The Effect of Endophytic Bacteria Bacillus subtilis and Salicylic Acid on Some Resistance and Quality Traits of Stored Solanum tuberosum L. Tubers Infected with Fusarium Dry Rot

Abstract

The effect of endophytic Bacillus subtilis (strains 10-4, 26D) and their compositions with salicylic acid (SA) on some resistance and quality traits of stored potatoes infected with Fusarium dry rot were studied. The experiments were carried out on hydroponically grown Solanum tuberosum L. tubers that were infected before storage with Fusarium oxysporum and coated with B. subtilis 10-4, 26D with and without exogenous SA, and then stored for six months. It has been shown that 10-4, 26D, 10-4 + SA, and 26D + SA reduced in different levels (up to 30–50%) the incidence of F. oxysporum-caused dry rot (with the highest effect for 10-4 + SA). SA notably enhanced the positive effect of 10-4, while for 26D, such an effect was not observed. All of the tested treatments increased amylase (AMY) and AMY inhibitors activity in infected tubers, while decreased Fusarium-induced protease activity (except in the case of 10-4 + SA, which promoted a slight increase) was revealed. 10-4, 26D, and their compositions with SA decreased (in different degrees) the pathogen-caused lipid peroxidation, proline, and reducing sugars accumulation in potatoes after long-term storage. It was also discovered 10-4 and 26D, regardless of SA presence, decrease pathogen-induced glycoalkaloids α-Solanine and α-Chaconine accumulation and preserved increased levels of starch and total dry matter in infected stored potatoes. The findings indicate endophytic B. subtilis and its compositions with SA is a promising eco-friendly and bio-safe approach to cope with postharvest decays of potato during long-term storage; however, when developing preparations-compositions it should take into account the strain-dependent manner of B. subtilis action together with SA.

Dual inoculation of Bradyrhizobium and Enterobacter alleviates the adverse effect of salinity on Glycine max seedling

“…H2O2 is responsible for the increased solute degradation related to oxidative stress (Silva et al, 2020;Mohamed et al, 2012Mohamed et al, ,2016. Lastochkina et al (2020) noted the pattern of clarification close to our observation. Dual inoculation with B. japonicum and Delta PSK can be regarded as a promising source for preserving the integrity of the plasma membrane and regulating water intake, and increasing water use efficiency, thus minimizing oxidative stress (Harman et al, 2019).…”

Antimicrobial Activity Against Phytopathogens and Inhibitory Activity on Solanine in Potatoes of the Endophytic Bacteria Isolated From Potato Tubers

“…The results revealed that most of the efficient solanine-degrading strains belonged to the genus Arthrobacter ( Hennessy et al, 2018 ). The other study showed that Bacillus subtilis could regulate the solanine content in healthy and Fusarium -infected tubers of potatoes during storage ( Lastochkina et al, 2020 ). However, the mechanisms of endophytic bacteria regulated the content of solanine were still unclear, which needed further research.…”

Genomic Analysis and Secondary Metabolites Production of the Endophytic Bacillus velezensis Bvel1: A Biocontrol Agent against Botrytis cinerea Causing Bunch Rot in Post-Harvest Table Grapes

“…Endophytic Bacillus may also be more likely to succeed as biocontrol agents as a result of their environmental adaptation to host plant metabolites and their ability to occupy ecological niches similar to fungal pathogens, such as B. cinerea, that exhibit a latent or necrotrophic behavior [ 18 ]. Recent studies exploring the biocontrol capacity of endophytic Bacillus strains against post-harvest pathogens have demonstrated the successfully suppression of the proliferation of Fusarium dry rot in potato [ 19 ], B. cinerea in tomato [ 20 ], and Colletotrichum acutatum in banana [ 21 ].…”