The effect of gamma radiation on seed germination and seedling growth of Lathyrus chrysanthus Boiss. under in vitro conditions

Beyaz, Ramazan; Kahramanogulları, Cansu Telci; Yildiz, Çiğdem; Darçın, Emine Selcen; Yıldız, Mustafa

doi:10.1016/j.jenvrad.2016.05.006

Cited by 62 publications

(34 citation statements)

References 25 publications

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“…Telci et al [2011] reported that sodium hypochlorite (NaOCl) solutions used as a chemical method could also be successfully used as a dormancy-breaking agent in seeds of L. chrysanthus Boiss. Beyaz et al [2016] investigated the effects of radiation at different doses (0-control, 50, 100, 150, 200, and 250 Gy) of radioactive cobalt (60Co) gamma rays on seed germination and seedling growth of Lathyrus chrysanthus under in vitro conditions. Also, Yildiz [2012] demonstrated that seedborne contamination increased gradually by decreasing concentrations and application periods of NaOCl below 3.75% (75% commercial bleach containing 5% NaOCl) and 15 min in Lathyrus chrysanthus seeds.…”

Section: Discussionmentioning

confidence: 99%

“…Treated seeds with 125 mT MF and untreated seeds were surface-sterilized using 75% commercial bleach (containing 5% sodium hypochlorite) at 35 8C for 15 min with continuous stirring and were then rinsed three times with sterile distilled water (sdH 2 0) at the same temperature as reported in previous studies [Yildiz and Er, 2002;Telci et al, 2011;Beyaz et al, 2016]. Sterilized seeds were shaken in sdH 2 0 for 6 h to increase the permeability of seed coat and then were germinated on a basal medium of Murashige and Skoog's (MS) mineral salts and vitamins [Murashige and Skoog, 1962], 3% sucrose, and 0.7% agar in Magenta vessels (150 Â 150 mm).…”

Section: Surface Sterilization and In Vitro Seed Germinationmentioning

confidence: 99%

“…Lathyrus chrysanthus is an ornamental plant with big, colored, and fragrant and non-fragrant flowers [Davis, 1970;Telci et al, 2011]. Lathyrus chrysanthus seeds are not cultivated extensively because they have low germination frequency [Beyaz et al, 2016]. Telci et al [2011] have reported that dormancy in Lathyrus chrysanthus Boiss.…”

Section: Introductionmentioning

confidence: 99%

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Effect of magnetic field on in vitro seedling growth and shoot regeneration from cotyledon node explants of Lathyrus chrysanthus boiss

Bahadir

Beyaz

Yıldız

2018

Bioelectromagnetics

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The stimulatory effects on germination of seeds and growth of plants of static magnetic field (MF) pre-treatments depending on MF intensity, exposure time periods, signal form, flux density, and source frequencies on plants are reported. Seed germination frequency is low due to dormancy in Lathyrus chrysanthus Boiss. from Fabaceae family, consisting of 187 taxa. Tissue culture protocol for this plant has already been optimized. This plant is also used as a model for developing alternative methods to overcome dormancy. This study was conducted to determine the effects of MF on in vitro seed germination, seedling growth, and shoot regeneration capacity of cotyledon node explants in Lathyrus chrysanthus Boiss. to obtain healthy seedlings in large quantities. The seeds of an ecotype (Diyarbakir) were subjected to 125 mT MF strength for different exposure time periods (0-untreated, 24, 48, and 72 h). Sterilized seeds were germinated on growth basal medium in Magenta vessels. Seed germination and seedling growth percentages were recorded after 7 and 14 days of culture initiation, whereas seedling and root lengths were noted 28 days after culture initiation. At the end of the culture, shoot regeneration percentage, shoot number per explant, highest shoot height per explant, and total shoot number per petri dish were recorded. According to the results, it could be concluded that MF treatment could clearly be used to improve germination by breaking dormancy not only in Lathyrus chrysanthus Boiss. but also other plant species.

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Section: Discussionmentioning

confidence: 99%

Section: Surface Sterilization and In Vitro Seed Germinationmentioning

confidence: 99%

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Effect of magnetic field on in vitro seedling growth and shoot regeneration from cotyledon node explants of Lathyrus chrysanthus boiss

Bahadir

Beyaz

Yıldız

2018

Bioelectromagnetics

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“…In the study conducted by Beyaz et al [72], the efects of gamma radiation on overcoming dormancy in seeds of L. chrysanthus Boiss. under in vitro conditions were examined.…”

Section: Gamma Radiationmentioning

confidence: 99%

Seed Dormancy

Yıldız¹,

Beyaz²,

Gürsoy³

et al. 2017

Advances in Seed Biology

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Dormancy is when there is a lack of germination in seeds or tubers even though the required conditions (temperature, humidity, oxygen, and light) are provided. Dormancy is based on hard seed coat impermeability or the lack of supply and activity of enzymes (internal dormancy) necessary for germination. Dormancy is an important factor limiting production in many ield crops. Several physical and chemical pretreatments are applied to the organic material (seeds/tubers) to overcome dormancy. Physical and physiological dormancy can be found together in some plants, and this makes it diicult to provide high-frequency, healthy seedling growth, since the formation of healthy seedlings from the organic material (seeds/tubers) sown is a prerequisite for plant production. This chapter will focus on the description of four diferent methods we have not seen reported elsewhere for overcoming dormancy.

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“…Several research on the stimulative effects when using gamma rays as induced mutagens were reported. Recently, low doses of gamma-rays of seed treatment resulted significant effects on germination, shoot, and root systems of wheat [13], physic nut [14], rice [15], maize [16], pigeon pea [17], chick pea [18], lathyrus [19], castor bean [20].However, the impact of gamma rays on soybean cv. Dering 1 is still unknown.…”

Section: Introductionmentioning

confidence: 99%

Mutagenic effects of gamma rays on soybean (Glycine max L.) germination and seedlings

Kusmiyati

Sutarno

Sas

et al. 2018

IOP Conf. Ser.: Earth Environ. Sci.

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Abstract. Narrow genetic diversity is a main problem restricting the progress of soybean breeding. One way to improve genetic diversity of plant is through mutation. The purpose of this study was to investigate effect of different dose of gamma rays as induced mutagen on physiological, morphological, and anatomical markers during seed germination and seedling growth of soybean. Seeds of soybean cultivars Dering-1 were irradiated with 11 doses of gamma rays (0, 5, 10, 20, 40, 80, 160, 320, 640, 1280, and 2560 Gy [Gray]. The research design was arranged in a completely randomized block design in three replicates. Results showed that soybean seed exposed at high doses (640, 1280, and 2560 Gy) did not survive more than 20 days, the doses were then removed from anatomical evaluation. Higher doses of gamma rays siginificantly reduced germination percentage at the first count and final count, coefficient of germination velocity, germination rate index, germination index, seedling height and seedling root length, and significantly increased mean germination time, first day of germination, last day of germination, and time spread of germination. However, the effects of gamma rays were varies for density, width, and length of stomata. The LD 50 obtained based on survival percentage was 314.78 Gy. It can be concluded that very low and low doses of gamma rays (5-320 Gy) might be used to study the improvement of soybean diversity.

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The effect of gamma radiation on seed germination and seedling growth of Lathyrus chrysanthus Boiss. under in vitro conditions

Cited by 62 publications

References 25 publications

Effect of magnetic field on in vitro seedling growth and shoot regeneration from cotyledon node explants of Lathyrus chrysanthus boiss

Effect of magnetic field on in vitro seedling growth and shoot regeneration from cotyledon node explants of Lathyrus chrysanthus boiss

Seed Dormancy

Mutagenic effects of gamma rays on soybean (Glycine max L.) germination and seedlings

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