The effect of duration of compression on lipid peroxidation after experimental spinal cord injury

Kaynar, Mehmet Yaşar; Hancı, Murat; Kafadar, Ali; Gümüştaş, Koray; Belce, Ahmet; Çiplak, Nejat

doi:10.1007/bf02389316

Cited by 16 publications

(19 citation statements)

References 30 publications

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“…36 In this study, thiobarbituric acid test to measure MDA in homogenates was used because it was the test that we have been familiar with. 37,38 Although the reaction of TBA with a complex variety of other molecules also provides a positive reaction, this can be acceptable if the results are compared with baseline lipid peroxidation levels obtained from the sham-operated group. The accuracy and appropriateness of the MDA test to measure the extent of lipid peroxidation in experimental conditions has been accepted by many investigators.…”

Section: Discussionmentioning

confidence: 99%

The effect of epidural cooling on lipid peroxidation after experimental spinal cord injury

et al. 1998

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Study Design: The e ect of epidural space perfusion with chilled saline solution (% 0.9 NaCl) on lipid peroxidation after experimental spinal cord injury in rats was evaluated. Objectives: The extent of lipid peroxidation is a useful parameter for evaluating the cellular disturbance caused by spinal cord trauma in experimental conditions. The protective e ects of hypothermia against neurological injury resulting from trauma or ischemia both in experimental and clinical situations have been demonstrated. Setting: Departments of Neurosurgery and Biochemistry, Cerrahpasa Medical School, Istanbul, Turkey. Methods: Twenty-®ve female Wistar Albino rats were used. There were ®ve rats in group I (sham-operated), seven rats in group II (trauma), and eight rats in group III (epidural cooling). The remaining ®ve rats were used for the pilot study to determine the spinal cord and body temperature. A clip compression method was used to produce acute spinal cord injury. In group III, 30 min after the trauma the injured spinal cord was cooled by perfusion of the epidural space with chilled saline solution (% 0.9 NaCl) with a¯ow rate of 5 ml/min for 30 min. At 2 h after trauma, all rats other than the ones used in the pilot study, were sacri®ced and the spinal cords were excised. The extent of lipid peroxidation in the spinal cord was assessed by measuring the tissue content of malonil dialdehyde (MDA). Results: The tissue MDA contents were 1.58 micromol MDA/gram wet weight (gww) in group 1 (sham-operated), 2.58 micromol MDA/gww in group 2 (trauma), and 1.77 micromol/ gww in group 3 (epidural cooling), the di erences being statistically signi®cant. Conclusion:The results indicated that epidural cooling of traumatized spinal cord is e ective in preventing secondary damage due to the peroxidation of lipid membranes.

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Section: Discussionmentioning

confidence: 99%

The effect of epidural cooling on lipid peroxidation after experimental spinal cord injury

et al. 1998

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“…The factors that play a role in this progressive process have not been fully elucidated. In addition to excessive release of glutamate and aspartate, intracellular calcium accumulation, activation of the arachidonic acid cascade, activation of various proteases such as caspase phospholipase-endonuclease, and induction of the peroxidation of lipids of free radical origin (30, 41,78), sympathetic stimulation also contributes to secondary injury mechanisms through altered blood flow and changes in microvascular permeability although the mechanism is not fully understood (4,14). Rapid neutrophil migration occurs to the injured region (9).…”

Section: Evaluation Of Motor Strengthmentioning

confidence: 99%

The biochemical, histopathological and clinical comparison of the neuroprotective effects of subcutaneous adalimumab and intravenous methylprednisolone in an experimental compressive spinal cord trauma model

Çelik

Karatay²,

Yavuz³

et al. 2015

Turkish Neurosurgery

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AIM:To evaluate the neuroprotective effects of adalimumab in an experimental spinal cord injury model and compare them with those of the widely-used methylprednisolone. MATERIAL and METHODS:Forty male Wistar rats were divided into 5 as the sham, trauma, adalimumab, methylprednisolone, and adalimumab+methylprednisolone groups. Only laminectomy was performed in the sham group. Laminectomy and trauma was performed to the trauma group but no treatment was given. A single dose of 40 mg/kg subcutaneous adalimumab was administered after the laminectomy and trauma to group 3. A single dose of intravenous 30 mg/kg methylprednisolone was administered right after laminectomy and trauma to group 4. Single doses of 40 mg/kg adalimumab and 30 mg/kg methylprednisolone were administered together after laminectomy and trauma to group 5. Serum malondialdehyde (MDA), TNF-α, IL-1β and IL-6 levels were measured and sections were obtained for histopathological study at the end of the 7 th day. RESULTS:MDA, TNF-α, IL-1β and IL-6 levels in serum were significantly decreased in the adalimumab group with clinical and histopathological improvement not less than the methylprednisolone group. The serum MDA levels were similar when the two drugs were given together or separately but there was a statistically quite significant decrease in TNF-α, IL-1β and IL-6 levels with concurrent use. Statistically significantly better results were obtained on histopathological evaluation with the use of both drugs together. CONCLUSION:This study revealed that adalimumab is as effective as methylprednisolone in compressive spinal cord injury in rats.

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“…The former results in immediate injuries of the cervical spinal cord, while the latter results in delayed but prolonged injuries. Persistent compression is one ofthe main causes of secondary SCI mechanisms; the severity of SCI depends on the dtiration of compression to some extent [21]. The extent of compression to spinal cord can be showed in MRI generally, although, there are some situations when neurological functions of spinal cord may be abnormal without any abnormalities showed in MRI [22].…”

Section: Discussionmentioning

confidence: 99%

Risk Factors of Iatrogenic Spinal Cord Injury in Spinal Surgery: A Multicenter Retrospective Study

Chen

2012

International Journal of Neuroscience

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The effect of duration of compression on lipid peroxidation after experimental spinal cord injury

Cited by 16 publications

References 30 publications

The effect of epidural cooling on lipid peroxidation after experimental spinal cord injury

The effect of epidural cooling on lipid peroxidation after experimental spinal cord injury

The biochemical, histopathological and clinical comparison of the neuroprotective effects of subcutaneous adalimumab and intravenous methylprednisolone in an experimental compressive spinal cord trauma model

Risk Factors of Iatrogenic Spinal Cord Injury in Spinal Surgery: A Multicenter Retrospective Study

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