The Effect of Duration of Use of Depomedroxyprogesterone Acetate on the Thickness of the Vaginal Epithelium of Mice

Abstract

BACKGROUND: Depomedroxyprogesterone acetate (DMPA) is one of the most effective contraceptive methods currently widely used. Injectable contraceptives, including DMPA, are growing in popularity because of their ease of use, effectiveness and affordability. The use of DMPA also has advantages such as not interfering with sexual relations, does not need male participation, does not require storage space, and is easy to obtain and helps increase body weight.
 AIM: The aim of the study was to analyze the effect of the duration of use of DMPA on the thickness of the vaginal epithelium of mice.
 METHODS: The research design used in this study was true experimental with a post-test only control group design approach. This research was carried out treatment at a dose of 0.39 mg/mouse/day DMPA exposure in mice. The phenomenon observed in this study was the thickness of the vaginal epithelium of mice. This study used mice with the completely randomized design method and was carried out at the Pharmacology Laboratory and the Anatomical Pathology Laboratory, FKH Unsyiah. The collection of data technique was conducted by observation with a light microscope to calculate the average thickness of the vaginal epithelium of mice injected with DMPA in the four groups. The data analysis technique was carried out in five stages, namely, the normality test of the sample data with the Shapiro–Wilk test, the homogeneity test of variance with the Levene test, and comparing the thickness of the vaginal epithelium in each of the two treatment groups using the independent t-test.
 RESULTS: The finding revised that there was a significant difference in the thickness of the vaginal epithelium in the two treatment groups 10 days and 15 days of DMPA exposure which was indicated by a p = 0.003 (p < 0.05). DMPA exposure levels cause thinning of the vaginal epithelial thickness. The average thickness of vaginal epithelium exposure to DMPA for 10 days in the control group was 182536.33 ± 5773.465. In the treatment group, exposure to DMPA for 10 days, the thickness of the vaginal epithelium was 168322.33 ± 7611.300. The mean vaginal epithelial thickness in the 15-day DMPA exposure group in the control group was 177242.83 ± 9689.558. In the 15-day DMPA exposure treatment group, the thickness of the vaginal epithelium was 138674.83 ± 17176.124.
 CONCLUSION: The application of social marketing has not been effective in changing the smoking behavior of students in Aceh. The application of social marketing needs to be supported by internal and external Islamic Boarding Schools.

Peningkatan Pengetahuan Wanita Usia Subur Tentang Efek Penggunaan Kontrasepsi Progestin Terhadap Kesehatan Organ Reproduksi

“…Depo Medroxyprogesterone Acetate (DMPA) adalah salah satu metode kontrasepsi suntik yang mengandung hormone progesteron dengan interval suntik ulang selama 3 bulan (FSRH, 2020). Efek samping utama dari kontrasepsi DMPA pada pemakaian lebih dari 2 tahun adalah gangguan menstruasi berupa amenorea 52,7% dan mengalami peningkatan berat badan 58,1%, sakit kepala 13,6% (Rahayu & Wijanarko, 2017;Harahap & Amelia, 2020), hiperkolesterolmia (Fazdria, 2020Maydawati & Veri, 2020) dan penipisan epitel vagina (Veri et al, 2021).…”

“…Infeksi HIV pada perempuan paling banyak terdapat pada kelompok perempuan berusia produktif (Kharin Herbawani et al, 2019). Hasil penelitian menyatakan semakin lama paparan DMPA akan menyebabkan lapisan epitel vagina menjadi tipis (Veri et al, 2021). Berdasarkan latar belakang tersebut, penulis tertarik melakukan kegiatan pengabdian kepada masyarakat tentang edukasi pemakaian DMPA Terhadap Ketebalan Epitel Vagina pada WUS akseptor KB DMPA di desa Paya Bujok Teungoh Langsa Barat Kota Langsa.…”

“…Informasi tentang KB DMPA sangat diperlukan karena hasil penelitian yang telah dilakukan menunjukkan pemaparan DMPA akan menipiskan ketebalan epitel vagina akibat pemaparan DMPA selama 10 hari dan 15 hari (Veri et al, 2021). Penggunaan DMPA meningkatkan terjadinya disfungsi endotel (Veri et al, 2019) dan menurunkan reseptor estrogen (Fitrianingtyas & Anggreni, 2019).…”

“…Paparan DMPA mempengaruhi dilatasi dan distribusi pembuluh darah pada permukaan. Dilatasi pembuluh darah akan mengakibatkan vaskular kekurangan komponen pada membran dan hal ini diduga menjadi penyebab meningkatnya kerapuhan pada vaskular yang pada akhirnya memicu penipisan epitel vagina pada pengguna DMPA (Emilda et al, 2017;Veri et al, 2021).…”