the mode of action of T3 in the induction of metamorphic changes in the neotenic axolotl it appeared logical to use actinomycin D. This antibiotic has been shown to inhibit DNAdependent RNA-polymerase activity (Reich and Goldberg, '64) by specifically binding to guanine containing sites on helical DNA J. EXP. ZOOL., 165: 425-432.and thus preventing the action of RNApolymerase. Recently such specificity has been claimed by Gellert et al. ('65), and Dingman and Sporn ('65). The only study dealing with the effect of actinomycin D on thyroid hormone induced metamorphic changes in the axolotl is that of Zalta and Beetschen ('65). Their procedure does not permit any conclusions as to the temporal relationships regarding the inhibitory action of Am D on TI induced changes. EXPERIMENTALIn this study both dark and white axo...

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The effect of hormones of DNA.

Cited by 30 publications

References 12 publications

The Effect of Plant Growth Substances on the Hyperchromicity of DNA

The Effect of Plant Growth Substances on the Hyperchromicity of DNA

Quelques Aspects des Relations Hôte‐Parasite en Fonction de Traitements à l'Acide Indol‐acétique et à TAcide Gibbérellique

Actinomycin D: Effect on induced metamorphosis in the Mexican axolotl

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