The effect of heat stress on intestinal integrity and Salmonella invasion in broiler birds

Alhenaky, Alhanof; Abdelqader, Anas; Abuajamieh, Mohannad; Al-Fataftah, Abdur-Rahman

doi:10.1016/j.jtherbio.2017.10.015

Cited by 121 publications

(99 citation statements)

References 22 publications

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“…Decreased ACTH may be related to the feedback mechanisms. Numbers studies have reported that HS can disrupt intestinal mucosal tight junction, increase intestinal permeability and elevate the risk for infections of pathogens (Pearce et al, 2012;Pearce et al, 2013;Pearce et al, 2014;Pearce et al, 2015;Alhenaky et al 2017), which can active the immune system. Song et al (2019) observed the increased of IgA and IgM in serum of HS rats.…”

Section: Discussionmentioning

confidence: 99%

Effects of acute heat stress on intestinal microbiota in grow‐finishing pigs, and associations with feed intake and serum profile

Xiong

et al. 2019

J Appl Microbiol

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Aims This study was conducted to assess the effects of acute heat stress (HS) on intestinal microbiota, and the associations with the changes in feed intake (FI) and serum profile. Methods and Results Twenty four individually housed pigs (Duroc × Large White × Landrace, 30 ± 1 kg body weight) were randomly assigned to receive one of three treatments (8 pigs/treatment): (i) thermal neutral (TN) conditions (25 ± 1°C), (ii) HS conditions (35 ± 1°C), (iii) pair‐feeding (PF) with HS under TN conditions. After 24‐h treatment, pigs were monitored to assess FI, and samples of serum and faeces were collected to investigate serum profile, microbial composition and short chain fatty acids (SCFAs). The results showed that HS decreased (P < 0·05) FI compared with the TN group. Compared with TN group, HS changed the serum profile by affecting biochemical parameters and hormones related with energy metabolism and stress response; immune indicators were also altered in HS group. Most of changes in serum profile were independent of FI reduction. Additionally, HS shifted the diversity and composition of faecal microbial community by increasing (P < 0·05) Proteobacteria and decreasing (P < 0·05) Bacteroidetes. Moreover, HS decreased (P < 0·05) the concentrations of propionate, butyrate, valerate, iso‐valerate and total SCFAs in faeces in an FI‐independent manner. Furthermore, the Spearman correlation analysis implied that changes of serum profile have potential correlation with alterations of faecal microbiota and their SCFAs metabolites in acute HS‐treated grow‐finishing pigs. Conclusions Metabolism disorders caused by 24‐h acute HS associated with changes of faecal microbiota and their SCFAs metabolites in an FI‐independent manner in grow‐finishing pigs. Significance and Impact of the Study These results give us a new insight of the intestinal damage caused by acute HS and the underlying mechanisms.

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Section: Discussionmentioning

confidence: 99%

Effects of acute heat stress on intestinal microbiota in grow‐finishing pigs, and associations with feed intake and serum profile

Xiong

et al. 2019

J Appl Microbiol

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“…High ambient temperature is one of the most serious environmental factors that adversely affect animal production performance and health ( Nardone et al., 2010 , Kamel et al., 2017 ), and it is responsible for heavy economic losses and has become a significant challenge in animal husbandry industry ( St-Pierre et al., 2003 , Nawab et al., 2018 ). Environmental stressors may also increase the risk of colonization with pathogens such as Salmonella and Campylobacter in the gut of animals, and some pathogens can disseminate to other animals as well as to humans, that is, it has the potential to alter coexistence in host–pathogen systems and may pose a serious threat to public health ( Freestone et al., 2008 , Verbrugghe et al., 2012 , Lara and Rostagno, 2013 , Alhenaky et al., 2017 ).…”

Section: Introductionmentioning

confidence: 99%

High-temperature exposure alters the community structure and functional features of the intestinal microbiota in Shaoxing ducks (Anas platyrhynchos)

Tian

Chen

et al. 2020

Poultry Science

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The gut microbiome is a complex ecosystem that contributes to host nutrition and health. However, our current knowledge of the relationship between ambient temperature and gut microbiota of poultry is still limited. The objective of the present study was to characterize the intestinal microbiota of ducks exposed to high ambient temperature. Sixty 60-day-old Shaoxing ducks were allocated to control and heat-treated groups. The ducks in the control group were kept at 25°C, and the ducks in the heat treatment group were raised at 30–40°C, which simulated the temperature change of day and night in summer. After 15 D, the intestinal contents of the duodenum, jejunum, and ileum were obtained from 6 ducks of each group. Genomic DNA was extracted and amplified based on the V4–V5 hypervariable region of 16S rRNA. The results showed that Firmicutes was the dominant bacterial phylum with the highest abundance in the contents of the small intestine of ducks, and the relative abundance of the phylum Firmicutes in all 3 intestinal segments was increased by high temperature. At the genus level, Lactobacillus was found to be the most dominant bacterial genus across 3 gut segments, and its abundance was increased in ducks under heat treatment. Compared with the corresponding intestine segment of control ducks, a total of 36 genera in the duodenum, 19 genera in the jejunum, and 6 genera in the ileum of heat-treated ducks were found to be significantly different in the abundance (linear discriminant analysis score >3.0, P < 0.05). Functional prediction of gut microbiota revealed that high temperature caused changes in the abundance of metabolism and transcription-related pathways. It is noteworthy that most of the altered pathways are related to metabolism. In conclusion, high temperature induced remarkable taxonomic changes in the gut microbiome of ducks, which might be related to the negative effects of high temperature in ducks. Our present study provided an important theoretical ground for high-temperature intervention.

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“…These small molecules proteins are released when the animal is exposed to infection, inflammation and shock as an immune response [77]. Heat stress affects intestinal integrity and increases intestinal permeability to endotoxin and antigens and inflammatory cytokines [78]. Heat stress has been shown to increase the expression of pro-inflammatory cytokines and suppressed anti-inflammatory cytokines in broilers [79].…”

Section: Cytokines Mrna Expressionmentioning

confidence: 99%

Supplementation of Postbiotic RI11 Improves Antioxidant Enzymes Activity, Upregulated Gut Barrier Genes and Reduced Cytokines, Acute Phase Proteins and HSP70 Gene Expression Levels in Heat-Stressed Broilers

Humam

Loh

Foo

et al. 2020

Preprint

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Background To alleviate the adverse impacts of stressful environmental conditions on poultry and promoting the animal's health and growth performance, antibiotics have been added to poultry diets as growth promoters. Nevertheless, improper and overuse of antibiotics as feed additives have resulted the emergence of antibiotic-resistant bacteria and increased the levels of antibiotic residues in animal products, which have disastrous effects on the health of both animals and humans. Postbiotics produced from probiotic Lactobacillus plantarum have been the recent research of interest as dietary additives for livestock and potential alternatives to antibiotics. However, there is very scarce of study has considered the effect of postbiotics on broilers under heat stress. The aim of this work was to evaluate the impacts of feeding different levels of postbiotic RI11 on antioxidant enzyme activity, physiological stress indicators, cytokines and gut barrier genes expression in broilers under heat stress. Materials and Methods A total of 252 male Cobb 500 were fed with 1 of 7 diets: NC (negative control, 0.0% RI11) basal diet; OTC (positive control) NC + 0.02% (w/w) oxytetracycline; AA (antioxidant control) NC + 0.02% (w/w) ascorbic acid. Four further groups were NC + 0.2, 0.4, 0.6 and 0.8% postbiotic RI11 (v/w) of the respective levels. Results Supplementation of different levels of postbiotic RI11 increased plasma glutathione peroxidase, catalase and glutathione enzymes activity. Postbiotic RI11 groups upregulated the mRNA expression of interleukin 10 and downregulated of interleukin 8, tumor necrosis factor-alpha, heat shock protein 70 and alpha 1- acid glycoprotein levels compared to the NC and OTC groups. Feeding various doges of postbiotic RI11 improved the integrity of the intestinal barrier by the upregulation of zonula occludens-1 and mucin2 mRNA expressions. However, no difference was observed in claudin1, ceruloplasmin, interleukin 6, interleukin 2 and interferon expression, but downregulation for occludin expression as compared with the NC group. Supplementation of postbiotic RI11 in different levels quadratically increased the plasma glutathione peroxidase, catalase and glutathione, interleukin 10, mucin2 and zonula occludens-1 mRNA expression, and reduced plasma interleukin 8, tumor necrosis factor-alpha, alpha 1- acid glycoprotein and heat shock protein 70 mRNA expression. Supplementation of postbiotic RI11 at level 0.6% was sufficient to achieve the improvement in health of broiler chickens under heat stress as compared to other levels. Conclusions The results suggested that postbiotic produced from L. plantarum RI11 particularly at level 0.6% (v/w) could be used as an alternative to antibiotics and natural sources of antioxidants in the poultry industry.

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The effect of heat stress on intestinal integrity and Salmonella invasion in broiler birds

Cited by 121 publications

References 22 publications

Effects of acute heat stress on intestinal microbiota in grow‐finishing pigs, and associations with feed intake and serum profile

Effects of acute heat stress on intestinal microbiota in grow‐finishing pigs, and associations with feed intake and serum profile

High-temperature exposure alters the community structure and functional features of the intestinal microbiota in Shaoxing ducks (Anas platyrhynchos)

Supplementation of Postbiotic RI11 Improves Antioxidant Enzymes Activity, Upregulated Gut Barrier Genes and Reduced Cytokines, Acute Phase Proteins and HSP70 Gene Expression Levels in Heat-Stressed Broilers

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