The Effect of Fluorine on Mouth Bacteria

Bibby, Basil G.; Kesteren, M. Van

doi:10.1177/00220345400190040601

Cited by 132 publications

(50 citation statements)

References 11 publications

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“…In studies of in vitro systems, however, several investigators have demonstrated that 1 -1 0 ppm fluoride can reduce acid production by salivary micro-organisms [Bibby and V an Kesteren, 1940;Clapper, 1947;Jenkins, 1959]. Briner and F rancis [1962] have shown that acid production by bacteria could also be inhibited by fluoride released from enamel treated with sodium or stannous fluoride, or from mottled enamel.…”

Section: Discussionmentioning

confidence: 99%

The Inhibition of Enolase by Fluoride in vitro

Cimasoni¹

1972

Caries Res

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The activity of a commercial preparation of muscle enolase was determined photometrically. The sensitivity of the enzyme to fluoride and the kinetics of the inhibition was studied in the absence and in the presence of 5 mM phosphate. The effects of fluoride, magnesium, phosphate and fluorophosphate were investigated by introducing these ions alone or in combination in the assay medium during automatic recording. The inhibition of enolase in vitro started at 0.5 ppm fluoride in the presence of phosphate, and at 10 ppm in the absence of phosphate ions. The Lineweaver-Burk (1/V-1/[S]) and the Dixon (1/V-[F^-])plots show that, in the presence of phosphate, the inhibition of enolase by fluoride is of the competitive type. In the absence of phosphate, the kinetics indicate a mechanism of inhibition identical to that found for enzymes without metal ion cofactors. When introduced in the assay medium during recording, fluoride produced a change of slope which was progressive and required 10-15 sec to be complete. The inhibition of enolase in the absence of phosphate, the results of the kinetic studies and those on the rapidity of the inhibitory action confirm that the inhibition of the enolase reaction is probably not caused by a purely inorganic complex.

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Section: Discussionmentioning

confidence: 99%

The Inhibition of Enolase by Fluoride in vitro

Cimasoni¹

1972

Caries Res

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“…This hy pothesis had been suggested by two groups of observations: (1) according to some authors [Armstrong, 1938;Bibby and V an Kestern, 1940;Po lice, 1960;Benagiano, Moschella and Sacca, 1962, in contrast with McClure, 1956 andT empestini, 1966] sound enamel from sound teeth contains more fluoride than sound enamel from carious teeth; (2) accord ing to Backer D irks [1963,1967], the effect of fluoridation in reducing dental caries is not the same for different surfaces of the tooth; in the Tiel-Culemborg experiment the caries protective action of fluoride, ex pressed as percentage reduction in caries, was found by Backer D irks to be greater on the approximal, buccal and lingual surfaces than on the oc clusal surfaces, and greater on the incisors than on the posterior teeth. In an earlier investigation Marci and Scavizzi [1967] examined, in a clini cal caries study, the teeth of residents of a low fluoride area (Rieti).…”

Section: Introductionmentioning

confidence: 85%

The Relationship between Fluoride Concentration and the Caries Frequency of Different Tooth Surfaces in a High Fluoride Area

Candeli¹,

Scavizzi²,

Marci³

1970

Caries Res

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A clinical investigation on caries attack of different dental surfaces was carried out in a high fluoride area (Campagnano) in 144 adults. At the same time the fluoride concentration of 269 samples of different dental surfaces of 65 caries-free teeth, belonging to adults from the same area, was determined. Statistical analysis of clinical and chemical data, and a comparison between these data and earlier data collected in a low fluoride area (Rieti) were carried out. Highly significant differences, both in caries susceptibility and in fluoride content, were found in Campagnano between the occlusal and the other four surfaces (the occlusal surfaces were the most affected by caries and the lowest in fluoride). In Rieti similar findings were observed in the clinical data but not in chemical data. All surfaces in Campagnano showed a significantly higher fluoride content than the surfaces in Rieti and a significantly lower percentage in caries. However, the increase in fluoride content varied for the different surfaces. Caries reduction was also not the same for different surfaces.

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“…Fluoride, a common additive in toothpaste, inhibits the glycolytic enzyme enolase, which leads to a diminished production of ATP and the acid end products of metabolism (4,16,28). Fluoride also inhibits the proton-translocating ATPases, which, coupled with a reduction in the intracellular concentration of ATP, affects the cell's ability to maintain the intracellular pH (20,29).…”

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confidence: 99%

Acid Tolerance of Biofilm Cells of Streptococcus mutans

Welin-Neilands

Svensäter

2007

Appl Environ Microbiol

115

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Streptococcus mutans, a member of the dental plaque community, has been shown to be involved in the carious process. Cells of S. mutans induce an acid tolerance response (ATR) when exposed to sublethal pH values that enhances their survival at a lower pH. Mature biofilm cells are more resistant to acid stress than planktonic cells. We were interested in studying the acid tolerance and ATR-inducing ability of newly adhered biofilm cells of S. mutans. All experiments were carried out using flow-cell systems, with acid tolerance tested by exposing 3-h biofilm cells to pH 3.0 for 2 h and counting the number of survivors by plating on blood agar. Acid adaptability experiments were conducted by exposing biofilm cells to pH 5.5 for 3 h and then lowering the pH to 3.5 for 30 min. The viability of the cells was assessed by staining the cells with LIVE/DEAD BacLight viability stain. Three-hour biofilm cells of three different strains of S. mutans were between 820-and 70,000-fold more acid tolerant than corresponding planktonic cells. These strains also induced an ATR that enhanced the viability at pH 3.5. The presence of fluoride (0.5 M) inhibited the induction of an ATR, with 77% fewer viable cells at pH 3.5 as a consequence. Our data suggest that adhesion to a surface is an important step in the development of acid tolerance in biofilm cells and that different strains of S. mutans possess different degrees of acid tolerance and ability to induce an ATR.

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The Effect of Fluorine on Mouth Bacteria

Cited by 132 publications

References 11 publications

The Inhibition of Enolase by Fluoride in vitro

The Inhibition of Enolase by Fluoride in vitro

The Relationship between Fluoride Concentration and the Caries Frequency of Different Tooth Surfaces in a High Fluoride Area

Acid Tolerance of Biofilm Cells of Streptococcus mutans

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