The effect of heat stress onGHR,IGF-I,ANT,UCPandCOXIIImRNA expression in the liver and muscle of high and low feed efficiency female quail

Abstract

1. A study was conducted to test the hypothesis that feed efficiency (FE) correlated with the expression of genes from the somatotropic axis and mitochondrial genes involved in energy production, and that the environment to which the birds are exposed influenced the expression of such genes. 2. Quails were divided into High-FE and low-FE groups and maintained in a comfortable or heat stress (HS) (38°C for 24 h) environment to evaluate changes in insulin-like growth factor I (IGF-I), growth hormone receptor (GHR), adenine nucleotide translocator (ANT), uncoupling protein (UCP) and cytochrome oxidase subunit III (COX III) messenger ribonucleic acid (mRNA) expression in liver and muscle tissues. 3. High-FE quails (0.28 g/g) presented a higher final body weight, greater weight gain and a better feed conversion ratio than low-FE birds (0.18 g/g). High-FE birds showed greater IGF-I mRNA expression in the liver and muscle and greater GHR mRNA expression in the muscle. 4. Environmental effects only affected GHR expression in the liver, with quails under comfortable conditions exhibiting greater GHR expression than quails subjected to HS. 5. There was a significant interaction between FE and environmental temperature on ANT mRNA expression in the liver. The greatest ANT mRNA expression was observed for high FE-birds that remained under comfortable conditions. 6. In the liver, UCP mRNA expression did not differ among the quails and was not affected by environment or efficiency. However, comparisons of the low- and high-FE birds revealed higher levels of UCP mRNA in the muscle of low-FE birds. 7. COX III mRNA expression in the liver was dependent on environmental temperature and FE. Higher COX III mRNA expression was observed in animals that remained under comfortable conditions, and high-FE birds exhibited higher expression levels compared to low-FE birds. 8. These results suggest a correlation between IGF-I, GHR, ANT, UCP and COX III gene expression and FE and that environmental temperature could affect the expression of some of these genes.

mRNA expression of ANT genes, productive performance and nitrogen excretion of laying hen by feeding low protein diets and amino acid supplementation

“…According to the results shown in Fig 1, the expression of ANT gene in the breast muscle of laying hen was significantly higher in the low-CP + AA group when compared with other groups (P<0.01), while it did not differ significantly between high-CP and low-CP groups. Botteje and Carstens (2009) found that AA supplementation to low protein diets improved the FE in broilers, which has also been reported in quails (Gasparino et al (2014). Our study has also shown that amino acid supplementation resulted in a better growth performance of laying hens, affected the expression of the ANT gene and altered the peak FE.…”

Characterization of the acute heat stress response in gilts: III. Genome-wide association studies of thermotolerance traits in pigs

“…GHR mRNA abundance in hepatic tissue of lactating Holstein dairy cows (Deane and Woo, 2005;Rhoads et al, 2010) and avian species (Gasparino et al, 2014;Del Vesco et al, 2015), and is independent of the heat-induced feed intake reduction (Collier et al, 2008). Additionally, although not always observed (Rhoads et al, 2009), circulating GH levels decline in HS compared to TN cattle (Farooq et al, 2010); this decrease in circulating GH is attributed to reduced GH secretion at the pituitary gland.…”

Comparative assessment of growth performance of three different indigenous goat breeds exposed to summer heat stress

“…The IGF-1 is an endocrine, autocrine and paracrine growth factor that plays a major role in the cell growth, development and differentiation (Delafontaine, Song, & Li, 2004). Significantly reduced PBMC IGF-1 mRNA expression in OHS group could be attributed to the reduction in protein synthesis and higher level of protein breakdown in these animals (Gasparino et al, 2014). Lower rates of IGF-1 mRNA expression in OHS animals indicate the compromised growth performance in Osmanabadi breed.…”