The Effect of Green Tea on IL-6 and CRP level in Model of Polycystic Ovary Syndrome as an Inflammation State

Abstract

Background: Having low-grade chronic inflammation state symptoms such as elevated C-reactive protein and interleukin-6 play a crucial role in polycystic ovary syndrome (PCOS). Green tea has antiinflammatory properties. This research evaluates the effect of green tea on inflammatory indexes. Adult Wistar rats were exposed to subcutaneous administration of estradiol valerate for PCOS induction. PCOS rats were divided into control and experimental groups received intraperitoneal injection green tea extract daily. Animals were anesthesia with chloroform. Ovary and serum were taken to measure the inflammatory markes using ELISA kits and Histomorphometric studies. The data were analyzed using One-Way ANOVA with significance level P<0.05.Results: The results indicated the significant reduction in inflammatory markers and significant changes follicular layers thickness in the treatment group as compared with control.Disscoution: it can be concluded that having anti-inflammatory substances, green tea is effective in symptoms of this syndrome and metabolic syndrome.Keywords: Polycystic ovary syndrome, Wistar rat, green tea extract, inflammation, ELISA. BackgroundCytokines play a major role in response to the inflammatory stimuli and tissue damages. Interleukin (IL) -6 (20-30 kD aglycoprotein) is a pleiotropic cytokine, which is mainly produced by immune system and different types of adipose tissue, hepatocytes, and ovarian follicular granulosa [1]. Interleukin receptors are made of two sets, including GP-130 and il-6r. Interleukin-6 is on 7p21 chromosome. International Journal of Cellular & Molecular Biotechnology 2014 (2014 [1][2][3][4][5][6][7][8][9][10][11][12] Available online at www.ispacs.com/ijcmb is basically regulated by Beta Kappa core factor (NK-kb) and interleukin IL-6 causes stimulus, regulates synthesis of acute phase protein, activates axis of hypothalamus -pituitary and disorder in signal transduction of glucose through changing activity of serine -threonine kinases. Interleukin-6 plays a key role in the pathogenesis of chronic inflammation, insulin resistance, and CV diseases [2]. CRP is a member of pentraxinsfamily. As the most sensitive inflammatory indices, CRP plays an important role in immune response. In human, CRP gene lies on 1q21 to 1q23 chromosomes. CRP protein is produced by liver and it is almost the strongest inflammatory marker, as its increase is responsible for inflammation. Appearance of CRP is considered as a strong predicator for metabolic abnormality. This substance is produced in liver and intimal of vessels. Despite CRP's inflammatory role, using different mechanisms, such as producing nitric oxide (NO), increasing molecules adhesion, and changing absorption of low-density lipoprotein (LDL) by macrophages, it is able to damage vessels. The increase of this protein (as an independent predicator of CV risk) increases damages to CV system 2 -5 times. Its content is mainly regulated by some cytokines, especially 1L6 [2]. PCOS is a reproduction abnormality together with a metab...

The Impact of Green Tea Supplementation on Anthropometric Indices and Inflammatory Cytokines in Women with Polycystic Ovary Syndrome

“…Green tea and green tea catechins have been shown to influence plasma levels of inflammation markers in several diseases that related to inflammation (Stote et al, 2012;Uchiyama et al, 2013;Fukuzawa et al, 2014). Ghafurniyan et al (2014) showed that intraperitoneal injection of green tea extract in Wistar rats with PCOS, caused significant reduction in CRP and IL-6 levels after 14 days, but this finding is not consistent with our results; however, this was an animal study. Also, Zolfaghary et al (2013) indicated that consumption of green tea extract (three-capsule extract for 12 weeks) led to significant reduction in CRP levels in obese women, which does not match with our study, and it was probably a result of the longer intervention period compared with our study.…”

“…Ghafurniyan et al . () showed that intraperitoneal injection of green tea extract in Wistar rats with PCOS, caused significant reduction in CRP and IL‐6 levels after 14 days, but this finding is not consistent with our results; however, this was an animal study. Also, Zolfaghary et al .…”

A comprehensive insight into effects of green tea extract in polycystic ovary syndrome: a systematic review

“… Ghafurniya et al 2015 [ 23 ] 96 Wistar rats were divided into 4 groups: PCOS group, three experimental groups, which were given 50, 100 and 200 mg/Kg BW green tea extract 10 days, intraperitoneally. 50,100,200 mg/kg/day GTE 10 day -LH serum level, body weight and ovarian weight decreased -Insulin resistance and blood glucose decreased -Improved of ovarian function Ghafurniya et al 2014 [ 24 ] 40 Wistar rats were divided into 5 groups: PCOS rats received no injections (PCOS control), rats received saline (sham), PCOS rats that received of 50 mg/kg of green tea extract (GT-Treated-1), rats that were received intraperitoneal injection 100 mg/kg of green tea extract (GT-Treated-2), rats that were received intraperitoneal injection 200 mg/kg of green tea extract (GT-Treated-3). 50,100,200 mg/kg/day GTE 2 weeks –CRP and IL-6 serum levels reduced.…”

“…Insulin resistance and hyperglycemia are the most common features of patients with PCOS [ 29 ]. In this regard, Ghafurniyan et al [ 24 ] reported positive effects of statistically significant from a 200 mg/kg injection of GTE for 10 days on insulin level in PCOS model rats [ 24 ]. Similarly, a statistically significant decrease in insulin resistance was reported after consumption of GTE in obese and overweight PCOS women ( P < 0.05) [ 26 ].…”

“…In a study by Ghafurniya et al, a statistically significant decreasing effect of GTE injection on theca cell layer thickness and number of follicular cysts were seen in PCOS model rats [ 24 ]. In another animal study, Sadoughi et al found that GTE administration over 24 days led to a statistically significant decrease in serum levels of LH, β-estradiol, and testosterone in PCOS model rats, as well as improved ovarian function (Significant increase in the number of preantral, antral, prehavulatory and corpus luteum follicles and also caused a significant decrease in the number of cystic follicles), FSH, and progesterone levels, were also reported [ 21 ].…”