The Effect of Flunixin Meglumine Injected Before Embryo Transfer on Pregnancy Rates in Heifers

Abstract

[1] Bu çalışma, 4. Reprodüksiyon ve Suni Tohumlama Kongresi, 25-28 Ekim, 2007, Antalya'da sözlü bildiri olarak sunulmuştur * Bahri Dağdaş Uluslararası Tarımsal Araştırma Enstitüsü, Konya -TÜRKİYE Makale Kodu (Article Code): KVFD-2009-502 ÖzetBu çalışma ile, östrüsün 7. gününde (östrüs 0) embriyo transferi öncesinde flunixin meglumin (non-steroid anti inflamatuar) enjeksiyonunun gebelik oranı üzerine etkisi araştırıldı. Bu amaçla 39 baş İsviçre Esmeri düve kullanıldı. Östrüs tespitini takiben 7. günde dondurulmuş-çözdürülmüş embriyolar taşıyıcı düvelere transfer edildi. Hayvanlar rastgele iki gruba ayrıldı ve çalışma grubuna (FM grubu, n = 20) transferden 5 dak önce IM yolla 500 mg flunixin meglumin enjeksiyonu yapılırken, diğer grup (Kontrol, n = 19) kontrol olarak bırakıldı ve 2 ml fizyolojik tuzlu su solüsyonu IM olarak enjekte edildi. Bütün hayvanlara dondurulmuş-çözdürülmüş blastosist aşamasında bulunan 1. kalite embriyolar transfer edildi. Transferler çözdürmeden sonraki 5 dak içinde, üst epidural anestezi yardımıyla, corpus luteumun bulunduğu taraftaki kornu uteriye gerçekleştirildi. Gebelik muayeneleri östrüsten sonraki 30. günde ultrason kullanılarak 7.5 MHz rektal prob ile yapıldı. FM ve kontrol gruplarında gebelik oranları %50.0 ve %52.6 olarak tespit edildi. Gruplar arasındaki fark istatistiki açıdan önemli bulunmadı. Düvelerde dondurulmuş-çözdürülmüş embriyo transferinden 5 dak önce IM yolla 500 mg flunixin meglumin uygulamasının gebelik oranlarına etkisinin olmadığı kanısına varıldı.. Anahtar sözcükler: Embriyo transferi, Flunixin meglumin, Gebelik oranı The Effect of Flunixin Meglumine Injected Before Embryo Transfer on Pregnancy Rates in Heifers SummaryIn this study, the effect of flunixin meglumine (non-steroid anti-inflamatuar) injection before embryo transfer on d 7 (oestrus 0) on pregnancy rate was examined. For this aim, 39 Brown Swiss heifers were used. Following the oestrus detection, cryopreserved cattle embryos were transferred on d 7. Treatment animals were chosen randomly and injected intramuscularly 500 mg flunixin meglumine (FM group, n = 20) 5 min before embryo transfer application while the others remained as controls (Control, n = 19) and intramuscularly injected 2 ml of saline solution. All animals were transferred first quality cryopreserved blastosist embryos. Transfers were performed to uterine horn ipsilateral to the corpus luteum in 5 min after thawing with the help of epidural anesthesia. Pregnancy diagnoses were performed on d 30 by ultrasound with 7.5 MHz rectal probe. Pregnancy rates were 50.0% in FM group and 52.6% in Control group. The difference between groups was not significant. It is concluded that 500 mg flunixin meglumine injection 5 min before cryopreserved embryo transfer had no effect on pregnancy rates in heifers.

Gonadotropin-Releasing Hormone (GnRH) and Its Agonists in Bovine Reproduction II: Diverse Applications during Insemination, Post-Insemination, Pregnancy, and Postpartum Periods

“…Moreover, the administration of GnRH analogs has been suggested to improve outcomes in ET. GnRH during the diestrous phase induces the development of an accessory CL by either ovulation or luteinization of the DF in recipient cows’ ovaries [ 122 ]. A single injection of GnRH on day 5 of the estrous cycle induces ovulation and accessory CL development, resulting in higher P 4 concentrations and increased pregnancy percentages in heifers receiving in vitro-derived blastocysts [ 123 ].…”

A Critical Overview on Prostaglandin Inhibitors and Their Influence on Pregnancy Results after Insemination and Embryo Transfer in Cows

“…There was no beneficial effect of FM observed when the preparation was administered on Days 15 and 16 after timed artificial insemination (TAI), and estrus synchronized using the 5 d Co-Synch + CIDR protocol (Day 0—progesterone insert in an unknown phase of the estrus cycle, then intramuscular injection with 1 mg estradiol benzoate (EB), Day 5—injection with 400 IU of equine chorionic gonadotropin (eCG), Day 7—insert removal and intramuscular injection of 1 mg EB, Day 17—insemination) [ 32 , 33 ]. Also, Bülbül et al [ 34 ], reported that giving the recipients 500 mg FM i.m. immediately before the transfer of frozen embryos did not show a significant difference in the pregnancy rate compared to the control recipients receiving saline solution.…”

Nonsteroid Anti-Inflammatory Drugs to Improve Fertility in Cows

“…Bulbul et al [92] gave 500 mg flunixin meglumine intramuscular five minutes before embryo transfer in a study done on 39 brown Swiss. As a result of the pregnancy examination on the 30th day by means of ultrasound, they reported that pregnancy rate in the flunixin meglumine given group was lower in comparison to control group (50% vs. 52.6%).…”