The effect of external electron beam on neointima in rat carotid artery injury model

Oh, Young‐Taek; Kim, Han‐Soo; Chun, Mison; Kang, Hyein; Yoon, Myeong‐Ho; Kim, Jung-Sun; Kang, Sung Soo; Joh, Chul-Woo; Kim, Youngmi; Choi, Byung-Il; Park, Kyung Bae; Park, Chan Hee

doi:10.1016/s0360-3016(98)00551-3

Cited by 8 publications

(2 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Also, in a study using LDL receptor knockout mice, total body irradiation and donor bone marrow transplantation did not accentuate the progression of atherosclerosis, even though the dosage of external total body irradiation employed was higher than that in the current study [18]. Moreover, Oh et al [19] reported that an external electron beam reduces intimal area in the rat carotid injury model rather than increasing it [19]. It thus seems likely that mechanical damage is the most important factor in determining the type of healing response of arteries in the current studies, rather than the irradiation and transfusion with male marrow.…”

Section: Discussionmentioning

confidence: 58%

Circulating Bone Marrow Cells Can Contribute to Neointimal Formation

Han

Campbell²,

Campbell³

2001

J Vasc Res

208

137

View full text Add to dashboard Cite

To examine the source of smooth muscle-like cells during vascular healing, C57BL/6 (Ly 5.2) female mice underwent whole body irradiation followed by transfusion with 10⁶ nucleated bone marrow cells from congenic (Ly 5.1) male donors. Successful repopulation (88.4 ± 4.9%) by donor marrow was demonstrated in the female mice by flow cytometry with FITC-conjugated A20.1/Ly 5.1 monoclonal antibody after 4 weeks. The arteries of the female mice were then subjected to two types of insult: (1) The iliac artery was scratch-injured by 5 passes of a probe causing severe medial damage. After 4 weeks, the arterial lumen was obliterated by a cell-rich neointima, with cells containing α smooth muscle actin present around the residual lumen. Approximately half of these cells were of male donor origin, as evidenced by in situ hybridization with a Y-chromosome-specific probe. (2) In an organized arterial thrombus formed by inserting an 8-0 silk suture into the left common carotid artery, donor cells staining with α smooth muscle actin were found in those arteries sustaining serious damage but not in arteries with minimal damage. Our results suggest that bone marrow-derived cells are recruited in vascular healing as a complementary source of smooth muscle-like cells when the media is severely damaged and few resident smooth muscle cells are available to effect repair.

show abstract