Influence of antihypertensive and metabolic drugs on fatty acids content of lipids in cardiomyocytes of rats with spontaneous hypertension

Puzyrenko, Andrii; Чекман, И. С.; Bruzgina, T.S.; Горчакова, Н. О.

doi:10.15407/ubj85.04.067

Cited by 5 publications

(4 citation statements)

References 7 publications

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“…In our study, long-chain PUFA were not associated with status of hypertension across the 10-years, except for EPA being adversely related. Increased medication use might have masked the associations, due to the interaction with lipid metabolism [31]. The large-scale Atherosclerosis Risk in Communities study previously showed protective associations between total PUFA cholesterol ester content and 6-years prevalent and incident hypertension, with individual EPA and AA exhibiting adverse associations [32].…”

Section: Discussionmentioning

confidence: 99%

Long-Chain Polyunsaturated Fatty Acids Are Associated with Blood Pressure and Hypertension over 10-Years in Black South African Adults Undergoing Nutritional Transition

Zec

Schutte

Ricci

et al. 2019

Foods

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Nutritional transition in Africa is linked with increased blood pressure (BP). We examined 10-year fatty acid status and longitudinal associations between individual long-chain polyunsaturated fatty acids (PUFA), BP and status of hypertension (≥140/90 mmHg and/or medication use) in black South Africans. We included 300 adults (>30 years) participating in the Prospective Urban Rural Epidemiology study, and analysed data from three consecutive examinations (2005, 2010 and 2015 study years). Fatty acids in plasma phospholipids were analysed by gas chromatography-mass spectrometry. We applied sequential linear mixed models for continuous outcomes and generalized mixed models for the hypertension outcome, in the complete sample and separately in urban and rural subjects. Mean baseline systolic/diastolic BP was 137/89 mmHg. Ten-year hypertension status increased among rural (48.6% to 68.6%, p = 0.001) and tended to decrease among urban subjects (67.5% to 61.9%, p = 0.253). Regardless of urbanisation, n-6 PUFA increased and eicosapentaenoic acid (EPA, C20:5 n-3) decreased over the 10-years. Subjects in the highest tertile of arachidonic acid (C20:4 n-6) had 3.81 mmHg lower systolic (95% confidence interval (CI): −7.07, −0.54) and 3.82 mmHg lower diastolic BP (DBP) (95% CI: −5.70, −1.95) compared to the reference tertile, irrespective of lifestyle and clinical confounders. Similarly, osbond acid (C22:5 n-6) was inversely associated with DBP. Over the 10-years, subjects in the highest EPA tertile presented with +2.92 and +1.94 mmHg higher SBP and DBP, respectively, and with 1.46 higher odds of being hypertensive. In black South African adults, individual plasma n-6 PUFA were inversely associated with BP, whereas EPA was adversely associated with hypertension, supporting implementation of dietary fat quality in national cardiovascular primary prevention strategies.

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Section: Discussionmentioning

confidence: 99%

Long-Chain Polyunsaturated Fatty Acids Are Associated with Blood Pressure and Hypertension over 10-Years in Black South African Adults Undergoing Nutritional Transition

Zec

Schutte

Ricci

et al. 2019

Foods

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“…Через семь дней после развития АГ уровень ТГ оставался повышенным, также происходило статистически значимое увеличение ОХ, ЛПНП, ЛПОНП и КА, то есть увеличивалось количество липидов и тех фракций липопротеидов, которые участвуют в переносе холестерина и ТГ от печени к периферическим тканям [11,25]. Уровень ЛПВП повышался, однако по соотношению с другими фракциями это повышение было несущественным.…”

Section: результаты и их обсуждениеunclassified

Lipid and Glucose Levels in the Rats Blood Serum Under the Stress-Induced Hypertension and After the Administration of Cryopreserved Cord Blood

Babiychuk

Sirotenko

Malova

et al. 2014

PEP

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It was shown that in young and old male rats against the background of experimentally inducedhypertension were signiﬁcantly increased levels of triglycerides, total cholesterol, low density andvery low density lipoproteids, which conﬁrms the presence and progression of this disease in animals.Injection of cryopreserved nucleated cells of cord blood to hypertensive animals is accompanied bynormalization of the lipid proﬁle, decrease of atherogenic index. The experimental data indicate thepossibility of correction of lipid proﬁle of animals with hypertension on account of the inﬂuence ofanti-atherogenic properties of cryopreserved preparation of cord blood.

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“…Energy supply to the adult heart mainly relies on fatty acids; abnormal fatty acid metabolism is often accompanied with heart disease or systemic disease (5,6). It has been reported that acute myocardial ischemia, diabetes, and obesity patients have an increased risk of abnormal deposition of myocardial fat, leading to heart failure (7). Palmitate (PAL), a saturated fatty acid, has been shown to accumulate in cardiomyocytes, vascular smooth muscle cells, hepatic cells, and islet beta cells (8)(9)(10)(11)(12).…”

Section: Introductionmentioning

confidence: 99%

Vascular Endothelial Growth Factor Ameliorated Palmitate Induced Cardiomyocyte Injury via JNK Pathway

Wang

Zou

Liu³

et al. 2020

Preprint

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Objective To investigate the effect of palmitate (PAL) on apoptosis and the timing and activity of VEGF expression in HHHM2 myocardial cells (a human embryonic cardiomyocyte cell- line). Methods 1. Cardiomyocytes were divided into the following five groups: the control group and the 0.2 mM, 0.5 mM, 0.8 mM, and 1.2 mM PAL groups. We examined the changes in cell viability by MTT assay after PAL incubation for 24 h and the cardiomyocyte apoptosis rate by FACS examination, and thus determined the effective concentration of PAL. The transcription levels of CASP3, Bcl-2, Bax, and VEGF were detected by quantitative fluorescence PCR and the protein expression of caspase 3 and VEGF by western blot. 2. To observe the time-dependent effects on cell injury induced by 0.5 mM PAL, cardiomyocytes were divided into 0, 4, 8, 16, 24, and 48 h groups. The variation in cell viability was examined by MTT assay. The transcription levels of CASP3, Bcl-2, Bax, and VEGF were detected by quantitative fluorescence PCR and the protein expression of caspase 3 and VEGF by western blot. 3. To observe the effects of VEGF on the PAL induced apoptosis of cardiomyocytes, the cells were divided into the control group and the VEGF overexpression group. At 24 h after transfection, cells were incubated with 0.5 mM PAL for 6, 12, 24, and 48 h. Cell viability was examined by MTT assay. The apoptosis rate was measured by FACS using the Annexin V-FITC kit. The transcription levels of CASP3, Bcl-2, Bax, NF-kB p65, and VEGF were measured by quantitative fluorescence PCR, the protein expression of VEGF, caspase 3, Bcl-2, Bax, NF-κB p65, p-JNK/JNK, and p-ERK/ERK were measured by western blot, as well as caspase 3 activity. Results 1. A dose-dependent relation between the concentration of PAL and H9c2 cardiomyocyte injury was observed. In the 0.5 mM group, the apoptosis rate was increased significantly, while cell viability was decreased, indicating that 0.5 mM PAL was the ideal concentration to induce cardiomyocyte apoptosis. The expression of caspase 3 and Bax was significantly increased, and the expression of VEGF was enhanced, while the levels of Bcl-2 remained unchanged during the process. 2. A significant time-dependent relation between PAL and cardiomyocyte injury was observed. The apoptosis rate was increased greatly after 16 h treatment with 0.5 mM PAL. 3. Cell viability was restored by VEGF overexpression during treatment with 0.5 mM PAL. The apoptosis rate was also reduced by VEGF overexpression, as detected by FACS. The expression of caspase 3, Bax, and NF-κB p65 was significantly decreased, Bcl-2 and VEGF expression was dramatically increased, p-JNK/JNK expression was significantly enhanced, p-ERK/ERK levels did not exhibit a significant change, and the activity of caspase 3 was significantly decreased. Conclusions 1. PAL can induce injury and apoptosis in HHHM2 myocardial cells, and these effects are time-dependent. A PAL concentration of 0.5 mM was ideal to establish the cardiac cell injury model. 2. PAL at a concentration of 0.5 mM can effectively induce cardiomyocyte injury and enhance the expression of caspase 3, Bax, and VEGF, especially after 24 h and 48 h of PAL treatment. 3. VEGF overexpression can reverse the effects of PAL on apoptosis and cell viability. In addition, VEGF overexpression inhibited the expression of proapoptotic and inflammatory factors, caspase 3 activity, and transduction of the MAPK signaling pathway.

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Influence of antihypertensive and metabolic drugs on fatty acids content of lipids in cardiomyocytes of rats with spontaneous hypertension

Cited by 5 publications

References 7 publications

Long-Chain Polyunsaturated Fatty Acids Are Associated with Blood Pressure and Hypertension over 10-Years in Black South African Adults Undergoing Nutritional Transition

Long-Chain Polyunsaturated Fatty Acids Are Associated with Blood Pressure and Hypertension over 10-Years in Black South African Adults Undergoing Nutritional Transition

Lipid and Glucose Levels in the Rats Blood Serum Under the Stress-Induced Hypertension and After the Administration of Cryopreserved Cord Blood

Vascular Endothelial Growth Factor Ameliorated Palmitate Induced Cardiomyocyte Injury via JNK Pathway

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