Enhanced reduction in cell viability by hyperthermia induced by magnetic nanoparticles

Abstract

Colloidal suspensions of iron oxide magnetic nanoparticles are known to dissipate energy when exposed to an oscillating magnetic field. Such energy dissipation can be employed to locally raise temperature inside a tumor between 41°C and 45°C (hyperthermia) to promote cell death, a treatment known as magnetic fluid hyperthermia (MFH). This work seeks to quantify differences between MFH and hot-water hyperthermia (HWH) in terms of reduction in cell viability using two cancer cell culture models, Caco-2 (human epithelial colorectal adenocarcinoma) and MCF-7 (human breast cancer). Magnetite nanoparticles were synthesized via the co-precipitation method and functionalized with adsorbed carboxymethyl dextran. Cytotoxicity studies indicated that in the absence of an oscillating magnetic field, cell viability was not affected at concentrations of up to 0.6 mg iron oxide/mL. MFH resulted in a significant decrease in cell viability when exposed to a magnetic field for 120 minutes and allowed to rest for 48 hours, compared with similar field applications, but with shorter resting time. The results presented here suggest that MFH most likely induces apoptosis in both cell types. When compared with HWH, MFH produced a significant reduction in cell viability, and these effects appear to be cell-type related.

Nanoscale Thermal Phenomena in the Vicinity of Magnetic Nanoparticles in Alternating Magnetic Fields

“…Rodriguez-Luccioni et al (2011) showed in vitro that heating with MNPs resulted in greater decrease in cell viability over hot water hyperthermia under similar time-temperature conditions. [35] Lee et al (2011) showed that much lower doses of cisplatin are effective in combination with MFH, enhancing the anticancer activity of cisplatin more than hot water bath hyperthermia. [14a] Alvarez-Berrios et al (2013) demonstrated that fluidization of the cell membrane occurs due to heating with MNPs, which enhances drug uptake [15] and that MFH has synergy with cisplatin even in resistant cell lines.…”

Impact of magnetic field parameters and iron oxide nanoparticle properties on heat generation for use in magnetic hyperthermia

“…MFH has also been shown to kill cells faster as compared to traditional hyperthermia methods, which can play an essential role in reducing the therapy administration time for cancer treatment [4]. MFH can reduce side effects in patients while amplifying treatment of cancer using superparamagnetic NPs, which can be specifically targeted using antibodies or peptide sequences [5,6] and directed to cancerous tissue through the enhanced permeation and retention (EPR) effect [7].…”

Role of CTGF in Sensitivity to Hyperthermia in Ovarian and Uterine Cancers

“…Different types of energy has been used, including microwaves and radio waves (Gazelle et al, 2000; Seki et al, 1999), magnetic heating (Lee et al, 2011; Rodriguez-Luccioni et al, 2011), and ultrasound (Jolesz and Hynynen, 2002). Regional hyperthermia (e.g., hyperthermic intraperitoneal chemotherapy [HIPEC] heated to 42°C), theoretically enables direct contact between tumor cells and the chemotherapeutic agent to control residual microscopic disease (Jinny Ha, 2012).…”