The effect of endodontic materials on the optical density of dyes used in marginal leakage studies

Abstract

The aim of this study was to determine the effect of the exposure of different endodontic materials to different dye solutions by evaluating the optical density of the dye solutions. Seventy-five plastic tubes were filled with one of the following materials: AH Plus, Sealapex, Portland cement, MTA (Angelus and Pro Root) and fifteen control plastic tubes were not. Each specimen of material and control was immersed in a container with 1 ml of each dye solution. A 0.1 ml-dye solution aliquote was removed before immersion and after 12, 24, 48 and 72 hours of each specimen immersion to record its optical density (OD) in a spectrophotometer. Statistical analysis was performed with ANOVA and Tukey tests (5%). No significant difference was found among any of the solution OD values for AH Plus cement. Portland cement promoted different OD values after 12 hours of immersion. MTA-Angelus cement presented different OD values only for 2% rhodamine B and the MTAPro Root cement presented different OD values in all 2% rhodamine B samples. Sealapex cement promoted a reduction in the India Ink OD values. Dye evaluation through OD seems to be an interesting method to select the best dye solution to use in a given marginal leakage study.

Effectiveness of different final irrigation techniques and placement of endodontic sealer into dentinal tubules

“…24 Rhodamine B isothiocyanate is a fluorescent dye with chemical formula C 29 H 30 ClN 3 O 3 S frequently used in CLSM 11,25 and in leakage studies. 26,27 Rhodamine has excellent phototophysic properties such as high levels of absorption coefficient and quantum yield. 28 The specific amount of dye was selected by empirical method 25 and 0.3 mg/mL was chosen as the appropriate concentration for endodontic AH Plus sealer studies, as reported by our pilot study.…”

Effect of Intra-Orifice Depth on Sealing Ability of Four Materials in the Orifices of Root-Filled Teeth: An Ex-Vivo Study

“…Kubo et al [30] reported that dyes or radioisotopes are used in 82% of marginal leakage studies. When they investigated the effect of endodontic materials on the optical density of dyes used in marginal leakage studies, they found no significant statistical difference among methylene blue, indian ink, or rhodamine B dye solutions evaluated.…”