THE EFFECT OF ENVIRONMENTAL ANEUPLOIDY‐INDUCING AGENTS ON THE MICROTUBULE ARCHITECTURE OF MITOTIC MERISTEMATIC ROOT CELLS INHORDEUM VULGARE

Abstract

Microtubules are the prime components involved in chromosomal segregation, their functional accuracy ensuring maintenance of the normal karyotype in the progeny. Chemically-induced disruption of microtubules during mitosis can lead to aneuploidy. In this study, seven environmental chemicals, i.e. cadmium chloride (CD), econazole nitrate (EZ), benomyl (BM), thiabendazole (TB), griseofulvin (GF), thimerosal (TM) and hydroquinone (HQ), were tested for their ability to induce microtubule disruption in mitotic meristematic root cells of the higher plant Hordeum vulgare, with the use of anti-tubulin indirect immunofluorescence microscopy. All chemicals tested in this study, with the exception of TB and HQ, produced modifications in the morphology of microtubule organization and reduced the fidelity of the spindle apparatus in Hordeum vulgare.

Contribution to the validation of the anaphase-telophase test: aneugenic and clastogenic effects of cadmium sulfate, potassium dichromate and nickel chloride in Chinese hamster ovary cells

“…Like another aneuploidy inducers, cadmium chloride modifies the microtubular organization and reduces the fidelity of the splindle apparatus in Hordeum vulgare (Voutsinas et al, 1997). Chromium (VI) readily enters cell through non-specific anion channels and reactive intermediates formed during intracellular Cr (VI) reduction may be responsible for some of this compound's genotoxicity (Biedermann and Landolph, 1990;De Flora et al, 1990;Katz and Salem, 1993).…”

Effect of taxol and okadaic acid on microtubule dynamics in thimerosal‐arrested primary mouse oocytes: a confocal study

“…Considering the well documented sensitivity of the polymerisability of tubulin to blocking of sulfhydryl groups (Ikeda and Steiner, 1978;Brunner et al, 1991;Roychaudhuri and Biswas, 1992;Voutsinas et al, 1997), we evaluated the cytological effects of the inhibitory 10 min-long treatment with TMS 0.4 mM using confocal fluorescence microscopy. A special attention was thus paid to the microtubule network especially since this might contribute to the study of the interplay between microtubules and the cell cycle control machinery in mouse oocytes .…”

Avaliação do potencial genotóxico e mutagênico de amostras de águas de recursos hídricos que recebem efluentes urbanos e industriais do pólo ceramista da cidade de Santa Gertrudes - SP

“…Vem sendo desenvolvidos diversos testes com Allium cepa, dentre eles os de toxicidade (FISKESJÖ, 1985;PALACIO et al, 2005;BARBERIO et al, 2009;GUERRA, 2009), de genotoxicidade e mutagenicidade (FISKESJÖ, 1985;NIELSEN, 1994;FATIMA;AHMAD, 2006;MIGID et al, 2007;CARITÁ;GANA et al, 2008;VUJOŠEVIÉ et al, 2008;HOSHINA et al, 2009;RADIC et al, 2010). Células meristemáticas de A. cepa são indicadas para a determinação de efeitos citotóxicos, genotóxicos e mutagênicos de diferentes substâncias (GRANT, 1982;FISKESJÖ, 1985; MATSUMOTO; MARIN-MORALES, 2004;CARITÁ;MAZZEO, 2009;ROBERTO, 2009), caracterizando-se um material indicado para análises de poluição de recursos hídricos pela sensibilidade na avaliação da qualidade da água (SMAKA-KINCL et al, 1996, RADIC et al, 2010.…”

“…Como exemplo, pode-se citar os estudos realizados com os rios Pó, Ticino e Lambro (Itália) , o rio Noyyal (Índia) , o rio Paraíba do Sul (Brasil) (SOUZA;, o rio Guaecá (Brasil) , o rio Rasina (Sérvia) (VUJOŠEVIÉ et al, 2008), o rio Atibaia (Brasil) ( HOSHINA et al, 2009); o Rio Tietê (Brasil) , o rio Sava (Croácia) (RADIC et al, 2010), o córrego dos Cristais (Brasil) (CARITÁ; , as lagoas da Turquia (Akgol e Paradeniz do Delta Goksu) , o Estuário Sado (Portugal) (COSTA et al, 2008) e águas do mar próximas à Estação Brasileira Comandante Ferraz na Antártida (NGAN et al, 2007).…”