The Effect of Early Rounds of ex vivo Expansion and Cryopreservation on the Adipogenic Differentiation Capacity of Adipose-Derived Stromal/Stem Cells

Durandt, Chrisna; Dessels, Carla; Silva, C. Da; Murdoch, Candice; Pepper, Michael Sean

doi:10.1038/s41598-019-52086-9

Cited by 6 publications

(7 citation statements)

References 69 publications

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“…Interestingly, many of these proteins were associated with cartilage development and the ECM of elastic cartilage, including aggrecan (ACAN), 29 elastin microfibril interfacer 1 (EMILIN1), 30 chondroadherin (CHAD), 30 matrilin‐3 (MATN3), 31 collagen type 8 alpha chain 1 (COL8A1), 32 prolargin (PRELP), tenascin (TNC), and asporin (ASPN) 33 . One of the few proteins enhanced on the DAT microcarriers was CD36, which is a marker of adipose progenitor cells 34 . Volcano plots highlighting the proteins significantly upregulated in the DAT or DCT groups in comparison to the TCP group can be found in Supplementary Figure S4 and Supplementary Figure S5.…”

Section: Resultsmentioning

confidence: 99%

“…It is well recognized that the differentiation potential of MSCs decreases through passaging and expansion on 2D TCP 34,67 . Based on previous studies, it is possible that both compliant substrates 68 and dynamic culture can modulate the capacity of MSCs to differentiate 69 .…”

Section: Discussionmentioning

confidence: 99%

“…33 One of the few proteins enhanced on the DAT microcarriers was CD36, which is a marker of adipose progenitor cells. 34 Volcano plots highlighting the proteins significantly upregulated in the DAT or DCT groups in comparison to the TCP group can be found in Supplementary Figure S4 and Supplementary Figure S5. Confirming that the FACS isolation method yielded highly purified cell populations and that the presence of residual scaffold debris was not contributing to the enhanced presence of cartilagespecific proteins in the DCT group, similar protein intensities were measured for a range of ECM and cellular proteins in both ECMderived microcarrier groups relative to the baseline TCP samples (Supplementary Figure S6).…”

Section: Proteomic Analysis Of Hascs Cultured Dynamically On the Dat ...mentioning

confidence: 99%

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Probing the effects of matrix‐derived microcarrier composition on human adipose‐derived stromal cells cultured dynamically within spinner flask bioreactors

Kornmuller

Cooper

Jani

et al. 2022

J Biomedical Materials Res

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Recognizing the cell‐instructive capacity of the extracellular matrix (ECM), this study investigated the effects of expanding human adipose‐derived stromal cells (hASCs) on ECM‐derived microcarriers fabricated from decellularized adipose tissue (DAT) or decellularized cartilage tissue (DCT) within spinner flask bioreactors. Protocols were established for decellularizing porcine auricular cartilage and electrospraying methods were used to generate microcarriers comprised exclusively of DAT or DCT, which were compositionally distinct, but had matching Young's moduli. Both microcarrier types supported hASC attachment and growth over 14 days within a low‐shear spinner culture system, with a significantly higher cell density observed on the DCT microcarriers at 7 and 14 days. Irrespective of the ECM source, dynamic culture on the microcarriers altered the expression of genes and proteins associated with cell adhesion and ECM remodeling. Label‐free mass spectrometry analysis showed upregulation of proteins associated with cartilage development and ECM in the hASCs expanded on the DCT microcarriers. Based on Luminex analysis, the hASCs expanded on the DCT microcarriers secreted significantly higher levels of IL‐8 and PDGFAA, supporting that the ECM source can modulate hASC paracrine factor secretion. Finally, the hASCs expanded on the microcarriers were extracted for analysis of adipogenic and chondrogenic differentiation relative to baseline controls. The microcarrier‐cultured hASCs showed enhanced intracellular lipid accumulation at 7 days post‐induction of adipogenic differentiation. In the chondrogenic studies, a low level of differentiation was observed in all groups. Future studies are warranted using alternative cell sources with greater chondrogenic potential to further assess the chondro‐inductive properties of the DCT microcarriers.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Proteomic Analysis Of Hascs Cultured Dynamically On the Dat ...mentioning

confidence: 99%

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Probing the effects of matrix‐derived microcarrier composition on human adipose‐derived stromal cells cultured dynamically within spinner flask bioreactors

Kornmuller

Cooper

Jani

et al. 2022

J Biomedical Materials Res

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“…ADSCs from old donors exhibited some changes, such as natural aging. Moreover, ADSCs can stand long-term cryopreservation with a high survival rate after resuscitation, without a significant impact on their proliferative and differentiation abilities [ 56 , 57 ]. Therefore, we suggest that ADSCs should be cryopreserved in youth with a minimum number of passages to make autologous transplantation work better for senile diseases.…”

Section: Discussionmentioning

confidence: 99%

Age-related alteration in characteristics, function, and transcription features of ADSCs

Shi

Lei

et al. 2021

Stem Cell Res Ther

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Background and objectives Adipose tissue-derived stem cells (ADSCs) autologous transplantation has been a promising strategy for aging-related disorders. However, the relationship between ADSCs senescence and organismal aging has not been clearly established. Therefore, we aimed at evaluating senescence properties of ADSCs from different age donors and to verify the influence of organismal aging on the proliferation and function of ADSCs in vitro, providing the theoretical basis for the clinical application of autologous ADSCs transplantation. Methods and results The ADSCs were obtained from 1-month-old and 20-month-old mice. The cells characteristics, functions, gene expression levels, apoptosis proportion, cell cycle, SA-β-gal staining, and transcription features were evaluated. Compared to ADSCs from 1-month-old mice, ADSCs from 20-month-old mice exhibited some senescence-associated changes, including inhibited abilities to proliferate. Moreover, differentiation abilities, cell surface markers, and cytokines secreting differed between 1M and 20M ADSCs. SA-β-Gal staining did not reveal differences between the two donor groups, while cells exhibited more remarkable age-related changes through continuous passages. Based on transcriptome analysis and further detection, the CCL7-CCL2-CCR2 axis is the most probable mechanism for the differences. Conclusions ADSCs from old donors have some age-related alterations. The CCL7-CCL2-CCR2 axis is a potential target for gene therapy to reduce the harmful effects of ADSCs from old donors. To improve on autologous transplantation, we would recommend that ADSCs should be cryopreserved in youth with a minimum number of passages or block CCL7-CCL2-CCR2 to abolish the effects of age-related alterations in ADSCs through the Chemokine signaling pathway.

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“…Moreover, ASCs isolated from young or old donors do not significantly differ in the cell surface antigen profile. However, obesity results in decreased expression of stemness markers compared to those isolated from lean donors [ 13 ].…”

Section: Biology Of Adipose Tissuementioning

confidence: 99%

Adipose Tissue-Derived Mesenchymal Stem Cells

Bunnell

2021

Cells

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The long-held belief about adipose tissue was that it was relatively inert in terms of biological activity. It was believed that its primary role was energy storage; however, that was shattered with the discovery of adipokines. Scientists interested in regenerative medicine then reported that adipose tissue is rich in adult stromal/stem cells. Following these initial reports, adipose stem cells (ASCs) rapidly garnered interest for use as potential cellular therapies. The primary advantages of ASCs compared to other mesenchymal stem cells (MSCs) include the abundance of the tissue source for isolation, the ease of methodologies for tissue collection and cell isolation, and their therapeutic potential. Studies conducted both in vitro and in vivo have demonstrated that ASCs are multipotent, possessing the ability to differentiate into cells of mesodermal origins, including adipocytes, chondrocytes, osteoblast and others. Moreover, ASCs produce a broad array of cytokines, growth factors, nucleic acids (miRNAs), and other macromolecules into the surrounding milieu by secretion or in the context of microvesicles. The secretome of ASCs has been shown to alter tissue biology, stimulate tissue-resident stem cells, change immune cell activity, and mediate therapeutic outcomes. The quality of ASCs is subject to donor-to-donor variation driven by age, body mass index, disease status and possibly gender and ethnicity. This review discusses adipose stromal/stem cell action mechanisms and their potential utility as cellular therapeutics.

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The Effect of Early Rounds of ex vivo Expansion and Cryopreservation on the Adipogenic Differentiation Capacity of Adipose-Derived Stromal/Stem Cells

Cited by 6 publications

References 69 publications

Probing the effects of matrix‐derived microcarrier composition on human adipose‐derived stromal cells cultured dynamically within spinner flask bioreactors

Probing the effects of matrix‐derived microcarrier composition on human adipose‐derived stromal cells cultured dynamically within spinner flask bioreactors

Age-related alteration in characteristics, function, and transcription features of ADSCs

Adipose Tissue-Derived Mesenchymal Stem Cells

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