The effect of growth temperature, on big-vein symptom and serological detection of Mirafiori lettuce virus and Lettuce big-vein virus in lettuce.

Maekawa, Kazumasa; Sasaya, Takahide; Fujii, Hiroki; Ishikawa, Koichi; Kanto, Takeshi; Iwamoto, Yutaka; Aino, Masataka

doi:10.3186/jjphytopath.70.320

Cited by 6 publications

(3 citation statements)

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“…LBVaV (18/20) in the inoculated lettuce plants compared to MiLBVV (6/20) can be attributed to the fact that LBVaV infection develops faster than MiLBVV infection, and that LBVaV replicated over a wider range of temperatures than MiLBVV as previously reported byMaekawa et al (2004). Recently, Bernal-Vicente et al(2018)showed that LBVaV accumulated to higher levels in contrast to MiLBVV in different types of plant tissues (leaves, roots, symptomatic, asymptomatic).…”

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confidence: 74%

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Occurrence and partial genetic characterisation of Lettuce big‐vein associated virus and Mirafiori lettuce big‐vein virus infecting lettuce in Jordan

Salem

Odeh

Muslem

et al. 2020

Annals of Applied Biology

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Lettuce big-vein disease (LBVD) is an economically damaging disease that occurs in most lettuce production areas worldwide. Several studies have associated LBVD with a complex of two viruses, Lettuce big-vein associated virus (LBVaV) and Mirafiori lettuce big-vein virus (MiLBVV). Both viruses are transmitted through soil by the zoospores of the chytridiomycete fungus Olpidium virulentus. During surveys carried out in two growing seasons from 2015 to 2016 in the main vegetable growing areas in Jordan (Jordan Valley, Jerash, and Madaba), 179 lettuce plants, 46 lettuce seedlings, and 56 weed plants were collected. RNA extraction was conducted from leaves of all samples. Associated viruses were identified using reverse transcription-polymerase chain reaction (RT-PCR) with specific primer pairs targeting part of the coat protein (CP) genes of LBVaV and MiLBVV. RT-PCR results revealed that, out of 225 lettuce samples tested, 32 (14%) and 44 (20%) were singly infected with LBVaV and MiLBVV, respectively, and 73 (32%) samples were dually infected with LBVaV and MiLBVV. Overall, MiLBVV was detected in more lettuce samples (117) compared with LBVaV which was detected in 105 samples. Moreover, one sample of each weed species of Chenopodium murale and Sonchus oleraceus tested positive for MiLBVV and LBVaV, respectively. The partial nucleotide sequence of the CP genes of LBVaV and MiLBVV Jordanian isolates showed similarities to other isolates available in the NCBI database ranging between 93.9-100% and 88.3-100%, respectively. Nucleotide sequence comparison of the Jordanian isolates and inference from phylogenetic trees with strong support revealed well-defined subgroups with little or no correlation with planting area or geographic origin. The occurrence of both viruses in lettuce plants was associated with the presence of resting spores and zoospores of Olpidium spp. in lettuce plants grown under field and growth chamber conditions. Lettuce plants inoculated with Olpidium spp. zoospores isolated from roots of symptomatic lettuce plants, developed LBVD-symptoms. At 60 days post-transplantation, both viruses were detected by RT-PCR in most of the plants analysed. Biological and molecular assays together with the light microscopy observations of resting spores and

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confidence: 74%

“…The present investigation examined the prevalence and genetic diver- intensities (Lot et al, 2002;Walsh, 1994;Westerlund, Campbell, & Grogan, 1978). Maekawa et al (2004) demonstrated that a decrease in growth temperature from 27 C to 18 C facilitated the appearance of big-vein symptoms and the detection of MiLBVV in the leaves.…”

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confidence: 96%

Occurrence and partial genetic characterisation of Lettuce big‐vein associated virus and Mirafiori lettuce big‐vein virus infecting lettuce in Jordan

Salem

Odeh

Muslem

et al. 2020

Annals of Applied Biology

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“…Segundo Walsh (25), plantas submetidas a uma temperatura constante de 18°C desenvolvem sintomas mais intensos e mais rapidamente, assim como plantas que recebem luz de baixa intensidade durante um período longo, de 18 horas, podendo este fato estar associado com a redução da produção de componentes celulares pela planta. Maekawa et al (13) …”

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Frequência de Lettuce big-vein associated virus e Mirafiori lettuce big-vein virus em Plantas de alface no Estado de São Paulo e transmissão através de extrato vegetal e solo

et al. 2007

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Molecular analysis of coat protein coding region of tobacco stunt virus shows that it is a strain of Lettuce big-vein virus in the genus Varicosavirus

et al. 2005

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To evaluate the relationship between tobacco stunt virus (TStV) and Lettuce big-vein virus (LBVV), we determined nucleotide sequences of the coat protein (CP) coding region of five TStV and three LBVV isolates and compared them with those of one Japanese and four Spanish isolates of LBVV. CP coding regions were identical in size and the nucleotide and amino acid sequence identities between TStV and LBVV were 95.6-96.5% and 97.2-98.7%, respectively. Phylogenetic analysis of nucleotide sequences indicated that TStV was very closely related to LBVV and a strain of LBVV rather than a distinct species.

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The effect of growth temperature, on big-vein symptom and serological detection of Mirafiori lettuce virus and Lettuce big-vein virus in lettuce.

Cited by 6 publications

References 5 publications

Occurrence and partial genetic characterisation of Lettuce big‐vein associated virus and Mirafiori lettuce big‐vein virus infecting lettuce in Jordan

Occurrence and partial genetic characterisation of Lettuce big‐vein associated virus and Mirafiori lettuce big‐vein virus infecting lettuce in Jordan

Frequência de Lettuce big-vein associated virus e Mirafiori lettuce big-vein virus em Plantas de alface no Estado de São Paulo e transmissão através de extrato vegetal e solo

Molecular analysis of coat protein coding region of tobacco stunt virus shows that it is a strain of Lettuce big-vein virus in the genus Varicosavirus

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