The effect of exogenous histone H1 on rat adipose‐derived stem cell proliferation, migration, and osteogenic differentiation in vitro

Abstract

Adipose-derived stem cells (ASCs) are of great interest for the development of novel cell therapies due to their ease of isolation and expansion, immunosuppressive activity, and multilineage differentiation potential. However, the mechanisms underlying the therapeutic potential of ASCs remain to be elucidated. Others and we have shown that nuclear proteins such as histone H1 and high mobility group box 1 (HMGB1) play important roles in the maturation of dendritic cells (DCs). Furthermore, we previously demonstrated translocation of histone H1 from the nucleus to the cytoplasm and activation of mitogen-activated protein kinases (MAPKs) in DCs. In the present study, we confirmed that histone H1 does not alter the immunophenotype and immunosuppression potential of ASCs, but that histone H1 enhanced wound healing and increased interleukin (IL)-6 expression. Moreover, histone H1 treated-ASCs showed up-regulation of MAPKs extracellular-regulated kinase 1/2 (ERK1/2) and sequential NF-κB translocation. Finally, we found that culture in differentiation media supplemented with histone H1 enhanced ASC osteogenesis. In contrast, inhibition of histone H1 by small interfering RNA (siRNA) reduced osteogenic differentiation markers including ALP. These results suggest that histone H1 may be useful for induction of mesenchymal stem cells in tissue engineering and future potential ASC therapies.

Local delivery of HMGB1 in gelatin sponge scaffolds combined with mesenchymal stem cell sheets to accelerate fracture healing

“…Hsu et al [11] found that HMGB1 promotes the osteogenic differentiation of adipose-derived stem cells and activates the ERK1/2 signal pathway, but the role of the latter in mediating the effects of HMGB1 on osteogenic differentiation was not determined. Our previous study showed that HMGB1 activates the MAPK signal pathway to promote the osteogenic differentiation of MSCs [12, 13].…”

“…Recently, HMGB1 has been shown to promote osteogenesis. Hsu et al [11] reported that HMGB1 promoted the osteogenesis of adipose tissue-derived stem cells. We earlier showed that HMGB1 stimulated MSC osteogenesis and migration [12, 13].…”

Hox genes are involved in vascular wall-resident multipotent stem cell differentiation into smooth muscle cells

“…The knockdown of H1.0 in human ES did not affect self-renewal but impaired their differentiation. Moreover, H1 has been shown to enhance wound healing and increase interleukin -6 expressions of adult stem cells42. Thereby, H1 treatment showed an up-regulation of mitogen-activated protein kinases (MAPKs), extracellular-regulated kinase 1/2 (ERK1/2) and sequential NF-κB translocation.…”

The effect of adipose derived stromal cells on oxidative stress level, lung emphysema and white blood cells of guinea pigs model of chronic obstructive pulmonary disease

“…The cells were maintained in the differentiation medium for two weeks and the culture medium was replaced every 3 days [21]. For Alizarin red staining, the cells were fixed with 10% formalin and then incubated with 2% Alizarin red solution.…”