The effect of glycerol as a cryoprotective agent in the cryopreservation of adipose tissue

Zhang, Pei-Qi; Tan, Poh-Ching; Gao, Yiming; Zhang, Xiaojie; Xie, Yun; Zheng, Duo; Zhou, Shuang-Bai; Li, Qingfeng

doi:10.1186/s13287-022-02817-z

Cited by 18 publications

(13 citation statements)

References 36 publications

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“…It is unfortunate that there is now no best strategy for the long-term preservation of adipose tissues, and research that attempt to find such a way are few and not particularly productive. 28 That's why in this study we aimed to compare the survival rates of freshly liposuctioned fat and cryopreserved fat using 3 different freezing methods including direct freezing, freezing in the adi-frosty containing 100% isopropanol and freezing solution with 90% FBS (v/v) and 10% DMSO (v/v). Our ideal cryopreservation strategy retained much more live adipocytes and greater cellular function in adipose aspirates than a straightforward cryopreservation procedure.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Adipocyte Viability After Short-Term Cryopreservation of Adipose Aspirates Through 3 Different Techniques

Koçak

Ünsal

Canikyan

et al. 2023

Aesthetic Surgery Journal Open Forum

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Background Effective cryopreservation allows for the long-term storage of living cells or tissues with the possibility of later clinical applications. Unfortunately, no successful investigations on the long-term preservation of adipose aspirates for prospective autologous fat grafting have been conducted. Objectives In this study, we aimed to compare three different freezing methods to preserve adipose aspirates obtained from conventional lipoplasty to determine theoptimal cryopreservation technique. Methods To determine the optimal cryopreservation technique hematoxylin and eosin (H&E) staining, MTT assay and Annexin Assay were performed on each of the three groups plus a fourth control group. different. Group 1 served as the control and fat tissue was analyzed immediately after adipose harvesting with no cryopreservation. For experimental group 2, 15 mL of adipose aspirates were directly frozen at -80oC for up to two weeks. For experimental group 3, 15 mL of adipose aspirates were frozen inside the adi-frosty containing 100% isopropanol and stored at -80°C for up to two weeks. For experimental group 4, 15 mL of adipose aspirates were frozen with freezing solution containing 90% of Fetal Bovine Serum (FBS) (v/v) and 10% of Dimethyl sulfoxide (DMSO) (v/v). Results The results demonstrated that the experimental group 3 had significantly more live adipocytes and greater cellular function of adipose aspirates than the experimental groups 2 and 4. Conclusions Cryopreservation with adi-frosty containing 100% isopropanol appears to be the best means of cryopreservation of fat.

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Section: Discussionmentioning

confidence: 99%

Comparison of Adipocyte Viability After Short-Term Cryopreservation of Adipose Aspirates Through 3 Different Techniques

Koçak

Ünsal

Canikyan

et al. 2023

Aesthetic Surgery Journal Open Forum

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“…However, the better performance of TFE diluter might be due to the use of glycerol to a slightly higher percentage [14,28]. Glycerol is a widely used cryoprotectant in freeze-thawing that reduce the formation of intracellular ice crystal by entering the cells and binding with water content [15]. Besides this intracellular cryoprotectant also minimizes the difference in osmotic pressure.…”

Section: Discussionmentioning

confidence: 99%

Comparison between Manual and Automated Cryopreservation Techniques and Effects of Different Semen Extenders on Post-Thaw Semen Quality of Buffalo

Kabir¹,

Hossain²,

Deb³

et al. 2022

Preprint

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Cryopreservation has been used extensively for cattle in Bangladesh, albeit no study was conducted on the cryopreservation techniques of buffalo. This study compares two freezing methods and the effects of diluters on the semen quality of buffalo. In the first freezing protocol, semen was frozen in two-step: from 37 °C to 5 °C for 30 minutes in a BLRI-developed equilibration chamber and from 5 oC to -120 oC in a Styrofoam box using liquid nitrogen vapor from different distances (0.5, 1.5, 1.6, 2 and 3 inches). At the same time semen was frozen in a programmable freezer in three steps. The semen samples were then evaluated for motility and morphological quality by CASA. In another experiment, the efficacy of one locally developed diluter-Tris-fructose-egg yolk-based (TFE) and three commercial diluters (Andromed, Triladyl and Steridyl) were evaluated. The highest number of motile sperms (62.67±1.12; P< 0.01) and progressive motility (38.97±1.10; P < 0.001) was observed at 1.6 inches above liquid nitrogen. There was no significant difference in overall motility, progressive and slow motility between semen cryopreserved in the cheap nitrogen vapor technique (57.49±5.67, 38.70± 4.04 and 3.83± 0.63, respectively) and expensive automated technique (65.94±4.65, 45.54 ± 3.64 and 2.43± 0.36, respectively). The highest recovery rate and conception rate were observed in semen diluted with TFE (82.4% and 80%, respectively). Hence, the cryopreservation technique using nitrogen vapor and TFE diluent is cost-effective and suitable for freezing buffalo semen that would produce superior semen for artificial insemination.

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“…• Cytotoxicity and necessity to remove after thawing Glycerol [ 104,171,172] • Forms hydrogen bonds with water molecules, and competes with those between water molecules…”

Section: • Increases Viscosity Of Cryogenic Mediummentioning

confidence: 99%

Cryopreservation in the Era of Cell Therapy: Revisiting Fundamental Concepts to Enable Future Technologies

Bai

Xue

Yang

et al. 2023

Adv Funct Materials

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Cryopreservation strives to maximize the viability and biofunctionality of cells and tissues by cooling them to a subzero temperature to facilitate storage and delivery. This technology has enabled clinics and labs to preserve rare and crucial samples and is poised to become more important with rising interest in cell therapy. Here, the biological impact of cooling rates on different cellular components is first described, paying special emphasis on the differences between slow cooling and vitrification with a heat transfer perspective based on the Biot number. This is followed by an overview of various classes of chemical‐based cryoprotective agents including small molecules, antifreeze proteins, hydrogels, and cryoprotective nanomaterials. Most importantly, fundamental concepts of cryopreservation including Mazur's “two‐factor hypothesis” are revisited, gaps in them are highlighted, and experiments to validate reported claims to deepen mechanistic understanding of cryoprotection are proposed. A matric is also introduced to assess the suitability of biomaterials for use in cell therapy to support manufacturers in making strategic choices for storing clinical samples. It is believed that this review would inspire readers to scrutinize fundamental concepts in cryopreservation to facilitate the development of new cryoprotective materials and technologies to support the emerging cell manufacturing and therapy industry.

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The effect of glycerol as a cryoprotective agent in the cryopreservation of adipose tissue

Cited by 18 publications

References 36 publications

Comparison of Adipocyte Viability After Short-Term Cryopreservation of Adipose Aspirates Through 3 Different Techniques

Comparison of Adipocyte Viability After Short-Term Cryopreservation of Adipose Aspirates Through 3 Different Techniques

Comparison between Manual and Automated Cryopreservation Techniques and Effects of Different Semen Extenders on Post-Thaw Semen Quality of Buffalo

Cryopreservation in the Era of Cell Therapy: Revisiting Fundamental Concepts to Enable Future Technologies

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