The effect of electrospun scaffolds on the glycosaminoglycan profile of differentiating neural stem cells

Garrudo, Fábio F. F.; Mikael, Paiyz E.; Xia, Ke; Silva, João Carlos; Ouyang, Yilan; Chapman, Caitlyn A.; Hoffman, Pauline R.; Yu, Yanlei; Han, Xiaurui; Rodrigues, Carlos Alberto Chirano; Morgado, Jorge; Ferreira, Frederico Castelo; Linhardt, Robert J.

doi:10.1016/j.biochi.2021.01.001

Cited by 13 publications

(11 citation statements)

References 80 publications

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“…Previous studies focusing on neural progenitor cell supporting scaffolds reported the importance of hydrogel properties such as elastic modulus on their differentiation success and the suitability of topographical cues for the successful alignment of neural cells [ 17 , 38 , 39 ]. For many implemented neuronal models, the used scaffolds either lack topographical cues [ 40 , 41 , 42 ] to replicate the anisotropic nature of spinal cord, or the stiffness for tissue-relevant substrates [ 16 , 43 ]. Our composite fiber–hydrogel scaffolds provide the appropriate stiffness as well as alignment cues to support both the differentiation of the neural progenitor cell line ReN cell VM into neurons and their directional alignment.…”

Section: Discussionmentioning

confidence: 99%

“…To engineer hierarchically structured scaffolds, the controlled deposition of fibers is crucial and several techniques for the alignment of electrospun fibers have been developed, including a rotating drum [ 7 , 8 , 9 ], micro patterned collectors [ 10 ], electrospinning and photolithography [ 11 ] or near-field electrospinning [ 12 ]. It has been shown that axonal alignment of neurons can be triggered and neurite outgrowth can be promoted on submicron electrospun poly(ε-caprolactone) (PCL) fiber scaffolds [ 9 , 13 , 14 , 15 , 16 , 17 ]. PCL has been shown to be biocompatible, suitable for electrospinning, capable to guide neural cells in vitro, and is approved by the FDA [ 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

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Directional Submicrofiber Hydrogel Composite Scaffolds Supporting Neuron Differentiation and Enabling Neurite Alignment

Mungenast¹,

Züger

Selvi

et al. 2022

IJMS

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Cell cultures aiming at tissue regeneration benefit from scaffolds with physiologically relevant elastic moduli to optimally trigger cell attachment, proliferation and promote differentiation, guidance and tissue maturation. Complex scaffolds designed with guiding cues can mimic the anisotropic nature of neural tissues, such as spinal cord or brain, and recall the ability of human neural progenitor cells to differentiate and align. This work introduces a cost-efficient gelatin-based submicron patterned hydrogel–fiber composite with tuned stiffness, able to support cell attachment, differentiation and alignment of neurons derived from human progenitor cells. The enzymatically crosslinked gelatin-based hydrogels were generated with stiffnesses from 8 to 80 kPa, onto which poly(ε-caprolactone) (PCL) alignment cues were electrospun such that the fibers had a preferential alignment. The fiber–hydrogel composites with a modulus of about 20 kPa showed the strongest cell attachment and highest cell proliferation, rendering them an ideal differentiation support. Differentiated neurons aligned and bundled their neurites along the aligned PCL filaments, which is unique to this cell type on a fiber–hydrogel composite. This novel scaffold relies on robust and inexpensive technology and is suitable for neural tissue engineering where directional neuron alignment is required, such as in the spinal cord.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Directional Submicrofiber Hydrogel Composite Scaffolds Supporting Neuron Differentiation and Enabling Neurite Alignment

Mungenast¹,

Züger

Selvi

et al. 2022

IJMS

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“…By manufacturing 3D cylindrical scaffolds from biodegradable and biocompatible polymers that create an environment that mimetic the structure and components of autologous nerve [49]- [53]. Carbon nanotubes (CNT) and conductive polymers such as polyaniline have been employed as a conductor material to improve neural signaling [50], [52].…”

Section: Neural Tissue Engineeringmentioning

confidence: 99%

“…These scaffolds cultured in vitro with neurons, rat Schwann cells and stem cells, showed mimicking the epineurium layer protected nerve, supported the growth and improvement cells differentiation and proliferation into the neuron, scaffolds enhanced the regeneration of nerve (Table 5) [49]- [55]. Garrudo et. al.…”

Section: Neural Tissue Engineeringmentioning

confidence: 99%

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Wet Electrospinning and its Applications: A Review

Suaza

Henao

2022

TecnoL.

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In wet electrospinning, a natural or synthetic polymer solution is deposited on a non-solvent liquid coagulant used as collector. This technique can create 3D nanofiber scaffolds with better properties (e.g., porosity and high surface area) than those of traditional 2D scaffolds produced by standard electrospinning. Thanks to these characteristics, wet electrospinning can be employed in a wide range of tissue engineering and industrial applications. This review aims to broaden the panorama of this technique, its possible fields of action, and its range of common materials. Moreover, we also discuss its future trends. In this study, we review papers on this method published between 2017 and 2021 to establish the state of the art of wet electrospinning and its most important applications in cardiac, cartilage, hepatic, wound dressing, skin, neural, bone, and skeletal muscle tissue engineering. Additionally, we examine its industrial applications in water purification, air filters, energy, biomedical sensors, and textiles. The main results of this review indicate that 3D scaffolds for tissue engineering applications are biocompatible; mimic the extracellular matrix (ECM); allow stem cell viability and differentiation; and have high porosity, which provides greater cell infiltration compared to 2D scaffolds. Finally, we found that, in industrial applications of wet electrospinning: (1) additives improve the performance of pure polymers; (2) the concentration of the solution influences porosity and fiber packing; (3) flow rate, voltage, and distance modify fiber morphology; (4) the surface tension of the non-solvent coagulant on which the fibers are deposited has an effect on their porosity, compaction, and mechanical properties; and (5) deposition time defines scaffold thickness.

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Electrospun Nanofibrous Scaffolds for Neural Tissue Engineering

Pramanik

Muthuvijayan

2022

Electrospun Polymeric Nanofibers

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The effect of electrospun scaffolds on the glycosaminoglycan profile of differentiating neural stem cells

Cited by 13 publications

References 80 publications

Directional Submicrofiber Hydrogel Composite Scaffolds Supporting Neuron Differentiation and Enabling Neurite Alignment

Directional Submicrofiber Hydrogel Composite Scaffolds Supporting Neuron Differentiation and Enabling Neurite Alignment

Wet Electrospinning and its Applications: A Review

Electrospun Nanofibrous Scaffolds for Neural Tissue Engineering

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