The effect of high temperature on the insecticidal properties of Bt Cotton

Chen, Dehua; Ye, Guoyou; Yang, Changqin; Yuan, Chen; Wu, Yunkang

doi:10.1016/j.envexpbot.2004.04.004

Cited by 93 publications

(80 citation statements)

References 27 publications

(20 reference statements)

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“…the boll filling stage (Chen et al, 2005). In contrast, no signif icant changes in Cry1Ac expression were observed after short episodes of stress by crop nutrition, population density, and plant growth regulator application in field studies (Adamczyk et al, 2001;Adamczyk & Meredith, 2004;Ian, 2006).…”

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confidence: 99%

“…The synthesis of Bt protein is also controlled by nitrogen metabolism (Steward, 1965). Our previous work showed that the insecticidal proteins content in leaves was highly correlated with the nitrogen metabolism of the Bt cotton (Chen et al, 2004(Chen et al, , 2005Wang et al, 2009). Therefore, investigating the relationships between the rebound characteristics of the efficacy after terminating temperature stress and the nitrogen metabolism is important for elucidating the process of the efficacy recovery.…”

mentioning

confidence: 99%

“…Chen et al / Span J Agric Res (2013) 11(2), 438-446 Thus, a large insecticidal efficacy reduction at boll period should not be expected because of rebound of the endotoxin content after the end of short-term stress (Adamczyk et al, 2001;Adamczyk & Meredith, 2004;Ian, 2006). However, extreme temperature, especially low temperature stress for longer period, can contribute to the decrease in Cry1Ac protein because of long lasting after-effect and weak recovery (Meyer & Heidmannm, 1994;Xia & Guo, 2004;Chen et al, 2005;Olsen et al, 2006). Hence, current management strategies for cotton plants at boll stage may also need to be reexamined and optimized to ensure the most efficient utilization of transgenic traits after low temperature stress caused by variable climate.…”

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confidence: 99%

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The recovery of Bt toxin content after temperature stress termination in transgenic cotton

Chen

Wen²,

Zhang

et al. 2013

Span. j. agric. res.

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The insecticidal efficacy of Bt cotton under different environments has generated controversy in recent years. The objective of this study was to investigate possible reasons of the conflicting results caused by temperature stress. Two different types of Bt transgenic cotton cultivars (a Bt cultivar, Sikang1, and an hybrid Bt cultivar, Sikang3) were selected. The plants of the two Bt cultivars were exposed to high temperature (37°C), low temperature (18°C), and the control (27°C) for short (24 h) and long (48 h) periods of stress at peak boll stage, and then moved to the glasshouse where the control plants were maintained. The results showed that the leaf insecticidal toxin content fully recovered within 24 h to the level of control after the end of short duration high-temperature treatment, and recovered mostly within 48 h of the termination of 24 h low-temperature stress. Under long duration high temperature treatment the Bt toxin content required longer recovery periods (48 and 72 h for Sikang3 and Sikang1, respectively) to reach the control level. The Bt protein content only recovered partially at 96 h after the end of the long-duration low-temperature stress, and the concentrations of the Cry1Ac protein were 74% and 77% of the corresponding control for Sikang1 and Sikang3, respectively. The different recovery and increase slowly of the leaf nitrogen metabolic rates after ceasing the heat and cold stress may be possible reason for the above difference. In summary, the duration and type of temperature (cold or heat) stress may cause different Bt insecticidal protein recovery rate due to different recovery rate of enzymes.

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The recovery of Bt toxin content after temperature stress termination in transgenic cotton

Chen

Wen²,

Zhang

et al. 2013

Span. j. agric. res.

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“…It would be interesting to investigate if pH values lower than measured in this study would significantly contribute to lower expression. A number of agronomic and environmental factors have been shown to affect the expression level of Bt proteins in plants (Trarore et al 2000;Bruns and Abel 2003;Chen et al 2005;Clark et al 2005;Stotzky 2007, 2008;Luo et al 2008;Marquardt et al 2014;Trtikova et al 2015). In maize, Marquardt et al (2014) found a positive linear relationship between nitrogen applied in the soil and Cry3Bb1 expression under greenhouse conditions.…”

Section: Discussionmentioning

confidence: 99%

Influence of calcareous soil on Cry3Bb1 expression and efficacy in the field

Wangila

Wang

et al. 2017

Transgenic Res

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Greater than expected injury by western corn rootworm (WCR) (Diabrotica virgifera virgifera LeConte) to Cry3Bb1 expressing maize hybrids (Zea mays L.) has been reported in southwestern Nebraska. Affected areas of some fields are often associated with high pH calcareous soils where maize growth is poor and iron chlorosis is common. As part of a comprehensive study to understand potential causes of unexpected injury, experiments were conducted during 2013 and 2014 to ascertain whether the calcareous soil conditions and associated poor maize growth negatively affect the expression of Cry3Bb1. Quantitative determination of Cry3Bb1 protein expression levels in root tissues was carried out on plants at V5-V6 growth stage using the enzyme-linked immunosorbent assay. Cry3Bb1 and non-Bt near isoline maize hybrids were artificially infested with Cry3Bb1-susceptible WCR eggs to measure survival and efficacy of Cry3Bb1 maize in calcareous and noncalcareous soils. Results showed that there was not a significant difference in expression of Cry3Bb1 protein between plants from calcareous and non-calcareous soils (18.9-21.2 μg/g fresh weight). Western corn rootworm survival was about sevenfold greater from the non-Bt isoline than Cry3Bb1 maize indicating that Cry3Bb1 performed as expected when infested with a Cry3Bb1 susceptible rootworm population. When survival from calcareous and non-calcareous soils was compared, no significant differences were observed in each soil. A significant positive correlation between soil pH and expression of Cry3Bb1 protein in roots was detected from samples collected in 2014 but not in 2013. No such correlation was found between soil pH and survival of WCR. Results suggest that Cry3Bb1 expression levels were sufficient to provide adequate root protection against WCR regardless of soil environment, indicating that lowered Cry3Bb1 expression is not a contributing factor to the greater than expected WCR injury observed in some southwestern Nebraska maize fields.

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“…Researchers investigating the disaster said high temperatures (37 C) were most probably responsible for causing a drop in Bt concentrations in leaves (Chen et al, 2005). Other researchers found that temperature changes, especially a cool period early on in the growing season, caused a reduction in the insect toxicity of Bt (Gunning et al 2005).…”

Section: Greenpeace Internationalmentioning

confidence: 99%

- Addressing Soil Impacts on Public Health: Issues and Recommendations

2012

Soils and Human Health

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The effect of high temperature on the insecticidal properties of Bt Cotton

Cited by 93 publications

References 27 publications

The recovery of Bt toxin content after temperature stress termination in transgenic cotton

The recovery of Bt toxin content after temperature stress termination in transgenic cotton

Influence of calcareous soil on Cry3Bb1 expression and efficacy in the field

- Addressing Soil Impacts on Public Health: Issues and Recommendations

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