The effect of green laser light irradiation on whole blood platelets

Grešner, Peter; Watała, Cezary; Šikurová, L

doi:10.1016/j.jphotobiol.2004.11.018

Cited by 12 publications

(8 citation statements)

References 20 publications

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“…However, one group previously reported that laser illumination of whole blood reduced platelet aggregation and activation in response to a thrombin agonist peptide . Other groups reported that low intensity laser illumination enhanced platelet secretion and production of reactive oxygen species …”

Section: Discussionmentioning

confidence: 98%

Low intensity laser therapy speeds wound healing in hemophilia by enhancing platelet procoagulant activity

Hoffman

Monroe

2012

Wound Repair Regeneration

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Our group has previously shown that cutaneous wound healing is delayed and histologically abnormal in a mouse model of hemophilia. Hemostasis is not only required to stop bleeding at the time of wounding, but also produces bioactive substances that promote appropriate inflammatory and proliferative responses during healing. Low intensity laser therapy (LILT) has been reported to enhance impaired wound healing in a variety of animal and human studies. The current studies were conducted to test the hypothesis that LILT can improve healing in a hemophilia B mouse model. Three daily treatments with 12 J/sq cm of 650 nm laser illumination reduced the time to closure of a 3-mm cutaneous punch biopsy wound in the hemophilic mice. All wounds were closed at 13 days in the sham-treated hemophilic mice, compared with 10 days in the LILT-treated hemophilic mice, and 9 days in wild-type mice. While LILT can speed healing by enhancing proliferation of cutaneous cells, we found that an additional mechanism likely contributes to the efficacy of LILT in the hemophilic mice. LILT enhanced the mechanical rigidity and platelet activity of clots formed from human platelet-rich plasma. Illumination of isolated platelets increased the mitochondrial membrane potential and enhanced binding of coagulation factors to the surface of activated platelets. Thus, while LILT can directly promote proliferative responses during healing, it also appears to enhance hemostasis in an animal model with impaired coagulation. These data suggest that trials of LILT as an adjunct to the usual hemostatic therapies in hemophilia are warranted.

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Section: Discussionmentioning

confidence: 98%

Low intensity laser therapy speeds wound healing in hemophilia by enhancing platelet procoagulant activity

Hoffman

Monroe

2012

Wound Repair Regeneration

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“…It would be reasonable to expect that the combination of therapies in group PRP/LLLT might be superior to the use of either therapy alone because laser light irradiation has been shown to activate platelets 22 . However, NC in group PRP/LLLT did not differ statistically from that of groups PRP or LLLT at 30 days post‐surgery.…”

Section: Discussionmentioning

confidence: 98%

“…In vitro studies have demonstrated that laser irradiation also induces platelet degranulation and the release of substances stored in the specific granules 21,22 . Therefore, it is possible that the combined use of PRP and LLLT could increase the concentration of GFs in the wound healing site.…”

mentioning

confidence: 99%

Platelet‐Rich Plasma, Low‐Level Laser Therapy, or Their Combination Promotes Periodontal Regeneration in Fenestration Defects: A Preliminary In Vivo Study

Nagata

Campos

Messora

et al. 2014

Journal of Periodontology

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“…Those studies have been performed using red to infra-red LLIs because of their penetration property into relatively deeper sites [ 7 ]. In cell culture systems using other wavelength LLIs, we can find reports showing that 532 nm LLI promoted proliferation of B-14 (Chinese hamster ovarian cell line) cells without inducing cell death [ 8 ], it influenced blood platelets to trigger signal transduction, leading to platelet activation [ 9 ], and it significantly increased cell survival of human adipose tissue-derived stem cells following mitochondria activation [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

532 nm Low-Power Laser Irradiation Facilitates the Migration of GABAergic Neural Stem/Progenitor Cells in Mouse Neocortex

et al. 2015

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Background and ObjectiveAccumulating evidence has shown that low-power laser irradiation (LLI) affects cell proliferation and survival, but little is known about LLI effects on neural stem/progenitor cells (NSPCs). Here we investigate whether transcranial 532 nm LLI affects NSPCs in adult murine neocortex and in neurospheres from embryonic mice.Study Design/Materials and MethodsWe applied 532 nm LLI (Nd:YVO4, CW, 60 mW) on neocortical surface via cranium in adult mice and on cultured cells from embryonic mouse brains in vitro to investigate the proliferation and migration of NSPCs and Akt expression using immunohistochemical assays and Western blotting techniques.ResultsIn vivo experiments demonstrated that 532 nm LLI significantly facilitated the migration of GABAergic NSPCs that were induced to proliferate in layer 1 by mild ischemia. In vitro experiments using GABAergic NSPCs derived from embryonic day 14 ganglionic eminence demonstrated that 532 nm LLI for 60 min promoted the migration of GAD67-immunopositive NSPCs with a significant increase of Akt expression. Meanwhile, the LLI induced proliferation, but not migration, of NSPCs that give rise to excitatory neurons.ConclusionIt is concluded that 532 nm LLI promoted the migration of GABAergic NSPCs into deeper layers of the neocortex in vivo by elevating Akt expression.

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The effect of green laser light irradiation on whole blood platelets

Cited by 12 publications

References 20 publications

Low intensity laser therapy speeds wound healing in hemophilia by enhancing platelet procoagulant activity

Low intensity laser therapy speeds wound healing in hemophilia by enhancing platelet procoagulant activity

Platelet‐Rich Plasma, Low‐Level Laser Therapy, or Their Combination Promotes Periodontal Regeneration in Fenestration Defects: A Preliminary In Vivo Study

532 nm Low-Power Laser Irradiation Facilitates the Migration of GABAergic Neural Stem/Progenitor Cells in Mouse Neocortex

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