The Effect of Fatal Carbon Monoxide Poisoning on the Surface Charge of Blood Cells

Szeremeta, Michał; Petelska, Aneta D.; Kotyńska, Joanna; Niemcunowicz‐Janica, Anna; Figaszewski, Zbigniew A.

doi:10.1007/s00232-013-9591-2

Cited by 12 publications

(9 citation statements)

References 25 publications

(25 reference statements)

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“…The results of the present experiment showed that CO poisoning increases the difference in RBC sizes in the blood, weakens the elastic deformation and O 2 carrying capacities of RBCs, and causes cellular hypoxia. These findings are supported by other studies (Szeremeta et al 2013). In the present study, Hb and the morphology and sizes of RBC recovered after the CO-poisoned rats were treated with HBO therapy.…”

Section: Hbo Alleviated the Hypoxia Condition In Co-poisoned Ratssupporting

confidence: 92%

The influence of hyperbaric oxygen therapy on intestinal mucosal barrier function in rats with acute carbon monoxide poisoning

Wang

Shen

Chen

et al. 2015

Toxicological & Environmental Chemistry

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Section: Hbo Alleviated the Hypoxia Condition In Co-poisoned Ratssupporting

confidence: 92%

The influence of hyperbaric oxygen therapy on intestinal mucosal barrier function in rats with acute carbon monoxide poisoning

Wang

Shen

Chen

et al. 2015

Toxicological & Environmental Chemistry

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“…Exposure to CO can lead to toxicity; it is absorbed through the lungs and reacts with the hemoglobin in red blood cells by binding to iron with 200 times higher affinity than O 2 . The complex formed is known as carboxyhemoglobin (COHb) . As most CO remains in the blood, the COHb percentage saturation (%COHb) acts as an estimate and a guide to help determine exposure levels .…”

Section: Effects Of Co In Humans According To %Cohbmentioning

confidence: 99%

Epidemiological Study of Carbon Monoxide Deaths in Scotland 2007–2016,

Lisbona

Hamnett

2018

Journal of Forensic Sciences

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Carbon monoxide (CO) intoxications are quite frequent in forensic toxicology. Using a sample of 209 CO-positive deaths in Scotland from 2007 to 2016, this study provides ranges of percentage CO saturations (%COHb) according to the CO source and examines any correlation with age, gender, alcohol, and preexisting disease. It also reports the full toxicological findings, including drug concentrations, in CO-positive cases. The highest numbers of fatalities involved males, occurred during autumn/winter, and the main source of CO was fire. The median %COHb in fire-related cases was significantly lower than in non-fire-related cases such as those involving exhausts, generators and gas supply systems, and portable BBQs. There was no relationship between %COHb and age, blood alcohol concentration, or the presence of preexisting cardiovascular and/or respiratory disease. Toxicology results revealed that prescription medications were the most commonly detected drug group and that the number of cases positive for controlled drugs was small.

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“…Сильные последствия могут возникнуть в связи с нарушениями не только газообмена, но и формы и размеров клеток. Появление HbCO может повредить основные метаболические пути, которые отвечают за нормальную функцию гемоглобина и целостность клеточной мембраны [9]. В результате это может стать причиной сближения эритроцитов и образования агрегатов (см.…”

Section: Discussionunclassified

“…Наряду с этим воздействие СО на кровь вызывает повреждение клеточной мембраны, изменяется поверхностный заряд эритроцитов [9]. К важным патогенным эффектам воздействия СО на кровь относится повышенная склонность к тромбозам [10].…”

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Changes in the morphology of erythrocytes after <i>in vitro</i> exposure of blood to carbon monoxide

Козлова¹,

Сергунова

Козлов

et al. 2019

Alʹm. klin. med.

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Background: One of the pathological effects of carbon monoxide (CO) on blood is the formation of carboxyhemoglobin. Carboxyhemoglobin completely blocks oxygen transfer; therefore, there is a net decrease in oxygen transport by red blood cells potentially resulting in tissue hypoxia. The effects of CO on blood can also damage cell membranes. Atomic force microscopy (AFM) has been recognized as effective for investigation into the mechanisms of structural damage in erythrocyte membranes. Aim: By means of AFM, to identify characteristics of changes in morphology and aggregation of erythrocytes exposed to CO in vitro.Materials and methods: All experiments were performed in vitro. We studied the morphology of erythrocytes and their aggregates with AFM. Blood sampling (150 μl) in microvettes with EDTA (Sarstedt AG & Co., Germany) was carried out during a prophylactic work-up of 5 volunteers. To obtain CO in a test tube, formic acid was mixed with sulfuric acid 1:1. Blood levels of carboxyhemoglobin were measured by spectrophotometry. A nonlinear fitting method of the experimental spectra was used to calculate the concentrations of hemoglobin derivatives in blood. Statistical analysis was done with the Origin software (OriginLab Corporation, Northampton, MA, USA).Results: After CO exposure, a shift in peaks was observed. At exposure time t₂=320 s, the percentage of carboxyhemoglobin (CHbCO) was 88±2%. As a result of blood exposure to CO, at t₁=160 s 10% of the cells differed in their shape from discocytes, whereas at t₂=320 s their proportion was 38%. With increasing duration of exposure to CO, erythrocyte aggregation occurred with formation of their large conglomerates up to 30 μm in size. In the control smear, the proportion of discocytes was 96±2%, and the remaining 4±1% of the cells had the form of echinocytes. The cell diameter (Dcont) was in the range 7.5±0.8 μm. After blood exposure to CO at t₁=160 s in the monolayer, 28±5% of cells had a diameter less than<5.7 μm. After CO exposure at t₂=320 s, the proportion of cells with a diameter of less than<5.7 μm increased to 72±11%.Conclusion: The experiments have shown that blood exposure to CO changed the morphology of erythrocytes. The formation of interconnected structures made of red blood cells was observed. With increased time of exposure, erythrocytes demonstrated aggregation with conglomerate formation.

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The Effect of Fatal Carbon Monoxide Poisoning on the Surface Charge of Blood Cells

Cited by 12 publications

References 25 publications

The influence of hyperbaric oxygen therapy on intestinal mucosal barrier function in rats with acute carbon monoxide poisoning

The influence of hyperbaric oxygen therapy on intestinal mucosal barrier function in rats with acute carbon monoxide poisoning

Epidemiological Study of Carbon Monoxide Deaths in Scotland 2007–2016,

Changes in the morphology of erythrocytes after <i>in vitro</i> exposure of blood to carbon monoxide

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