The effect of high-fat diet and 13-cis retinoic acid application on lipid profile, glycemic response and oxidative stress in female Lewis rats

Abstract

Vitamin A and its metabolites are key regulators of the development of adipose tissue and associated metabolic complications. The aim of this study was to determine the effect of high fat diet and 13-cis retinoic acid (13 cRA) application on metabolic parameters, adipogenic and inflammatory indicators in female Lewis rats. Female rats of Lewis strain were fed standard laboratory diet (STD) and high fat diet (HFD, 45% of saturated fatty acids) during 30 days. The groups were divided into additional 3 groups (6 rats each): two experimental groups that received 13 cRA orally on a daily basis during 30 days (7.5 mg/kg and 15 mg/kg, respectively) and the control group that was given sunflower oil. Animals were sacrificed after 60 days. Feeding of Lewis rats with chronic HFD diet with 13 cRA supplementation increased weight gain, adiposity index, dyslipidaemia, hyperleptinaemia, insulin resistance, VLDL concentrations, oxidative stress and atherogenic indices. Administration of 13 cRA in Lewis rats fed STD did not change the weight of the animals, but it slightly increased the atherogenic parameters. 13 cRA and HFD affect metabolic parameters, glucose and lipid metabolism in Lewis rats and its administration has a completely different effect on metabolism in rats fed STD, highlighting the complex role of vitamin A supplementation in obesity. Other factors, such as genetics, age, sex, adipose tissue distribution, also must be taken into consideration.

Beneficial Effects of Laurel (Laurus nobilis L.) and Myrtle (Myrtus communis L.) Extract on Rat Health

“…238603, shape 12 mm). Composition of standardized pellet rat feed included wheat, wheat straw, hazelnut skins, maize, soy bean dehulled, corn gluten feed, fishmeal, dicalcium phosphate, sodium chloride, whey powder, soybean oil, yeast; and contained 12% moisture, 18.5% protein, 3% fats, 6% crude fibres, 7% crude ash, E672 (vitamin A), E671 (vitamin E), E1 (Fe), E2 (I), E3 (Co), E4 (Cu), E5 (Mn), and E6 (Zn) [11,53].…”

Isotretinoin Impairs the Secretory Function of Meibomian Gland Via the PPARγ Signaling Pathway

“… 30 This concentration has also been used in other studies of isotretinoin. 31 – 33 Thirdly, we scanned the sections using whole slide scanners, and performed an overall analysis of the meibomian gland acini area. This gives a more comprehensive picture of the changes in acini area compared to localized high magnification sections in the study of Ibrahim et al 26 And last, but not least, it may also be species-related.…”

Antioxidant and Anti-Atherogenic Activities of Essential Oils from Myrtus communis L. and Laurus nobilis L. in Rat

“…The unhemolyzed serum was used for the analysis of total cholesterol (TC), triglyceride (TG), high-density lipoprotein (HDL-C) and low-density lipoprotein (LDL-C) concentrations. All lipid parameters were analyzed based on enzymatic methods as described by [ 15 , 16 , 17 , 18 ]. The liver, kidney and spleen of each rat were dissected and weighed, and tissues were used to determine oxidative and anti-oxidative status by measured the lipid peroxidation, glutathione levels, and catalase activity (CAT) as well as for antioxidative capacity analysis by methods, such as ferric reducing antioxidant power (FRAP) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) •+ free radical scavenging activity.…”

“…The unhemolyzed serum were used for the analysis of TC, TG, HDL-C and LDL-C concentrations. All lipid parameters were analyzed based on the enzymatic methods as described by [ 16 , 17 , 18 ].…”