The effect of high-fat diet-induced obesity on cardiovascular toxicity in wistar albino rats

Abstract

<jats:p> The consumption of a high-fat diet (HFD) is considered a risk factor for obesity development. Nonetheless, a causal role of dietary fat has never been documented, because of inadequate animal models. In our study, one group of rats was fed with standard rat diet, while other group of rats fed with high-fat diet for 4 weeks. After 4 weeks of feeding, the hemodynamic parameters in the rats fed with HFD were significantly increased as compared with control rats. Rats fed with HFD had elevated levels of serum lipids, insulin, leptin, glucose and apolipoprotein B. Lipid peroxides and caspase-3 levels were increased while serum apolipoprotein A1 and antioxidant enzymes levels in heart tissues were decreased in HFD-induced obesity in rats as compared to normal healthy control rats fed on standard rat pellet diet. This model of diet-induced obesity will be a useful tool for studying the mechanisms by which dietary fat induces the obesity in humans. </jats:p>

High Dietary Fat Selectively Increases Catalase Expression within Cardiac Mitochondria

“…Furthermore, we found that fasting elicited a similar catalase-specific response. Ad libitum consumption of high dietary fat and fasting are fundamentally different nutritional states; however, each is characterized by elevated plasma levels and oxidation of fatty acids (9,(23)(24)(25)(26). Thus, our findings are consistent with elevated circulating fatty acid levels promoting H 2 O 2 production from organelles that oxidize fatty acids.…”

“…Furthermore, these data show that 1 day of fasting also induces a quantitatively similar and selective increase in catalase expression and activity. These opposing nutritional states have in common increased levels of circulating triglycerides and increased delivery of free fatty acids to the heart (9,(23)(24)(25). It has previously been demonstrated that, in each case, this results in enhanced fatty acid oxidation (24 -26) presumably by both peroxisomes and mitochondria.…”

“…However, little has been reported on the antioxidant network response to high dietary fat, particularly in the heart. What is known is largely limited to the measurement of superoxide dismutase, glutathione peroxidase, and catalase (9,10,19,20). Given that there are a number of antioxidant enzymes residing in multiple cellular locations, and catalyzing distinct reactions, it is important that a comprehensive proteomic and temporal assessment of the antioxidant response be performed.…”

Fenugreek Seed Extract Inhibit Fat Accumulation and Ameliorates Dyslipidemia in High Fat Diet-Induced Obese Rats

“…It is reported that the levels of hepatic and cardiac markers such as ALT, AST, and LDH tend to increase in obesity [40]. The elevated levels of these enzymes in our study indicated that HFD control rats are prone to hepatic and cardiac complications.…”

Oxidized LDL Is Strictly Limited to Hyperthyroidism Irrespective of Fat Feeding in Female Sprague Dawley Rats

“…Rats initially weighing 251 ± 21 g were either assigned to normal rodent diet groups (Altromin, Germany) with 2.6 kcal/g and 11% fat or custom-designed high-fat diet (HFD) groups with 4.7 kcal/g and 56% fat (Harlan, Austria). The HFD composition was based on previous studies [23,24,25]. Food and tap water were provided ad libitum .…”