The Effect of High Temperature Roasted Coffea liberica on Physiological and Histological Structure of Mice’s Kidney

Abstract

Numerous varieties of coffee, such as robusta, arabica, gayo and etcetera, was made in order to fulfill the needs of its lovers. In Tungkal, Jambi, Liberica coffee was recently discovered and the chemical reactions during roasting process produces several components, which are caffeine, chlorogenic acid, monosaccharides and others, to create a unique flavor as the identity. Unique flavor of Liberica coffee is obtained from the high temperature roasting process and the components in coffee. This research aims to determine the effect of Liberica coffee (Coffea liberica), concentration (0, 8.000, 10.000 and 12.000 ppm/day) on the physiological and histological structure of the mice’s kidney (Mus musculus L.) for 28 days. The results was showed that kidney histological observation of fatty degeneration, tubular swelling, and pyknotic nucleus associated with percentages of renal tubular damage in the control group, P1, P2 and P3 were 0.71±0.96, 4.91±0.17, 5.82±0.21 and 9.01±0.13 indicating low damage (<50%) and liberica coffee does not affect physiological function, such as creatinine level.Keywords : Liberica coffee, kidney, histological

Efek Seduhan Kopi (Coffea Cenephora) Dan Teh Hitam (Camellia SinensisL) Terhadap Gambaran Histopatologis Ginjal Tikus Putih

“…Berdasarkan penelitian Widodo et al, (2019) Setelah masa adaptasi selama 7 hari hewan coba dikelompokkan menjadi 7 kelompok dan diberi perlakuan selama 30 hari. Kelompok 1 sebagai kontrol diberikan aquadest.Kelompok 2 tikus dengan perlakuan diberikan kopi 3 mg/200 gr/Hari secara per oral.…”

“…Apoptosis digunakan oleh tubuh untuk membuang sel yang mati dan diganti A B dengan sel yang baru.Apoptosis bersifat menguntungkan dan berlangsung umur hidup (Sriyanti, 2016). Sesuai dengan hasil penelitian sebelumnya yang dilakukan oleh (Widodo & Utami, 2019) menunjukkan bahwa konsumsi kopi berkafein dapat menimbulkan perubahan pada gambaran histologi seperti degenerasi lemak, pembengkakan tubular dan piknotik pada organ ginjal tikus wistar. Dan berdasarkan penelitian Jean (2010) terdapat kerusakan pada tubulus ginjal akibat pemberian teh hitam yang diamati pada ginjal mencit BALB/C seperti penutupan lumen tubulus proksimal, nekrosis sel epitel tubulus proksimal, hialin cast pada tubulus distal dan reaksi inflamasi intersisial didapatkan adanya kerusakan pada tubulus ginjal.…”