The effect of growth phase on the surface properties of three oleaginous microalgae (Botryococcus sp. FACGB-762, Chlorella sp. XJ-445 and Desmodesmus bijugatus XJ-231)

Xia, Ling; Huang, Rong; Li, Yinta; Song, Shaoxian

doi:10.1371/journal.pone.0186434

Cited by 37 publications

(9 citation statements)

References 30 publications

(41 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…EPS matrix produced by microorganisms promotes the cellular organization into microbial communities (biofilms), increases the capacity of biofilm formers to trap nutrients, promotes the solubilization of hydrophobic organic compounds, coordinates cell-cell communication, facilitates gene exchange, and provides physical stability [ 58 ]. EPS also serves as a defensive barrier by preventing the entry of antibiotics into bacterial cells and increasing the resistance to antibacterial agents [ 46 ].…”

Section: Resultsmentioning

confidence: 99%

Biofabrication of ZnO nanoparticles using Acacia arabica leaf extract and their antibiofilm and antioxidant potential against foodborne pathogens

et al. 2022

View full text Add to dashboard Cite

Emergence of multidrug resistant pathogens is increasing globally at an alarming rate with a need to discover novel and effective methods to cope infections due to these pathogens. Green nanoparticles have gained attention to be used as efficient therapeutic agents because of their safety and reliability. In the present study, we prepared zinc oxide nanoparticles (ZnO NPs) from aqueous leaf extract of Acacia arabica. The nanoparticles produced were characterized through UV-Visible spectroscopy, scanning electron microscopy, and X-ray diffraction. In vitro antibacterial susceptibility testing against foodborne pathogens was done by agar well diffusion, growth kinetics and broth microdilution assays. Effect of ZnO NPs on biofilm formation (both qualitatively and quantitatively) and exopolysaccharide (EPS) production was also determined. Antioxidant potential of green synthesized nanoparticles was detected by DPPH radical scavenging assay. The cytotoxicity studies of nanoparticles were also performed against HeLa cell lines. The results revealed that diameter of zones of inhibition against foodborne pathogens was found to be 16–30 nm, whereas the values of MIC and MBC ranged between 31.25–62.5 μg/ml. Growth kinetics revealed nanoparticles bactericidal potential after 3 hours incubation at 2 × MIC for E. coli while for S. aureus and S. enterica reached after 2 hours of incubation at 2 × MIC, 4 × MIC, and 8 × MIC. 32.5–71.0% inhibition was observed for biofilm formation. Almost 50.6–65.1% (wet weight) and 44.6–57.8% (dry weight) of EPS production was decreased after treatment with sub-inhibitory concentrations of nanoparticles. Radical scavenging potential of nanoparticles increased in a dose dependent manner and value ranged from 19.25 to 73.15%. Whereas cytotoxicity studies revealed non-toxic nature of nanoparticles at the concentrations tested. The present study suggests that green synthesized ZnO NPs can substitute chemical drugs against antibiotic resistant foodborne pathogens.

show abstract

Section: Resultsmentioning

confidence: 99%

Biofabrication of ZnO nanoparticles using Acacia arabica leaf extract and their antibiofilm and antioxidant potential against foodborne pathogens

et al. 2022

View full text Add to dashboard Cite

show abstract

“…The zeta potential of several species of cyanobacteria, namely Tetraselmis suecica, Chlorococum sp., Chlorella sp. XJ-445 and Desmodesmus bijugatus XJ-231 has been reported to become less electronegative as cultures transition from the exponential to the stationary phase [38,39]. The zeta potential of the PCC7942 cells may be exhibiting similar behaviour.…”

Section: Discussionmentioning

confidence: 99%

Quantitative analysis of the effects of morphological changes on extracellular electron transfer rates in cyanobacteria

Okedi

Fisher

Yunus

2020

Biotechnol Biofuels

View full text Add to dashboard Cite

Background: Understanding the extracellular electron transport pathways in cyanobacteria is a major factor towards developing biophotovoltaics. Stressing cyanobacteria cells environmentally and then probing changes in physiology or metabolism following a significant change in electron transfer rates is a common approach for investigating the electron path from cell to electrode. However, such studies have not explored how the cells' concurrent morphological adaptations to the applied stresses affect electron transfer rates. In this paper, we establish a ratio to quantify this effect in mediated systems and apply it to Synechococcus elongatus sp. PCC7942 cells grown under different nutritional regimes. Results: The results provide evidence that wider and longer cells with larger surface areas have faster mediated electron transfer rates. For rod-shaped cells, increase in cell area as a result of cell elongation more than compensates for the associated decline in mass transfer coefficients, resulting in faster electron transfer. In addition, the results demonstrate that the extent to which morphological adaptations account for the changes in electron transfer rates changes over the bacterial growth cycle, such that investigations probing physiological and metabolic changes are meaningful only at certain time periods. Conclusion: A simple ratio for quantitatively evaluating the effects of cell morphology adaptations on electron transfer rates has been defined. Furthermore, the study points to engineering cell shape, either via environmental conditioning or genetic engineering, as a potential strategy for improving the performance of biophotovoltaic devices.

show abstract

“…Our results show that these three metrics (in vivo fluorescence, Chl a and cell abundance) yield consistently higher values of µ max than those obtained with biomass metrics (POC and PON), which reflects changes in resource allocation associated with unbalanced growth. Upon transfer to nutrient-rich medium in batch cultures, the population enters the exponential growth phase and cells allocate more resources into photosynthetic machinery 18 , 19 while their cell size decreases as population growth proceeds fast 20 , 21 , in both cases leading to estimates of µ max that exceed the actual rate of production of new biomass. Although the temperature dependence of growth rate is expected to be the same whatever metric is employed, the absolute value of growth rate is likely to differ depending on the metric used, which can affect some comparisons between studies.…”

Section: Discussionmentioning

confidence: 99%

Effect of temperature on the unimodal size scaling of phytoplankton growth

Fernández-González

Marañón

2021

Sci Rep

View full text Add to dashboard Cite

Contrary to predictions by the allometric theory, there is evidence that phytoplankton growth rates peak at intermediate cell sizes. However, it is still unknown if this pattern may result from the effect of experimental temperature. Here we test whether temperature affects the unimodal size scaling pattern of phytoplankton growth by (1) growing Synechococcus sp., Ostreococcus tauri, Micromonas commoda and Pavlova lutheri at 18 °C and 25 °C, and (2) using thermal response curves available in the literature to estimate the growth rate at 25 °C as well as the maximum growth rate at optimal temperature for 22 species assayed previously at 18 °C. We also assess the sensitivity of growth rate estimates to the metric employed for measuring standing stocks, by calculating growth rates based on in vivo fluorescence, chlorophyll a concentration, cell abundance and biomass (particulate organic carbon and nitrogen content). Our results show that the unimodal size scaling pattern of phytoplankton growth, with a peak at intermediate cell sizes, is observed at 18 °C, 25 °C and at the optimal temperature for growth, and that it prevails irrespective of the standing-stock metric used. The unimodal size scaling pattern of phytoplankton growth is supported by two independent field observations reported in the literature: (i) a positive relationship between cell size and metabolic rate in the picophytoplankton size range and (ii) the dominance of intermediate-size cells in nutrient-rich waters during blooms.

show abstract

The effect of growth phase on the surface properties of three oleaginous microalgae (Botryococcus sp. FACGB-762, Chlorella sp. XJ-445 and Desmodesmus bijugatus XJ-231)

Cited by 37 publications

References 30 publications

Biofabrication of ZnO nanoparticles using Acacia arabica leaf extract and their antibiofilm and antioxidant potential against foodborne pathogens

Biofabrication of ZnO nanoparticles using Acacia arabica leaf extract and their antibiofilm and antioxidant potential against foodborne pathogens

Quantitative analysis of the effects of morphological changes on extracellular electron transfer rates in cyanobacteria

Effect of temperature on the unimodal size scaling of phytoplankton growth

Contact Info

Product

Resources

About