The Effect of Green Synthesized CuO Nanoparticles on Callogenesis and Regeneration of Oryza sativa L.

Abstract

In this study, we have investigated the effect of copper oxide nanoparticles (CuO-NPs) on callogenesis and regeneration of Oryza sativa L (Super Basmati, Basmati 2000, Basmati 370, and Basmati 385). In this regard, CuO-NPs have been bio-synthesized via Azadirachta indica leaf extract. Scanning electron microscope (SEM) analysis depicts average particle size of 40 ± 5 nm with highly homogenous and spherical morphology. X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) have been employed to confirm the phase purity of the synthesized NPs. It is found that CuO-NPs exhibit very promising results against callus induction. It is attributed to the fact that green synthesized CuO-NPs at optimum dosage possess very supportive effects on plant growth parameters. In contrast to callogenesis, differential regeneration pattern has been observed against all of the examined O. sativa L. indigenous verities. Overall observation concludes that CuO, being one of the essential plant nutrients, has greatly tailored the nutritive properties at nano-scale.

RETRACTED: CuO and CeO2 Nanostructures Green Synthesized Using Olive Leaf Extract Inhibits the Growth of Highly Virulent Multidrug Resistant Bacteria

“…Among metal oxide NS experimented so far, the performance of CuO-NS and CeO 2 -NS was found extremely effective in biological applications ( Anwaar et al, 2016 ). CeO 2 -NS exhibits variable oxidation states, stable crystal structure, greater surface energy, high surface to charge ratio, visible light activation, and greater stability making it as an ideal candidate for biomedical applications ( Charbgoo et al, 2017 ; Kamila and Venugopal, 2017 ; Chen and Stephen Inbaraj, 2018 ).…”

Retracted Article: Organometallic Ag nanostructures prepared using Hypericum perforatum extract are highly effective against multidrug-resistant bacteria

“…Usually, this alteration in the morphology of NS is very common when NS are prepared from plant extracts, as observed in previous investigations. 41,55 Some studies also conrmed that an irregular surface morphology of NS increased the overall surface area of the NS, which can enhance their reactivity for surface attachment from different reactive sites to the bacterial envelope during antibacterial action. 56,57 This occurs because of the squeezing of electrons on the nanoscale, which results in a quantum connement effect, which ultimately endows OM-Ag-NS with greater reactivity during antimicrobial action.…”

Nanomaterials in plant tissue culture: the disclosed and undisclosed

“…41 However, higher concentrations decreased the callus growth and enhanced antioxidant enzyme activity. Anwaar et al 70 reported that supplementation with CuO NPs (15-20 mg L À1 ) increased organogenesis in rice cultivars. In Daucus carota, cell proliferation and the number of somatic embryos decreased on MS medium containing Fe 3 O 4 .…”