The effect of grafting method on the colloidal stability and in vitro cytotoxicity of carboxymethyl dextran coated magnetic nanoparticles

Creixell, Mar; Herrera, Adriana; Latorre-Esteves, Magda; Ayala, Vanessa; Torres‐Lugo, Madeline; Rinaldi, Carlos

doi:10.1039/c0jm01504k

Cited by 56 publications

(60 citation statements)

References 37 publications

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“…25 Miles et al described that phosphate salts are responsible for dextran molecules desorption from the nanoparticles surface when they are not covalently bound. 44 Hence, D coated nanocrystals were incubated in PBS buffer at pH 7.4 for 24…”

Section: Characterization Of Surface Modified Mncsmentioning

confidence: 99%

“…23 If dextran is attached to the nanoparticles surface by weak interactions as physical absorption, less control of coupled functional groups and, more important the coating loss in particular conditions (i.e. the physiological medium), could occur 25,26 with consequent nanoparticles aggregation and possible cytotoxicity. 27 So a strong binding between the polymer and the nanoparticles surface is preferred.…”

mentioning

confidence: 99%

“…13,23 Among them, dextran (a polysaccharide composed by -D-glucopyranosyl units) is preferred due to its extremely low toxicity by intravenous route, enhancement of blood circulation time and the presence of chemical groups for further functionalizations. 24,25 Some problems can be associated with the nanoparticles coating strategy, as an inhomogeneous surface coverage and a low bind strength. 23 If dextran is attached to the nanoparticles surface by weak interactions as physical absorption, less control of coupled functional groups and, more important the coating loss in particular conditions (i.e.…”

mentioning

confidence: 99%

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Large scale production of biocompatible magnetite nanocrystals with high saturation magnetization values through green aqueous synthesis

Marciello

Connord

Veintemillas-Verdaguer

et al. 2013

J. Mater. Chem. B

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In this work, a straightforward aqueous synthesis for mass production (up to 20 g) of uniform and crystalline magnetite nanoparticles with a core sizes between 20 and 30 nm, which are the optimum nanoparticle core sizes for hyperthermia application, is proposed. Magnetic and heating properties have been analyzed showing very high saturation magnetization and magnetic heating values. To stabilize the naked magnetite nanocrystals at physiological pH and increase their circulation time in blood, they have been covalently coated with carboxy-methyl-dextran, a biocompatible polymer.The influence of this superficial modification on magnetic and heating properties has been studied showing that these biocompatible magnetic nanocrystals maintain high magnetization saturation values, good colloidal stability and hyperthermia properties in the presence of the polymeric external layer. These particles, properly functionalized, could be used to selectively kill cancer cells under a moderate alternating magnetic field (44 mT and 70 kHz).

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Section: Characterization Of Surface Modified Mncsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Large scale production of biocompatible magnetite nanocrystals with high saturation magnetization values through green aqueous synthesis

Marciello

Connord

Veintemillas-Verdaguer

et al. 2013

J. Mater. Chem. B

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“…Note that the particles used in this study are similar to those reported in our recent work, in which additional chemical, physical, and magnetic characterization is provided. 24 cytocompatibility Cytocompatibilty analyses were first performed to rule out the effect of nanoparticle toxicity during MFH. Experiments were performed during cell seeding for a period of 1 week using two different cell culture models (Caco-2 and MCF-7).…”

Section: Particle Characterizationmentioning

confidence: 99%

Enhanced reduction in cell viability by hyperthermia induced by magnetic nanoparticles

Rodríguez-Luccioni¹,

Latorre-Esteves²,

Méndez-Vega³

et al. 2011

IJN

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Colloidal suspensions of iron oxide magnetic nanoparticles are known to dissipate energy when exposed to an oscillating magnetic field. Such energy dissipation can be employed to locally raise temperature inside a tumor between 41°C and 45°C (hyperthermia) to promote cell death, a treatment known as magnetic fluid hyperthermia (MFH). This work seeks to quantify differences between MFH and hot-water hyperthermia (HWH) in terms of reduction in cell viability using two cancer cell culture models, Caco-2 (human epithelial colorectal adenocarcinoma) and MCF-7 (human breast cancer). Magnetite nanoparticles were synthesized via the co-precipitation method and functionalized with adsorbed carboxymethyl dextran. Cytotoxicity studies indicated that in the absence of an oscillating magnetic field, cell viability was not affected at concentrations of up to 0.6 mg iron oxide/mL. MFH resulted in a significant decrease in cell viability when exposed to a magnetic field for 120 minutes and allowed to rest for 48 hours, compared with similar field applications, but with shorter resting time. The results presented here suggest that MFH most likely induces apoptosis in both cell types. When compared with HWH, MFH produced a significant reduction in cell viability, and these effects appear to be cell-type related.

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“…44,45 The methods for obtaining these nanoparticles have been published by our group, along with detailed characterization of their colloidal properties. [44][45][46] We refer to CMDx-coated IO obtained by coprecipitation as Cop-IO. The primary particle diameter determined by transmission electron microscopy (JEOL 1200 EX; Jeol Ltd, Tokyo, Japan) was 12±2 nm, and the hydrodynamic diameter, determined by dynamic light scattering (BI-90Plus; Brookhaven Instruments, Holtsville, NY, USA), was 77±5 nm.…”

mentioning

confidence: 99%

Tissue-specific direct microtransfer of nanomaterials into Drosophila embryos as a versatile in vivo test bed for nanomaterial toxicity assessment

Vega-Alvarez¹,

Herrera²,

Rinaldi³

et al. 2014

IJN

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Nanomaterials are the subject of intense research, focused on their synthesis, modification, and biomedical applications. Increased nanomaterial production and their wide range of applications imply a higher risk of human and environmental exposure. Unfortunately, neither environmental effects nor toxicity of nanomaterials to organisms are fully understood. Cost-effective, rapid toxicity assays requiring minimal amounts of materials are needed to establish both their biomedical potential and environmental safety standards. Drosophila exemplifies an efficient and cost-effective model organism with a vast repertoire of in vivo tools and techniques, all with high-throughput scalability and screening feasibility throughout its life cycle. Here we report tissue specific nanomaterial assessment through direct microtransfer into target tissues. We tested several nanomaterials with potential biomedical applications such as single-wall carbon nanotubes, multiwall carbon nanotubes, silver, gold, titanium dioxide, and iron oxide nanoparticles. Assessment of nanomaterial toxicity was conducted by evaluating progression through developmental morphological milestones in Drosophila . This cost-effective assessment method is amenable to high-throughput screening.

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The effect of grafting method on the colloidal stability and in vitro cytotoxicity of carboxymethyl dextran coated magnetic nanoparticles

Cited by 56 publications

References 37 publications

Large scale production of biocompatible magnetite nanocrystals with high saturation magnetization values through green aqueous synthesis

Large scale production of biocompatible magnetite nanocrystals with high saturation magnetization values through green aqueous synthesis

Enhanced reduction in cell viability by hyperthermia induced by magnetic nanoparticles

Tissue-specific direct microtransfer of nanomaterials into Drosophila embryos as a versatile in vivo test bed for nanomaterial toxicity assessment

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