Modification of fatty acids composition in erythrocytes lipids in arterial hypertension associated with dyslipidemia

Abstract

Background: Modification of fatty acids (FA) composition in erythrocytes lipids as an early indicator of the development of arterial hypertension (AH) and lipid disorders. Methods: We included 34 patients with arterial hypertension and 11 healthy individuals. Each patient was examined the lipid composition of serum. From erythrocytes by gas chromatography were identified fatty acids. The quantitative composition of the erythrocyte lipids FA in patients with AH presented with saturated and polyunsaturated monoenic acids with carbon chain lengths from C12 to C22. Results: In all hypertensive patients is disturbed lipid FA composition of erythrocytes. The patients with a normal level of serum lipids revealed deficiency of polyunsaturated acids n6-linoleic (18:2 n6), arachidonic (20:4 n6), dokozatetraenic 14,4% (22:4 n6). The patients with dislipidemia installed more profound restructuring of the lipid matrix of the membrane of erythrocytes. A decrease in polyunsaturated fatty acids family n3: dokozapentaenovoy (22:5 n3), docosahexaenoic PUFA (22:6 n3), the total value of n3 PUFA in 1,3 times was revealed. Conclusion: Thus, modification of erythrocyte FA are fairly subtle indicator of pathology of lipid metabolism, which manifest themselves much earlier than changes in the lipoprotein of blood plasma.

Atherosclerosis, Dyslipidemia, and Inflammation: The Significant Role of Polyunsaturated Fatty Acids

“…Hypertension (AH) and dyslipidemia (DLP) are the most important and frequent risk factors for CVD in the general population, with a prevalence of 50–80% according to different authors [75–79]. The prevalence of DLP is 40% among untreated hypertensive patients.…”

“…Modifications of erythrocyte-membrane fatty acids (FA) composition are an early indicator of the development of AH and lipid disorders [79]. Thus, modifications of erythrocyte-membrane FA are fairly subtle indicators of lipid metabolism pathology which manifest themselves much earlier than changes in plasma lipoproteins.…”

“…In particular Novgorodetseva and coworkers observed a significant reduction of 22:5n-3 and 22:6n-3 [79]. Deficiency of endogenous n-3 PUFAs may lead to changes in physicochemical properties of cell membranes, activation of the synthesis of proinflammatory and vasoconstrictive eicosanoids, and finally induction of a systemic inflammatory syndrome [84].…”

Influence of antihypertensive and metabolic drugs on fatty acids content of lipids in cardiomyocytes of rats with spontaneous hypertension

“…Як засвідчують результати інших досліджень, не менш важливу роль у роз-витку АГ відіграють зміни у складі ЖК не тільки кардіоміоцитів, а й еритроцитів та гладеньком'язових клітин судин. Одержані результати в переважній більшості мають од-накову спрямованість, відрізняючись лише за деякими окремими ЖК [16][17][18].…”

Simple Methodology for the Quantitative Analysis of Fatty Acids in Human Red Blood Cells

“…Fatty acids (FAs) are key molecules in living organisms, being involved in several metabolic functions, such as energy storage, membrane structure, signal transduction cascades and protein acylation [1]. In the past three decades, several studies have shown a correlation between the modern western diet, which is considered to be unbalanced due to its richness in n-6 polyunsaturated fatty acids (PUFA) and low levels of n-3 PUFA, and the epidemiology of some chronic diseases, namely cardiovascular diseases [2][3][4][5], diabetes [6][7][8] cell carcinoma [9], Alzheimer's disease [10] and depression [11], among others. Although some epidemiological studies performed so far to evaluate the relation between diet and diseases have relied on data from food frequency questionnaires to assess dietary intake, this approach has several limitations [12].…”