The effect of adipose derived stromal cells on oxidative stress level, lung emphysema and white blood cells of guinea pigs model of chronic obstructive pulmonary disease

Ghorbani, Ahmad; Feizpour, Azadeh; Hashemzahi, Milad; Gholami, Lila; Hosseini, Mahmoud; Soukhtanloo, Mohammad; Bagheri, Farzaneh; Khodaei, Esmaeil; Roshan, Nema Mohamadian; Boskabady, Mohammad Hossein

doi:10.1186/2008-2231-22-26

Cited by 27 publications

(20 citation statements)

References 32 publications

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“…Increased interalveolar septum, presence of lymphatic tissue in the lung parenchyma, destruction of alveolar wall, and existence of emphysema in the lung and intra-alveolar bleeding of lungs were also observed in guinea pig models of COPD following exposure to CS [50, 51, 54–57]. In another study, airway and parenchymal neutrophilia, increased goblet cell numbers, lung hydroxyproline content, airway wall collagen and airspace size, were reported [69].…”

Section: Measured Parametersmentioning

confidence: 97%

“…Total WBC [51] and eosinophil counts in blood were increased in guinea pig models of CS-induced COPD [50, 53]. Total WBC, eosinophils and neutrophils in lung lavage of COPD guinea pigs were also increased [52].…”

Section: Inflammatory Cells and Mediatorsmentioning

confidence: 99%

“…Increased serum levels of IL-8 and malondialdehyde (MDA) in guinea pigs model of COPD induced by CS, were reported [50, 51, 53]. In addition, Feizpour et al indicated that the level of IL-8 in serum and BAL of guinea pig models of CS-induced COPD, were increased [52].…”

Section: Inflammatory Cells and Mediatorsmentioning

confidence: 99%

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Experimental animal models for COPD: a methodological review

Ghorani¹,

Boskabady²,

Khazdair³

et al. 2017

Tob. Induced Dis.

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164

111

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IntroductionChronic obstructive pulmonary disease (COPD) is a progressive disorder that makes the breathing difficult and is characterized by pathological conditions ranging from chronic inflammation to tissue proteolysis. With regard to ethical issues related to the studies on patients with COPD, the use of animal models of COPD is inevitable. Animal models improve our knowledge about the basic mechanisms underlying COPD physiology, pathophysiology and treatment. Although these models are only able to mimic some of the features of the disease, they are valuable for further investigation of mechanisms involved in human COPD.MethodsWe searched the literature available in Google Scholar, PubMed and ScienceDirect databases for English articles published until November 2015. For this purpose, we used 5 keywords for COPD, 3 for animal models, 4 for exposure methods, 3 for pathophysiological changes and 3 for biomarkers. One hundred and fifty-one studies were considered eligible for inclusion in this review.ResultsAccording to the reviewed articles, animal models of COPD are mainly induced in mice, guinea pigs and rats. In most of the studies, this model was induced by exposure to cigarette smoke (CS), intra-tracheal lipopolysaccharide (LPS) and intranasal elastase. There were variations in time course and dose of inducers used in different studies. The main measured parameters were lung pathological data and lung inflammation (both inflammatory cells and inflammatory mediators) in most of the studies and tracheal responsiveness (TR) in only few studies.ConclusionThe present review provides various methods used for induction of animal models of COPD, different animals used (mainly mice, guinea pigs and rats) and measured parameters. The information provided in this review is valuable for choosing appropriate animal, method of induction and selecting parameters to be measured in studies concerning COPD.

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Section: Measured Parametersmentioning

confidence: 97%

Section: Inflammatory Cells and Mediatorsmentioning

confidence: 99%

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Experimental animal models for COPD: a methodological review

Ghorani¹,

Boskabady²,

Khazdair³

et al. 2017

Tob. Induced Dis.

Self Cite

164

111

View full text Add to dashboard Cite

show abstract

“…In the present study, we used cigarette smoke exposure to induce of animal model of COPD. Several previous studies have shown that consecutive cigarette smoke exposure can successfully induce the COPD in rats [7][8][9] . This study clearly demonstrated that consecutive induction of cigarette smoke can lead to the significantly distinctive change of COPD and lung inflammatory in rats.…”

Section: Table2 the Results Of Total Wbc Numbers And The Percentage mentioning

confidence: 99%

Compound injection of Danshen root suppresses cigarettes smoking-induced lung inflammation: a SD rat model

2015

Inflamm Cell Signal

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Background:To investigate the therapeutic property for Danshen in the development of COPD using SD rats as animal model. Methods: a total of 30 rats were consecutively exposed in cigarette smoke for three months, and the Bronchoalveolar lavage fluid (BALF) was collected followed sacrifice. These rats were randomly divided five groups determined by the approaches of treatment (COPD group: smoke+ Sodium Chloride 0.2ml/100g; Control: only exposed to ambient air+ Sodium Chloride 0.2ml/100g; A, B and C groups: smoke+Danshen 0.1, 0.2, 0.4 ml/100g respectively). The level of IL-6, IL-8 and TNF-α, and the total white blood cell (WBC) and percentage of differential WBC were measured. Results: In contrast with the sections from COPD group, lesions in lung were slighter in A, B and C groups. Compared with control group, the levels of IL-8, IL-6, TNF-α gradually decreased from COPD, A and B group. However, no differences on IL-8, IL-6, TNF-α were detected between B and C group (all P values was larger than 0.05). Conclusion: Danshen showed a preventive therapeutic potential on lung inflammation

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“…The tissue was minced into small pieces and incubated for 40 min in PBS containing 2 mg/ml collagenase at 37°C with mild agitation [21,22]. After centrifuging, the floated adipocytes were discarded and the stromal cells were suspended in DMEM medium supplemented with 10% fetal bovine serum, 100 U/ml penicillin and 100 μg/ml streptomycin and cultured in flask.…”

Section: Methodsmentioning

confidence: 99%

Metabolic effects of newly synthesized phosphodiesterase-3 inhibitor 6-[4-(4-methylpiperidin-1-yl)-4-oxobutoxy]-4-methylquinolin-2(1H)-one on rat adipocytes

et al. 2015

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BackgroundClinical use of selective PDE3 inhibitors as cardiotonic agents is limited because of their chronotropic and lipolytic side effects. In our previous work, we synthesized a new PDE3 inhibitor named MC2 (6-[4-(4-methylpiperidin-1-yl)-4-oxobutoxy]-4-methylquinolin-2(1H)-one) which produced a high positive inotropic action with a negative chronotropic effect. This work was done to evaluate the effects of MC2 on adipocytes and compare its effects with those of amrinone and cilostamide.MethodsPreadipocytes were isolated from rat adipose tissue and differentiated to adipocyte in the presence of cilostamide, amrinone or MC2. Lipolysis and adipogenesis was evaluated by measuring glycerol level and Oil Red O staining, respectively. Adipocyte proliferation and apoptosis were determined with MTT assay and Annexin V/PI staining, respectively.ResultsDifferentiation to adipocyte was induced by amrinone but not by cilostamide or MC2. Basal and isoproterenol-stimulated lipolysis significantly increased by cilostamide (p < 0.05). Similarly, amrinone enhanced the stimulated lipolysis (p < 0.01). On the other hand, MC2 significantly decreased both adipogenesis (p < 0.05) and stimulated lipolysis (p < 0.001). Also, incubation of differentiated adipocytes with MC2 caused the loss of cell viability, which was associated with the elevation in apoptotic rate (p < 0.05).ConclusionOur data indicate that selective PDE3 inhibitors produce differential effects on adipogenesis and lipolysis. MC2 has proapoptotic and antilipolytic effects on adipocytes and does not stimulate adipogenesis. Therefore, in comparison with the clinically available selective PDE3 inhibitors, MC2 has lowest metabolic side effects and might be a good candidate for treatment of congestive heart failure.

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The effect of adipose derived stromal cells on oxidative stress level, lung emphysema and white blood cells of guinea pigs model of chronic obstructive pulmonary disease

Cited by 27 publications

References 32 publications

Experimental animal models for COPD: a methodological review

Experimental animal models for COPD: a methodological review

Compound injection of Danshen root suppresses cigarettes smoking-induced lung inflammation: a SD rat model

Metabolic effects of newly synthesized phosphodiesterase-3 inhibitor 6-[4-(4-methylpiperidin-1-yl)-4-oxobutoxy]-4-methylquinolin-2(1H)-one on rat adipocytes

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