The Effect of Extracellular Matrix Molecules on the in Vitro Behavior of Bovine Endothelial Cells

Underwood, P.A.; Bennett, F.A.

doi:10.1006/excr.1993.1091

Cited by 49 publications

(33 citation statements)

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“…A study by Underwood et al [114] however, highlighted the difficulty of separating the effects of ECM proteins from the effects of cell shape on observed cell behaviour. The influence of cell shape on cell behaviour was first proposed by Folkman and Moscona [115] and subsequently it has been shown that the shape and extent of cell spreading on a surface controls cell behaviour [116].…”

Section: Ec Proliferation and Adhesion On Biomaterialsmentioning

confidence: 99%

“…When different concentrations of each ECM were used (each concentration used to achieve optimum spreading) no differences in cell morphology or growth rate were observed among different coatings. However the type and amount of ECM deposited by the cells still differed with differences in ECM substrate and time in culture [114]. This suggests that ECM composition alters cell shape, cytoskeletal organization and integrin binding.…”

Section: Ec Proliferation and Adhesion On Biomaterialsmentioning

confidence: 99%

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The influence of biomaterials on endothelial cell thrombogenicity

2007

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Driven by tissue engineering and regenerative medicine, endothelial cells are being used in combination with biomaterials in a number of applications for the purpose of improving blood compatibility and host integration. Endothelialized vascular grafts are beginning to be used clinically with some success in some centers, while endothelial seeding is being explored as a means of creating a vasculature within engineered tissues. The underlying assumption of this strategy is that when cultured on artificial biomaterials, a confluent layer of endothelial cells maintain their nonthrombogenic phenotype. In this review the existing knowledge base of endothelial cell thrombogenicity cultured on a number of different biomaterials is summarized. The importance of selecting appropriate endpoint measures that are most reflective of overall surface thrombogenicity is the focus of this review. Endothelial cells inhibit thrombosis through three interconnected regulatory systems (1) the coagulation cascade (2) the cellular components of the blood such as leukocytes and platelets and (3) the complement cascade, and also through effects on fibrinolysis and vascular tone, the latter which influences blood flow. Thus, in order to demonstrate the thromobgenic benefit of seeding a biomaterial with EC, the conditions under which EC surfaces are more likely to exhibit lower thrombogenicity than unseeded biomaterial surfaces need to be consistent with the experimental context. The endpoints selected should be appropriate for the dominant thrombotic process that occurs under the given experimental conditions.

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Section: Ec Proliferation and Adhesion On Biomaterialsmentioning

confidence: 99%

Section: Ec Proliferation and Adhesion On Biomaterialsmentioning

confidence: 99%

The influence of biomaterials on endothelial cell thrombogenicity

2007

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“…4 Therefore it is conceivable that VN is the main attachment factor adsorbing from the serum onto biomaterials. 2,3,27,33,34 Moreover, experiments with adsorption of vitronectin under denaturating conditions have shown that this protein is less sensitive (than fibronectin) to conformational changes with regard to its ability to promote cell attachment and spreading 34,35 and, in addition, it has a low tendency to desorb. 2,33 Our comparative measurements in this study performed with biotinylated VN show that comparable quantities of VN were adsorbed on hydrophobic ODS and glass from single VN solutions or serum to which labeled VN had been added.…”

Section: Discussionmentioning

confidence: 97%

“…26 Evidence from the literature suggests that VN adsorption on some biomaterial surfaces may play a greater role compared to FN with respect to attachment and spreading of anchorage dependent cells. 3,27 However, relatively little is known about the particular function and organization of the VN receptor on biomaterial surfaces. VN, together with FN, is the major cell adhesion and spreading factor existing in the plasma and in the extracellular matrix.…”

Section: Introductionmentioning

confidence: 99%

“…Adsorption of proteins, such as fibronectin (FN) and vitronectin (VN), from surrounding fluids is the first step of cellbiomaterial interaction, 1,2 followed by cell attachment and spreading. 3 Subsequently, cell growth and differentiation may be important for adoption of the implant. 4,5 It is well known that mammalian cells attach, spread, and grow better on wettable surfaces compared to hydrophobic materials.…”

Section: Introductionmentioning

confidence: 99%

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Altered vitronectin receptor (?v integrin) function in fibroblasts adhering on hydrophobic glass

Groth

Altankov

Kostadinova

et al. 1999

J. Biomed. Mater. Res.

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Function of integrins is crucial for adhesion, movement, proliferation, and survival of cells. In a recent study we found impaired fibronectin receptor function on hydrophobic substrata (G. Altankov et al. J Biomater Sci Polym Edn 1997;8:712-740). Here, we have studied the distribution and function of the vitronectin receptor (alphav integrin) in fibroblasts adhering on hydrophilic glass and hydrophobic octadecyl glass (ODS). The morphology of fibroblasts and the organization of actin cytoskeleton were studied and found to be altered on ODS, where the cells did not spread and possessed condensed actin. Pretreatment of the surfaces with serum or pure vitronectin improved cell morphology on both substrata, resulting in the development of longitudinal actin stress fibers. It was found with biotinylated vitronectin that comparable quantities of vitronectin were adsorbed from single vitronectin solutions or serum on glass and on hydrophobic ODS. The organization of the vitronectin receptors on the ventral cell surface was investigated in permeabilized cells showing normal focal adhesions in fibroblasts plated on glass but none of these structures on ODS. The distribution of alphav integrin on the dorsal cell surface was studied on nonpermeabilized living cells after antibody tagging. While fibroblasts adhering on plain or serum-treated glass developed a linear organization of alphav integrin, cells on plain and serum-treated ODS were not able to reorganize the vitronectin receptor. Studies on signal transduction with antiphosphotyrosine antibodies revealed co-localization of alphav integrin and phosphotyrosine in focal adhesions on glass and serum-treated glass. However, signaling was almost absent on plain ODS and weak on serum-treated ODS. It was concluded that alterations in vitronectin receptor function on the ventral cell surface caused by the hydrophobic material surface inhibit signal transfer and subsequent intracellular events that are important for the organization and function of integrins.

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Bioactive surface coatings for nanoscale instruments: Effects on CNS neurons

Ignatius

Sawhney

Gupta

et al. 1998

J. Biomed. Mater. Res.

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A method is described for depositing onto medical instruments highly biocompatible and bioactive surface coatings that can promote and stabilize cell attachment. The coatings were made by first depositing thin films of materials, such as diamond-like carbon, or metals, including tantalum, tungsten, platinum, gold, iridium, palladium, and brass. These surfaces were further altered to either promote or inhibit cell growth and spreading by an additional overcoat of biological materials, including the extracellular matrix proteins, laminin, fibronectin, and collagen IV. The deposition technique used a metal or carbon plasma, and the important properties of film adhesion, hardness, density, and smoothness are tailored by control of the ion bombardment energy. The films are translucent enough to permit high resolution light microscopy for rapid and detailed examination of tissue response. These bioactive substrates have been tested on primary central nervous system neurons, and the growth response is excellent. Equally successful have been our attempts to anchor neurons, without associated proliferation of non-neuronal cells, using coatings of poly-d-lysine. The method and the materials could have important ramifications in a number of areas of research and biotechnology, for example for chronic implantation of microelectrode arrays in the cerebral cortex for neuroprosthetic and neural monitoring application and for research on the human central nervous system. Possible application in nonneuronal fields, such as for coronary artery stents and pacemaker electrodes, also are discussed.

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The Effect of Extracellular Matrix Molecules on the in Vitro Behavior of Bovine Endothelial Cells

Cited by 49 publications

References 0 publications

The influence of biomaterials on endothelial cell thrombogenicity

The influence of biomaterials on endothelial cell thrombogenicity

Altered vitronectin receptor (?v integrin) function in fibroblasts adhering on hydrophobic glass

Bioactive surface coatings for nanoscale instruments: Effects on CNS neurons

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