The effect of electromagnetic fields on survival and proliferation rate of dental pulp stem cells

Samiei, Mohammad; Aghazadeh, Zahra; Abdolahinia, Elaheh Dalir; Vahdati, Amin; Daneshvar, Sabalan; Noghani, Atefe

doi:10.1080/00016357.2020.1734655

Cited by 15 publications

(18 citation statements)

References 37 publications

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“…A similar kind of observation was reported by Samiei et al, where they have demonstrated the higher proliferation of hDPSCs in the presence of sinusoidal EMFs. 1 Furthermore, we have also evaluated the cell morphology in 5 V EMF-treated groups aer 3 days of culture. The cell morphology of the SCAPs was examined by uorescence microscopy, and the results are given in Fig.…”

Section: Emf Stimulation Improves the Proliferation And Migration Of Scapsmentioning

confidence: 99%

“…The effects of electromagnetic elds (EMFs) have been shown to ameliorate bone healing for a long time. [1][2][3] The exogenous EMFs can interfere with various biological processes, including proliferation, 4 morphology, 5 stemness, 1,3 gene expression, 6 protein expression, 7 apoptosis, 8 and cell differentiation. 7 Chronic wound response usually generates naturally occurring endogenous EFs that play a crucial role in active cell migration from bone marrow to the wounded site by a process known as electrotaxis (ET).…”

Section: Introductionmentioning

confidence: 99%

“…13,14 Mesenchymal stem cells (MSCs) are a promising treatment option compared to the conventional treatment methods in dentistry. 1,15 Adult stem cells are multipotent cells capable of differentiation in multiple lineages, such as osteocytes, chondrocytes, adipocytes, and neuron-like cells, respectively. 15,16 In addition, stem cell-based tissue regeneration offers several advantages, including the avoidance of complex surgeries, low risk of contamination, high quality of tissue regeneration, and low rate of autoimmune rejection.…”

Section: Introductionmentioning

confidence: 99%

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Section: Emf Stimulation Improves the Proliferation And Migration Of Scapsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Electromagnetic field-assisted cell-laden 3D printed poloxamer-407 hydrogel for enhanced osteogenesis

et al. 2021

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“…Similarly, PEMFs promote neurite outgrowth and differentiation in embryonic neural stem cells by recruiting the activity of TRPC1 channels (Ma et al, 2016). Quite recently, moreover, PEMF treatment also promotes the survival and proliferation of hDPSCs (Samiei et al, 2020). Nonetheless, PEMF-induced proliferation of hDPSCs required hours of treatment delivered over a week (Samiei et al, 2020), severely undermining their practical implementation in clinical environments.…”

mentioning

confidence: 99%

“…Quite recently, moreover, PEMF treatment also promotes the survival and proliferation of hDPSCs (Samiei et al, 2020). Nonetheless, PEMF-induced proliferation of hDPSCs required hours of treatment delivered over a week (Samiei et al, 2020), severely undermining their practical implementation in clinical environments. The present study showed that by combining PEMF intervention with 2DG, targeted to a time when TRPC1 channels are most developmentally expressed in vitro, it was possible to reduce the requisite PEMF exposure time to only 10 min applied once, while simultaneously accelerating and enhancing hDPSCderived neurogenesis.…”

mentioning

confidence: 99%

Pulsed electromagnetic fields synergize with graphene to enhance dental pulp stem cell-derived neurogenesis by selectively targeting TRPC1 channels

Madanagopal¹,

Tai²,

Lim³

et al. 2021

eCM

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Conventional root canal treatment replaces the infected pulp with defined materials. Alternative cell-based tissue engineering strategies aim to regenerate a fully functional pulp within the root canal. Despite recent advances in this area, however, the regeneration of an innervated pulp remains a major challenge in the field. Both graphene (2DG) and pulsed electromagnetic fields (PEMFs) independently have been shown to promote diverse cellular developmental programs. The present study showed that 2DG promoted the neurogenic induction of human dental pulp stem cells (hDPSCs) by upregulating and accelerating the expression of mature neuronal markers. Notably, 2DG induced the highest expression of transient receptor potential canonical cation channel type 1 (TRPC1) during early neurogenesis. As brief PEMF exposure promotes in vitro differentiation by activating a TRPC1-mitochondrial axis, an opportunity to combine 2DG with developmentally targeted PEMF exposure for synergistic effects was realizable. Neurogenic gene expression, neurotransmitter release, and reactive oxygen species (ROS) production were greatly enhanced by a brief (10 min) and low amplitude (2 mT) PEMF exposure timed to coincide with the highest TRPC1 expression from hDPSCs on 2DG. In contrast, hDPSCs on glass were less responsive to PEMF exposure. The capacity of PEMFs to promote neurogenesis was precluded by the administration of penicillin/streptomycin, mirroring previous studies demonstrating that aminoglycoside antibiotics block TRPC1-mediated calcium entry and verifying the contribution of TRPC1 in this form of magnetoreception. Hence, graphene created a more conducive environment for subsequent PEMF-stimulated neurogenic induction of hDPSCs through their mutual capacity to activate TRPC1with subsequent ROS production.

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Biotechnological Effects of Advanced Smart‐Bile Acid Cyclodextrin‐Based Nanogels for Ear Delivery and Treatment of Hearing Loss

Kovacevic,

Wagle,

Ionescu

et al. 2024

Adv Healthcare Materials

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Inner ear delivery requires safe and effective drug delivery vehicles incorporating high‐viscosity formulations with permeation enhancers. This study designs novel thermoresponsive‐smart polymer‐bile acid and cyclodextrin‐based nanogels for inner ear delivery. Nanogels are examined for their rheological and physical properties. The biocompatibility studies will be assessed on auditory and macrophage cell lines by investigating the impact of nanogels on cellular viability, mitochondrial respiration, glycolysis, intracellular oxidative stress, inflammatory profile, and macrophage polarization. Novel ther nanogels based on bile acid and beta‐cyclodextrin show preserved porous nanogels' inner structure, exhibit non‐Newtonian, shear‐thinning fluid behavior, have fast gelation at 37 °C and minimal albumin adsorption on the surface. The nanogels have minimal impact on cellular viability, mitochondrial respiration, glycolysis, intracellular oxidative stress, and inflammatory profile of the auditory cell line House Ear Institute‐Organ of Corti 1 after 24 h incubation. Nanogel exposure of 24 h to macrophage cell line RAW264.7 leads to decreased viability, mitochondrial dysfunction, and increased intracellular ROS and inflammatory cytokines. However, polarization changes from M2 anti‐inflammatory to M1 pro‐inflammatory macrophages are minimal, and inflammatory products of RAW264.7 macrophages do not overly disrupt the survivability of HEI‐OC1 cells. Based on these results, thermoresponsive bile acid and cyclodextrin nanogels can be potential drug delivery vehicles for inner ear drug delivery.

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The effect of electromagnetic fields on survival and proliferation rate of dental pulp stem cells

Cited by 15 publications

References 37 publications

Electromagnetic field-assisted cell-laden 3D printed poloxamer-407 hydrogel for enhanced osteogenesis

Electromagnetic field-assisted cell-laden 3D printed poloxamer-407 hydrogel for enhanced osteogenesis

Pulsed electromagnetic fields synergize with graphene to enhance dental pulp stem cell-derived neurogenesis by selectively targeting TRPC1 channels

Biotechnological Effects of Advanced Smart‐Bile Acid Cyclodextrin‐Based Nanogels for Ear Delivery and Treatment of Hearing Loss

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