The Effect of Experimental Varicocele on the Testis of Adolescent Rats

Choi, Hwang; Kim, Kun Suk; Kim, Kwang Myung

doi:10.1016/s0022-5347(17)39502-2

Cited by 21 publications

(25 citation statements)

References 17 publications

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“…It is estimated that approximately one third of the men evaluated for infertility have this condition [1]. The literature indicates that 8% to 23% of men have clinically evident varicocele [2].…”

Section: Introductionmentioning

confidence: 99%

The Effect of the Partial Obstruction Site of the Renal Vein on Testis and Kidney in Rats: Is the Traditional Animal Model Suitable for Varicocele Research?

Chae

Kim

et al. 2010

Korean J Urol

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PurposeWe investigated the influence of the location of the partial renal vein obstruction on the left kidney, the bilateral testes, and cauda epididymal sperm quality and determined whether this animal model is suitable for varicocele study.Materials and MethodsA total of 25 adult male Sprague-Dawley rats were assigned to three groups: group 1 (experimental varicocele by partial ligation medial to the internal spermatic vein for 8 weeks, n=8), group 2 (partial ligation lateral to the internal spermatic vein for 8 weeks, n=10), and group 3 (sham operation for 8 weeks, n=7). Rats in groups 1, 2, and 3 underwent a left nephrectomy and bilateral orchiectomy at 8 weeks after the operation. Histological changes and Johnsen score in both testes were analyzed. Fibrotic changes in the left kidney were assessed by quantitative image analysis. Numbers of sperm and proportions of motile sperm in the cauda epididymides were determined.ResultsSignificant histological abnormalities and Johnsen score changes were observed in the testes in group 1. Renal fibrosis did not differ significantly among the groups. The proportions of motile sperm were significantly lower bilaterally in group 1 than in groups 2 and 3. However, the mean bilateral epididymal sperm count in group 1 was not significantly lower than in groups 2 and 3.ConclusionsOur results showed that experimental varicocele in the rat, induced by partial ligation medial to the internal spermatic vein, influences epididymal sperm quality without harmful effects on the left kidney. The present study certifies that this traditional animal model is suitable for varicocele research.

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Section: Introductionmentioning

confidence: 99%

The Effect of the Partial Obstruction Site of the Renal Vein on Testis and Kidney in Rats: Is the Traditional Animal Model Suitable for Varicocele Research?

Chae

Kim

et al. 2010

Korean J Urol

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“…An explanation is that histological changes in testes caused by varicocele are generally focal and fertility is mostly affected if these changes are diffuse in both testes [1,2] . In this study, ␥ -H2AX immunolocalization was still detected in the spermatogonia 4 weeks after varicocele creation, while the primary spermatocytes underwent apoptotic changes.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, the thickness of the seminiferous tubule is inversely related to the duration of varicocele [1][2][3][4] . Oxidative stress by reactive oxygen species (ROS) plays a role in the development of varicocele [5,6] .…”

Section: Introductionmentioning

confidence: 99%

Experimental Varicocele Induces p53-Dependent Germ Cell Apoptosis through Activation of γ-H2AX

Chang

Kim²,

Park³

et al. 2010

Urol Int

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Aim: Since DNA damage-related apoptosis is raising concerns regarding abnormal spermatogenesis, we investigated the changes in γ-H2AX during testicular germ cell apoptotic responses in the varicocele model. Materials and Methods: Varicocele was induced by partial ligation of the left renal vein and animals were sacrificed at 1, 3, and 4 weeks after varicocele creation. The levels of activated p53 and γ-H2AX formation were determined by Western blot analysis and immunohistochemistry. Results: γ-H2AX formation was augmented after varicocele creation, while a significant increase in p53 phosphorylation was detected in a time course-dependent manner. Varicocele-dependent nuclear γ-H2AX staining in the primary spermatocytes was prominent as degenerative foci, while little differences could be detected in spermatogonia. Conclusions: These results show that experimental varicocele may induce p53-dependent apoptosis through activation of γ-H2AX in the primary spermatocytes, and suggest that γ-H2AX may be related to apoptotic signal transduction in experimental varicocele.

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“…Atrophy or hypotrophy in comparison with the contralateral testis has been proposed as an indication for treatment (2). Experimental left varicocele was reported not to influence testicular weight in adult rats, but to cause significant weight and histologic changes in the testes of adolescent rats (2), and early treatment in human adolescents has therefore been strongly recommended (6, 10,12,14). Although the condition is common, its pathophysiologic effects on the testes remain enigmatic.…”

Section: Discussionmentioning

confidence: 99%

Testicular Size Following Embolization Therapy for Paediatric Left Varicocele

Rivilla

Casillas

1997

Scandinavian Journal of Urology and Nephrology

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Optimal management of varicocele testis in children and adolescents is controversial, but testicular hypotrophy has been suggested as an indication for treatment in adolescents. This report concerns the diagnosis and management of left varicocele in 30 patients aged 6-15 years and the influence of treatment on testicular size. The left testicle was significantly smaller than the right in 14 patients at enrollment in the study. All patients were treated in local anaesthesia with percutaneous transfemoral spermatic venography and embolization of that vein with spring coil, followed by 24-hour observation. There were no major complications. At follow-up 9 to 12 months after the treatment, the size of the left testis had increased in 12 patients. Spring-coil embolization of the spermatic vein proved to be a safe and effective technique for treatment of varicocele.

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The Effect of Experimental Varicocele on the Testis of Adolescent Rats

Cited by 21 publications

References 17 publications

The Effect of the Partial Obstruction Site of the Renal Vein on Testis and Kidney in Rats: Is the Traditional Animal Model Suitable for Varicocele Research?

The Effect of the Partial Obstruction Site of the Renal Vein on Testis and Kidney in Rats: Is the Traditional Animal Model Suitable for Varicocele Research?

Experimental Varicocele Induces p53-Dependent Germ Cell Apoptosis through Activation of γ-H2AX

Testicular Size Following Embolization Therapy for Paediatric Left Varicocele

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