The effect of heparin concentration on certain blood parameters of the rainbow trout Salmo gairdneri Richardson

Barham, W.T.; Smit, G. L.; Schoonbee, H. J.

doi:10.1016/0306-4492(79)90088-1

Cited by 8 publications

(3 citation statements)

References 11 publications

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“…(1973) although the glucose component was omitted. The use of anticoagulants in the cannulae was avoided due to their reported influence upon lipolytic activity in higher vertebrates (Newsholme & Start, 1973) and the blood chemistry of fish (Smit e f al., 1977;Barnham et al, 1979).…”

Section: Methodsmentioning

confidence: 99%

Plasma glucose and plasma fatty acid levels of Limanda limanda (L.) in relation to season, stress, glucose loads and nutritional state

Fletcher¹

1984

Journal of Fish Biology

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The effects of season, stress, glucose loads and nutritional state on the plasma glucose (PG) and plasma fatty acids (PFA) of Limanda limandu were examined. Plasma glucose levels showed no seasonal variation while PFA exhibited two peaks during May to August, and in December respectively. These peaks may be associated with resorption of gonadal material after spawning and the mobilization of lipid reserves for vitellogenesis. Lirnandu showed a hyperglycaemic response to a brief aerial emersion, followed by a hypoglycaemic condition. Infusion of a glucose load and the ingestion of a satiation meal ofA4ytilu.s edulis tissue led to a reciprocal rise and fall of PG and PFA. Short-term food deprivation had no effect upon PG although PFA rose significantly.

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Section: Methodsmentioning

confidence: 99%

Plasma glucose and plasma fatty acid levels of Limanda limanda (L.) in relation to season, stress, glucose loads and nutritional state

Fletcher¹

1984

Journal of Fish Biology

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“…Blood sampling was done using 2-ml one-way plastic syringes and size 1 one-way canules (Braun AG). To avoid clotting of the blood and to ensure anaerobic blood sampling, the dead volume of the syringes was filled with 123 pi sodium heparine solution so that a sample of 1 ml of blood contained a concentration of 3.4 mg sodium heparine (= 525 USP-U, 150 USP-U/mg, Serva GmbH) (BARHAM et al 1979;SMIT and H A~I N G H 1980). Air bubbles were immediately squeezed out.…”

Section: Methodsmentioning

confidence: 99%

Relationships among carcass weight, acid-base-balance of the blood, meat quality parameters and body composition of cultured rainbow trout (Oncorhynchus mykiss)

Schmelzing¹,

Claus

1994

J Appl Ichthyol

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The relationshi s among carcass weight, acid-base-balance of the blood as an indicator for metabolic load susceptibif&, meat quality parameters, and body Composition were investigated in rainbow trout. Examinations were carried out in two replicates using a total of 300 trout from a Danish population. Within replicates, the trout were of ap roximately the same age. In Replicate I trout, increasing carcass weight was accompanied by a more arkaline reaction of the blood. Carcass weight was significantly correlated with blood-pH and bicarbonate concentration (.19 and .26). Regression coefficients, however, confirmed only an increase of bicarbonate concentration with increasing carcass weight (b = .013 mmol/L per g, sb = 0.005). In meat quality parameters, muscular pH14 values decreased linearly in both replicates with increasing carcass weight, as shown b significant correlation estimates (-20 and -.27) and regression coefficients (b = -.0006, sb = .0002, a n d i = -.001, sb = .0003). Relationshi s of other meat quality parameters to inmeasin5 carcass wei ht were evaluated differently by corriation and regression coefficients. No relationship was found %etween carcass weight and body composition. In general, correlation estimates describing interrelations between acid-base and meat quality arameters were on a very low level. Independent from carcass weight, there were clearly metabolically!oaded and unloaded trout as well as trout with a mixed acid-base-status. Acid-base parameters were significantly correlated with each other. Generally, estimates for correlations between meat quality parameters were on a very low level. Body composition arameters, dry weight and crude fat content were highly significantly correlated (0.47) in Replicate h; in Replicate I there was no interrelation.

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“…To minimize the effect of handling stress, fish were held overnight in aquaria. Anaesthetization and blood sampling were performed as outlined by Barham et al (19796), the sample size for each group was ten. The method outlined by Dacie & Lewis (1975) was employed to establish osmotic fragilities of blood samples obtained from each group of fish.…”

Section: Methodsmentioning

confidence: 99%

The effect of bacterial infection on erythrocyte fragility and sedimentation rate of rainbow trout, Salmo gairdneri Richardson

Barham

Smit

Schoonbee

1980

Journal of Fish Biology

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Osmotic fragilities and erythrocyte sedimentation rates (ESR) were measured in healthy, diseased, treated and recovered trout, Salmo gairdneri. Bacterial infection with Aeromonas and Streptococcus resulted in more fragile erythrocytes and raised ESR values. Treatment with antibacterial agents improved the values of both parameters. Possible reasons for such changes are discussed. Caution in the use of apparently healthy fish for the unqualified interpretation of haematological results is expressed.

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The effect of heparin concentration on certain blood parameters of the rainbow trout Salmo gairdneri Richardson

Cited by 8 publications

References 11 publications

Plasma glucose and plasma fatty acid levels of Limanda limanda (L.) in relation to season, stress, glucose loads and nutritional state

Plasma glucose and plasma fatty acid levels of Limanda limanda (L.) in relation to season, stress, glucose loads and nutritional state

Relationships among carcass weight, acid-base-balance of the blood, meat quality parameters and body composition of cultured rainbow trout (Oncorhynchus mykiss)

The effect of bacterial infection on erythrocyte fragility and sedimentation rate of rainbow trout, Salmo gairdneri Richardson

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