The Effect of High-Fat Diet and Streptozotocin-Induced Diabetes and Endurance Training on Plasma Levels of Calcitonin Gene-Related Peptide and Lactate in Rats

Daneshyar, Saeed; Gharakhanlou, Reza; Nikooie, Rohollah; Forutan, Yazdan

doi:10.1016/j.jcjd.2014.03.001

Cited by 4 publications

(3 citation statements)

References 49 publications

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“…As a result, the lactate concentration in plasma is increased to the level typical of the lactate threshold induced by excessive physical activities. In this state, pyruvate oxidation in the mitochondria is significantly decreased, followed by the formation of lactate and elevated lactate plasma concentrations [ 37 , 43 – 45 ]. In morbidly obese Zucker rats, the lactate plasma concentration is increased almost 3-fold, while methyl-GBB treatment significantly improves pyruvate metabolism in mitochondria and decreases the lactate concentration almost to the control level.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Fatty Acid Metabolism Increases EPA and DHA Levels and Protects against Myocardial Ischaemia‐Reperfusion Injury in Zucker Rats

Kuka

Makrecka-Kūka

Vilks

et al. 2021

Oxidative Medicine and Cellular Longevity

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Long-chain ω-3 polyunsaturated fatty acids (PUFAs) are known to induce cardiometabolic benefits, but the metabolic pathways of their biosynthesis ensuring sufficient bioavailability require further investigation. Here, we show that a pharmacological decrease in overall fatty acid utilization promotes an increase in the levels of PUFAs and attenuates cardiometabolic disturbances in a Zucker rat metabolic syndrome model. Metabolome analysis showed that inhibition of fatty acid utilization by methyl-GBB increased the concentration of PUFAs but not the total fatty acid levels in plasma. Insulin sensitivity was improved, and the plasma insulin concentration was decreased. Overall, pharmacological modulation of fatty acid handling preserved cardiac glucose and pyruvate oxidation, protected mitochondrial functionality by decreasing long-chain acylcarnitine levels, and decreased myocardial infarct size twofold. Our work shows that partial pharmacological inhibition of fatty acid oxidation is a novel approach to selectively increase the levels of PUFAs and modulate lipid handling to prevent cardiometabolic disturbances.

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Section: Discussionmentioning

confidence: 99%

Inhibition of Fatty Acid Metabolism Increases EPA and DHA Levels and Protects against Myocardial Ischaemia‐Reperfusion Injury in Zucker Rats

Kuka

Makrecka-Kūka

Vilks

et al. 2021

Oxidative Medicine and Cellular Longevity

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show abstract

“…Plasma glucose was measured by glucose oxidase method (GOD-PAP) using a detection kit (Pars Azmun, Iran, 132504H917) and an autoanalyzer (Hitachi 902, Boehringer Manneheim, Germany) (28). Sensitivity and intra-and inter-assay coefficient of variations were 2 mg/ dL, 1.8% and 1.2%, respectively.…”

Section: Measurement Of Plasma Parametersmentioning

confidence: 99%

“…Triglyceride (TG) level was determined using an enzymatic-colorimetric method by commercial kit (Pars Azmun, Iran, 117504H917) and an autoanalyzer (Hitachi 902, Boehringer Manneheim, Germany) (28). Sensitivity and intra-and inter-assay coefficient of variations were 2 mg/dL, 1.7% and 1.5%, respectively.…”

Section: Measurement Of Plasma Parametersmentioning

confidence: 99%

The effect of aerobic exercise training on gene expression of beta3-adrenergic receptor and beta-arrestin2 in inguinal white adipose tissue of mice fed with a high fat diet

Daneshyar

Bahmani

Fourotan

2021

J Shahrekord Univ Med Sci

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Background and aims: Beta-adrenergic signaling deficiency has been established to be related to obesity and related diseases. Beta3- adrenergic receptor (Adrb3) and beta-arrestin2 (Barr2) are pivotal agents in the beta-adrenergic-signaling pathway. This study aimed to investigate the preventive effect of aerobic training on dysregulation of Adrb3 and Barr2 gene expression that was induced by high-fat diet (HFD) in inguinal white adipose tissue of mice. Materials and Methods: Twenty-one C57BL/6 mice were assigned to three groups as follows: 1) control group (n=7), 2) high-fat diet-induced overweight (HFD-OW) (n=7), and 3) high-fat diet with aerobic training (HFD-AT) (n=7). The HFD-OW group were fed with a HFD for 12 weeks. The HFD-AT group had aerobic training for six weeks on a treadmill in addition to feeding with the HFD. The real-time polymerase chain reaction (PCR) method was used to measure the gene expression of Adrb3 and Barr2 in inguinal white adipose tissue. Results: The gene expression of Adrb3 did not significantly change between groups (P>0.05). However, the expression of Barr2 in HFD-OW group was significantly increased as compared to the control group (1.5-fold: P=0.001). Interestingly, the Barr2 expression in HFD-AT group was significantly lower compared with HFD-OW group (P=0.045). Conclusion: The results indicated that aerobic training could inhibit the upregulation of Barr2 induced by HFD. It seems that a portion of the preventive effect of aerobic training on the development of obesity may be mediated by inhibiting the Barr2 expression in adipose tissue.

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S-Equol Activates cAMP Signaling at the Plasma Membrane of INS-1 Pancreatic β-Cells and Protects against Streptozotocin-Induced Hyperglycemia by Increasing β-Cell Function in Male Mice

Horiuchi¹,

Usami²,

Shirai³

et al. 2017

The Journal of Nutrition

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-equol, which is enantioselectively produced from daidzein by gut microbiota, has been suggested as a chemopreventive agent against type 2 diabetes mellitus (T2DM), but the underlying mechanisms remain unclear. We investigated the effects of -equol on pancreatic β-cell function. β-Cell growth and insulin secretion were evaluated with male Institute of Cancer Research mice and isolated pancreatic islets from the mice, respectively. The mechanisms by which -equol stimulated β-cell response were examined in INS-1 β-cells. The effect of-equol treatment on β-cell function was assessed in low-dose streptozotocin-treated mice. -equol was used at 10 μmol/L for in vitro and ex vivo studies and was administered by oral gavage (20 mg/kg, 2 times/d throughout the experimental period) for in vivo studies.-equol administration for 7 d increased Ki67-positive β-cells by 27% ( < 0.01) in mice. -equol enantioselectively enhanced glucose-stimulated insulin secretion in mouse pancreatic islets by 41% ( < 0.001). In INS-1 cells, -equol exerted stronger effects than daidzein on cell growth, insulin secretion, and cAMP-response element (CRE)-mediated transcription. These-equol effects were diminished by inhibiting protein kinase A. The effective concentration of -equol for stimulating cAMP production at the plasma membrane was lower than that for phosphodiesterase inhibition.-equol-stimulated CRE activation was negatively controlled by the knockdown of G-protein α subunit group S (stimulatory) and positively controlled by that of G-protein-coupled receptor kinase-3 and -6. Compared with vehicle-treated controls, -equol gavage treatment resulted in an increase in β-cell mass of 104% ( < 0.05), a trend toward high plasma insulin concentrations (by 118%; = 0.06), and resistance to hyperglycemia after streptozotocin treatment (78% of AUC after glucose challenge; < 0.01). -equol administration significantly increased the number of Ki67-positive proliferating β-cells by 62% ( < 0.01) and decreased that of terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling-positive apoptotic β-cells by 75% ( < 0.05). Our results show that -equol boosts β-cell function and prevents hypoglycemia in mice, suggesting its potential for T2DM prevention.

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The Effect of High-Fat Diet and Streptozotocin-Induced Diabetes and Endurance Training on Plasma Levels of Calcitonin Gene-Related Peptide and Lactate in Rats

Cited by 4 publications

References 49 publications

Inhibition of Fatty Acid Metabolism Increases EPA and DHA Levels and Protects against Myocardial Ischaemia‐Reperfusion Injury in Zucker Rats

Inhibition of Fatty Acid Metabolism Increases EPA and DHA Levels and Protects against Myocardial Ischaemia‐Reperfusion Injury in Zucker Rats

The effect of aerobic exercise training on gene expression of beta3-adrenergic receptor and beta-arrestin2 in inguinal white adipose tissue of mice fed with a high fat diet

S-Equol Activates cAMP Signaling at the Plasma Membrane of INS-1 Pancreatic β-Cells and Protects against Streptozotocin-Induced Hyperglycemia by Increasing β-Cell Function in Male Mice

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