The effect of high fat food on erythrocyte phospholipids, fatty acids composition and glutathione redox-system of rats with alimentary dyslipidemia

Abstract

To evaluate the effects of high fat food consisted of tallow (19% of total diets) and cholesterol (2%) on modification of erythrocyte phospholipids, fatty acids composition and glutathione redoxsystem of male Wistar rats with alimentary dyslipidemia. The results demonstrated that after 30 and 180 days of high-fat feed erythrocyte phosphatidylinositol and phosphatidylcholine levels were reduced, phosphatidylserine were increased. Only on the 90 days of the experiment phosphatidylinositol level increased. In all growups the erythrocyte 18:0 saturated fatty acids and 20:4n6, 22:4n6 polyunsaturated fatty acids (PUFA) were increased. Deficit of n3 PUFA-20:5n3 and 22:6n3 after 90 and 180 days high fat feed promoted compensatory synthesis from 18:1n9 on 20:3n9. Erythrocyte maleic dialdehyde increased, glutathione level decreased in all groups of rats after fed with high-fat feed. Glutathione reductase and glutathione peroxidase activity decreased in erythrocytes after 30 and 180 days of high-fat feed. In conclusion: high-fat diet during 30-90 days started adaptive answer in lipids of membrane and glutathione redox-system. Important mechanism of adaptation of a cellular membrane to high-fat diet is increase major, structuring a membrane phosphatidylethanolamine and minor, most metabolic significant fractions phospholipids (phosphatidylinositol), keeps homeostasis of 18:2n6 and 22:6n3, 20:3n9 compensatory synthesis, decrease in activity of processes lipid peroxidation, activation of enzymes of redox-system glutathione. But prolonging the high-fat feeding (180 days and more) formed failure compensatory processes (dysadaptation). It is a risk factor of developmening atherosclerosis, diabetes, steatogepatitis and other diseases.

Modification of fatty acids composition in erythrocytes lipids in arterial hypertension associated with dyslipidemia

“…Reduction ratio 20:4 n6/20: 5n3 detected in 1group patients reflected the disturbance of FA synthesis - the forerunner of eicosanoics of the different series. This state of FA metabolism can lead to increased production of oksilipins 2nd and 4th series demonstrating vasoconstrictor, aggregation properties [1,28]. …”

Changes in Hepatic Phospholipid Metabolism in Rats under UV Irradiation and Topically Treated with Cannabidiol

“…The effectiveness of this action is due to the fact that CBD exhibits much greater antioxidant activity (30–50%) than endogenous antioxidants, such as α-tocopherol or vitamin C [ 64 ]. In addition, GSH protection also improves the function of the GSH-Px-GSH system, which manifests itself in the prevention of ROS-dependent lipid peroxidation [ 65 , 66 ]. Consequently, in our studies, we show that topical application of CBD reduces lipid peroxidation, which is revealed by the decreased levels of 8-iso-PGF 2α and 4-HNE in the liver of rats exposed to UVA radiation.…”