The Effect of Exposure Time on Maximum Acceptable Dose for Avoidance of Ultraviolet Radiation-Induced Cataract

Dong, Xiaofang; Söderberg, Per G.; Ayala, Marcelo; Löfgren, Stefan

doi:10.1159/000086596

Cited by 9 publications

(4 citation statements)

References 22 publications

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“…All lenses were irradiated with a dosage of 6 kJ/cm 2 . This high dosage was chosen according to other experiments inducing cataracts with UVB (about 300 nm) radiation with a dosage of 0.8 J/cm 2 [29]. The biochemical impact of blue light is about four orders of magnitude smaller than UVB radiation's impact [42].…”

Section: Methodsmentioning

confidence: 99%

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Cataract Development by Exposure to Ultraviolet and Blue Visible Light in Porcine Lenses

2021

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Background and Objectives: Cataract is still the leading cause of blindness. Its development is well researched for UV radiation. Modern light sources like LEDs and displays tend to emit blue light. The effect of blue light on the retina is called blue light hazard and is studied extensively. However, its impact on the lens is not investigated so far. Aim: Investigation of the impact of the blue visible light in porcine lens compared to UVA and UVB radiation. Materials and Methods: In this ex-vivo experiment, porcine lenses are irradiated with a dosage of 6 kJ/cm2 at wavelengths of 311 nm (UVB), 370 nm (UVA), and 460 nm (blue light). Lens transmission measurements before and after irradiation give insight into the impact of the radiation. Furthermore, dark field images are taken from every lens before and after irradiation. Cataract development is illustrated by histogram linearization as well as faults coloring of recorded dark field images. By segmenting the lens in the background’s original image, the lens condition before and after irradiation could be compared. Results: All lenses irradiated with a 6 kJ/cm2 reveal cataract development for radiation with 311 nm, 370 nm, and 460 nm. Both evaluations reveal that the 460 nm irradiation causes the most cataract. Conclusion: All investigated irradiation sources cause cataracts in porcine lenses—even blue visible light.

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Section: Methodsmentioning

confidence: 99%

“…Studies and Experiments. Most research regarding in vivo experiments is performed with rodents, especially rats [19,24,[28][29][30][31][32][33]. The irradiation dosage needed to induce cataracts in rat eyes with a wavelength of 300 nm is determined to be 2.2 kJ/m 2 [32].…”

Section: Introductionmentioning

confidence: 99%

Cataract Development by Exposure to Ultraviolet and Blue Visible Light in Porcine Lenses

2021

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“…Dieser Zusammenhang gilt nur für photochemische Prozesse in leblosen Stoffen. Das photochemische Reziprozitätsge-setz gilt nicht für photobiologische Prozesse im Fall von kurzen Bestrahlungszeiten, weil das biologische Gewebe über eine bestimmte Schutzkapazität (Radikalfänger) gegen photochemische Reaktionen verfügt [4,11]. Im Anfangszeitraum besteht ein Gleichgewicht zwischen der Radikalbildung und den biologischen Schutzmechanismen.…”

Section: Der Absorptionskoeffizient Und Damit Die Konzentration Dür-unclassified

Biophysikalische Grundlagen der Kollagenvernetzung

Spörl

Raiskup-Wolf²,

Pillunat³

2008

Klin Monatsbl Augenheilkd

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“…Five minutes after pupillary dilation, one eye from each animal was exposed to a total dose of 1 kJ/m 2 UVR‐300 nm for 15 min (Dong et al. ). The 1 kJ/m 2 UVR‐300 nm corresponds to one‐third of the maximum tolerance dose (MTD 2.316 ) in rats (Söderberg et al.…”

Section: Methodsmentioning

confidence: 99%

Time evolution of active caspase‐3 labelling after in vivo exposure to UVR‐300 nm

Talebizadeh

Kronschläger

et al. 2014

Acta Ophthalmologica

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ABSTRACT.Purpose: To determine the time evolution of active caspase-3 protein expression in albino rat lens after in vivo exposure to low-dose UVR-300 nm, as detected by immunofluorescence. Methods: Forty Sprague-Dawley rats were unilaterally exposed in vivo to 1 kJ/ m 2 UVR-300 nm for 15 min. At 0.5, 8, 16 and 24 hr after the UVR exposure, the exposed and contralateral nonexposed lenses were removed and processed for immunohistochemistry. Three mid-sagittal sections from each lens were stained. The cells labelled for active caspase-3 in each section of both the exposed and nonexposed lenses were counted and recorded three times. The difference of the proportion of labelling between the exposed and contralateral nonexposed lenses within each animal was calculated. The differences of active caspase-3 labelling at four different time-points after exposure were used to determine the time evolution of active caspase-3 expression. Results: Caspase-3 expression was higher in the exposed than in contralateral nonexposed lenses. The mean difference between the exposed and contralateral nonexposed lenses, including all lenses from all time intervals, was 0.12 AE 0.01 (= CI 95%). The mean differences between the exposed and contralateral nonexposed lenses were 0.11 AE 0.02, 0.13 AE 0.02, 0.14 AE 0.01 and 0.09 AE 0.03 (= CI 95%) for the 0.5-, 8-, 16-and 24-hr time groups, respectively. The orthogonal comparison showed no difference in the expression of active caspase-3 between the 0.5-and the 24-hr groups (Test statistic 1.50, F 1,36 = 4.11, p < 0.05) or between the 8-and the 16-hr groups (test statistic 0.05, F 1,36 = 4.11, p < 0.05). There was a difference when comparing the 0.5-and 24-hr groups to the 8-and 16-hr groups (test statistic 7.01, F 1,36 = 4.11, p < 0.05). Conclusion: The expression of active caspase-3 in the lens epithelium increases after UVR exposure. There is a peak of expression approximately 16 hr after the exposure.

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The Effect of Exposure Time on Maximum Acceptable Dose for Avoidance of Ultraviolet Radiation-Induced Cataract

Cited by 9 publications

References 22 publications

Cataract Development by Exposure to Ultraviolet and Blue Visible Light in Porcine Lenses

Cataract Development by Exposure to Ultraviolet and Blue Visible Light in Porcine Lenses

Biophysikalische Grundlagen der Kollagenvernetzung

Time evolution of active caspase‐3 labelling after in vivo exposure to UVR‐300 nm

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