The effect of Hirudoid on random skin-flap survival in rats

Livaoğlu, Murat; Kerimoğlu, Servet; Sönmez, Bircan; Livaoğlu, Ayten; Karaçal, Naci

doi:10.1016/j.bjps.2009.03.018

Cited by 26 publications

(19 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Flap venous congestion is one of the common causes that lead to skin flap necrosis. Even though there are many studies to improve the blood circulation of the flap [1][2][3], there is no good recognized method to improve flap survival. Topical and externally applied drugs can improve blood circulation, and this method has the advantage of achieving high local drug concentration, application convenience and less side effects for the whole body, etc.…”

Section: Introductionmentioning

confidence: 99%

Investigation on the Microcirculation Effect of Local Application of Natural Hirudin on Porcine Random Skin Flap Venous Congestion

Yin

Wang

Sun

2011

Cell Biochem Biophys

View full text Add to dashboard Cite

Medicinal leech therapy is commonly used to treat venous congestion by local application to skin flaps, which are frequently employed in re-construction surgery. In the present study, we investigated the effect of natural hirudin, which was applied locally to treat the venous congestion, in random skin flaps in a Guangxi Bama miniature pig model. The congestive random skin flaps were divided into 3 groups: Group A (control group that was locally applied with saline), Group B (locally applied with 20ATU natural hirudin per each flap), Group C (locally applied with 40ATU natural hirudin per each flap). The microcirculatory changes were observed by measuring myeloperoxidase (MPO), malondialdehyde (MDA) and superoxide dismutase (SOD). The level of MPO, MDA and SOD activities in the tissue in hirudin-treated groups was significantly different compared with control group (P < 0.05), but there was no significant difference between group B and group C. At 12 days, as compared to the control group, flap survival rate in hirudin-treated groups was significantly different (P < 0.05), but there was no significant difference between the groups receiving two different doses of hirudin. Natural hirudin can improve microcirculation by inhibiting oxygen free radical damage and also improve flap survival time.

show abstract

Section: Introductionmentioning

confidence: 99%

Investigation on the Microcirculation Effect of Local Application of Natural Hirudin on Porcine Random Skin Flap Venous Congestion

Yin

Wang

Sun

2011

Cell Biochem Biophys

View full text Add to dashboard Cite

show abstract

“…In rat experiments, significant difference was detected by heparinoid use in topical insults when compared to placebo (8,9).…”

mentioning

confidence: 96%

Succesful treatment of extravasation injury with topical heparinoid in a pediatric patient

Özcan¹,

Ergül²,

Akbiyik³

et al. 2017

J Turgut Ozal Med Cent

View full text Add to dashboard Cite

“…The anticoagulation, antithrombotic, and antiplatelet aggregation effects of hirudin help VEGF to promote microvessel growth. 16 Finally, hirudin plays an anti-inflammatory role in a variety of cells. 16 It reduces free radicals and inflammatory factors in the damaged flaps, contributing to VEGF expression and angiogenesis.…”

mentioning

confidence: 99%

“…16 Finally, hirudin plays an anti-inflammatory role in a variety of cells. 16 It reduces free radicals and inflammatory factors in the damaged flaps, contributing to VEGF expression and angiogenesis. Our results showed that hirudin treatment led to an increase in VEGF expression in the congested skin flaps.…”

mentioning

confidence: 99%

Effects of Natural and Recombinant Hirudin on VEGF Expression and Random Skin Flap Survival in a Venous Congested Rat Model

Yingxin

Yin

et al. 2013

International Surgery

View full text Add to dashboard Cite

We aim to investigate the effects of locally injected natural and recombinant hirudin on vascular endothelial growth factor (VEGF) expression and flap survival in venous congested skin flaps using a rat model. A dorsal random skin flap (10 3 3 cm) was prepared on each of 30 Wistar rats to establish a venous congested model. The rats were randomly divided into 2 treatment groups [receiving subcutaneous injection of either natural hirudin (6 U) or recombinant hirudin (6 U)] and a control group, which received subcutaneous injection of physiologic saline. After treatment, skin flap survival rates were calculated. VEGF messenger RNA levels and VEGF-positive vessel density as a marker for VEGF levels were measured in the flaps during and after treatment. The skin flap VEGF messenger RNA levels increased in the natural hirudin-treated group. The VEGF-positive vessel density was increased in all 3 groups. Statistically significant increases of VEGF levels were observed in the natural and recombinant hirudin-treated groups compared with the control group (P , 0.05). The skin flap survival rates were improved in both hirudin treated groups. Natural and recombinant hirudin can increase VEGF expression in random skin flaps, which can potentially improve random skin flap survival in rats through angio genic mechanisms. Our results showed that hirudin treatment led to an increase in VEGF expression in the congested skin flaps. Natural hirudin demonstrated more pronounced effects than recombinant hirudin. Further studies are needed to understand the specific mechanisms.

show abstract

The effect of Hirudoid on random skin-flap survival in rats

Cited by 26 publications

References 24 publications

Investigation on the Microcirculation Effect of Local Application of Natural Hirudin on Porcine Random Skin Flap Venous Congestion

Investigation on the Microcirculation Effect of Local Application of Natural Hirudin on Porcine Random Skin Flap Venous Congestion

Succesful treatment of extravasation injury with topical heparinoid in a pediatric patient

Effects of Natural and Recombinant Hirudin on VEGF Expression and Random Skin Flap Survival in a Venous Congested Rat Model

Contact Info

Product

Resources

About